Chapter 4: Protocols
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4. Click
Reverse
to reverse the injection cycle pumps and return the reagent from the
tubing to the reservoir.
Protocol Settings
The Settings dialog in the SoftMax Pro Software enables you to define the instrument
settings to read the plate. See the SoftMax Pro Software application help for details.
To define protocol settings:
1. Start the SoftMax Pro Software.
2. Select the SpectraMax L instrument.
Note:
When the computer is not physically connected to an instrument, you
can select Offline or Simulator On to create documents for later use.
Document settings are specific to the instrument that you select. If you change
instruments, some settings may be reset to the default protocol settings.
3. In the Navigation Tree, select a Plate section.
4. Click
in the Settings Information area of the Workspace or select the Home tab
and click
Acquisition Settings
to display the Settings dialog.
5. Select the
Lumi
or
Lumi2W
read mode.
6. Select the
Endpoint
,
Dual Read
,
Kinetic
, or
Fast Kinetic
read type.
7. Select the
Plate Type
category.
a. Click the
Plate Format
drop-down and select
96-Wells
or
384-Wells
.
b. In the
Select Specific
list, select the plate.
8. Select the
Read Area
category.
a. Select the wells to read. Wells that will be read appear shaded dark.
To read all the wells in the plate, select the
Select All
check box.
To select a contiguous region, click and drag the cursor over the wells to read.
To select non-contiguous wells, press
Ctrl
and click the wells to read.
b. Click a well a second time to exclude the well from the read.
