SpectraMax L Luminescence Microplate Reader User Guide
39
0112-0174 C
Injector Accuracy Test
This gravimetric method tests the accuracy of the injection volume. The method is based
on the weight of water dispensed from the injector.
Weigh the plate using an electronic balance with an accuracy of 0.01 grams. Because
water injected into the plate evaporates, the plate should be sealed following the
injection process. Seal the plate with a commercially available plate sealing tape. Label
tape can be used if plate sealing tape is not available.
To test injector accuracy:
1. Prime the injectors. See
2. Prime the injectors two more times.
3. Measure and record the weight of an empty 96-well plate. Include the plate sealing
material in the weight.
4. Put the weighed plate into the instrument.
5. In the SoftMax Pro Software, select a Plate section in the Navigation Tree and click
to display the Settings dialog.
6. Select the
Endpoint
read type.
7. Select the
Plate Type
category and select the
96-well Standard
plate.
8. Select the
Read Area
category and select the
Select All
check box.
9. Select the
Integration Time
category and enter an Integration Time of 1 second.
10. Select the
Sensitivity
category.
11. Click the
PMT Setting
drop-down and select
AutoRange
.
12. For the
Target Calibration Wavelength
, select the
395 nm
option.
13. Select the
Injection & Delay
category.
14. Select the
M–Injection
check box and enter the following injection parameters.
M-Injector Volume (µL)
- 50
Injection Speed (µL/s)
- 230
15. Click
OK
to close the Settings dialog.
16. Select the Home tab and click
to start the M-injections.
17. When the injections finish, remove, seal, and weigh the plate. Record the weight and
then determine the weight difference between the empty plate and the plate after
the injections.
The weight difference must be 4.80 grams +/- 0.05 grams. If the measured weights
recorded from the above steps are not within 4.80 grams +/- 0.05 grams, you should
calibrate the injectors.
18. Repeat the above steps for P-injection.
Again, the weight difference must be 4.80 grams +/- 0.05 grams. If the measured
weights recorded from the above steps are not within 4.80 grams +/- 0.05 grams, you
should calibrate the injectors.
