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MshOt MF52-N, User Manual

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MF52-N

Inverted Fluorescence Microscope

User Manual

Guangzhou Micro-shot Technology Co., Ltd

Website: www.m-shot.com

Email: [email protected]

Summary of Contents for MF52-N

Page 1: ...MF52 N Inverted Fluorescence Microscope User Manual Guangzhou Micro shot Technology Co Ltd Website www m shot com Email sales m shot com ...

Page 2: ...Inverted Fluorescence Microscope MF52 N ...

Page 3: ...n improper bulb fuse or power cord may cause damage or fire to this unit Any extended power cord used must be grounded PE 5 Protect this unit from high temperatures dampness and foreign objects To prevent short circuit or any other fault do not expose this unit to any high temperatures or dampness environment for a prolonged period of time A suitable operating environment is designated at a temper...

Page 4: ...ulb Do not touch the glass surface of the bulb directly with bare hands When mounting the bulb wear gloves or wrap it with cotton material Wipe off any dirt on the surface of the bulb with a clean cotton fabric dipped in alcohol If the dirt is not thoroughly removed it would etch the surface of the bulb weakening its brightness and shortening its life Mount the bulb with care to avoid slipping off...

Page 5: ...stallation 1 IV Technical specification 错误 未定义书签 V Operation 错误 未定义书签 Bright field observation 4 Phase contrast observation 8 Fluorescence observation 9 Operation of the photographic device 9 VI Replace Bulb and Fuse 错 误 未定义书签 VII Maintenance 错 误 未 定 义 书 签 VIII Troubleshooting 11 ...

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Page 7: ...do non infected culture cell observation in high culture dish and cylindrical flask The epi fluorescence system adopts a module design philosophy and can adjust the illumination system as well as switching fluorescence filter The machine can be applied in cell tissue and transparent liquid tissue observation as well as bio medical medical detection and disease prevention II Structure and features ...

Page 8: ...ff dust cover cap from eyepiece holder put two eyepieces 4 into the holder and rotate to make them matched well 4 Unload the moving board and put the round glass stage board 2 in the middle of the stage if not needed 5 If the moving board is needed default with installed from factory select suitable culture dish board and put it in the moving board frame 6 Connect power line to microscope body and...

Page 9: ...tion wavelength 510nm 550nm Phase contrast ring plate 10X 20X and 40X in one unit Focus system Coaxial coarse fine focus with tension adjustable and up stop minimum division of fine focusing is 2μm Nosepiece Quintuple nosepiece ball bearing with anti fungus device Stage Fixed stage overall size is 227mmX208mm Glass rotundity stage overall size is Ф118mm inner size is Ф68mm Mechanical moving device...

Page 10: ...t path as figure 04 shows 3 Reset diopter ring Rotate the diopter ring 1 leave the FOV 0 align at the side mark line Figure05 Check if the input voltage is consistent with your local voltage supply If not do not operate this unit and contact the supplier Improper input voltage may cause a short circuit or fire thereby causes damage to this unit Figure 03 1 Figure 04 2 1 Long term high brightness w...

Page 11: ...ze of the dish Refer to the specification figure See Figure 06 Figure 06 1 2 Adjust the hand wheel 1 and 2 The observation area should be right above the objective for convenient observation See figure 07 6 coarse fine focusing 1 Use the 10X objective to focus Rotate the nosepiece 1 put the 10 x objective in the light path See figure 08 2 Rotate the coarse hand wheel 3 or 6 raise the objective to ...

Page 12: ...s figure 09 It will hard to work if adjust coarse focusing too much Notice It will caused self protected status when moving stage ruler moved to its limitation components will rub against each other and lead the stage stop working Notice Figure 09 1 2 3 Figure 08 1 2 3 4 5 6 Use the 10X objective to focus and set location When change to higher magnification use the coarse hand wheel to raise the o...

Page 13: ...verse it and do the steps below a Y direction Unscrew screw 4 unload the supporting board 3 unscrew the two screws 1 a little bit and then adjust screw 2 with Allen wrench to adjust the tightness of Y transmission steel rope See figure 11 a b X direction Unscrew the two screws 5 and adjust screw 6 to adjust the tightness of X transmission steel rope See figure 11 b Moving stage ruler is the key pa...

Page 14: ...e 2 into the lens as figure 13 a 2 A dark ring 5 and a bright ring 6 would appear in the field as figure 13 c 3 Adjust ring 1 if the edge of the dark or bright ring is not clear 4 Adjust the plate as figure 13 c shows if the two rings not coincide with Allen wrench 3 Adjust screw 4 if it is a pull plate one See figure 13 d 5 After centering take out centering eyepiece And insert observation eyepie...

Page 15: ...ness controller knob 2 and hear sound Ding the fluorescence light is open 2 Default brightness is the most weak knob 2 can control brightness According to sample observation needs slider 1 is used to transfer between different filter groups LED lamp changed automatically Operation of the photographic device The push pull rod is used to switch observation camera The camera output interface is on th...

Page 16: ... and Fuse This instrument fuse is used to light for transmitted lighting system fuse 3 is integrated at the input power adapter of host as figure 17 1 Replace transmission illumination halogen bulb 1 Turn off the power switch 1 and unplug the power cord 2 as figure 17 2 Take out fuse basement 2 by flat screwdriver 1 as image shows then take off broken fuse and insert new fuse out the fuse basement...

Page 17: ... bulb to cool down sufficiently put on the dust shield store this unit at a dry ventilated and clean place free from any acid alkali or steam otherwise mold may develop on the lens 6 Periodic inspection This unit should be inspected and maintained periodically to maintain its performance VIII Troubleshooting Fault Cause Disposition Electronic system No light shown in the field of view The power sw...

Page 18: ...hick or thickness Prepare sample slider according to objective parameters The focal plane of the image is inclined brighter on one side and darker on the other The lighting bulb is seriously inclined Reposition the lighting bulb The specimen is not laid flatly Lay the specimen flatly on the object stage and hold it stably Surrounding is too bright Create darker surrounding when observing fluoresce...

Page 19: ...o old Check and have professional repair it Mechanical stage Y direction moving is too slightly and leads X direction moving at the same time Adjust Y direction driver wire tightness X direction moving is too slightly and leads Y direction moving at the same time Adjust X direction driver wire tightness Driver wire break Change new driver wire requires professional workshop to repair it ...

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