Molecular Devices SpectraMax L User Manual Download Page 1

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0112-0174 C
October 2018

SpectraMax® L

Luminescence Microplate Reader

User Guide

Summary of Contents for SpectraMax L

Page 1: ...0112 0174 C October 2018 SpectraMax L Luminescence Microplate Reader User Guide...

Page 2: ...ht and or license to use or permit others to use such manufacturers and or their product names as trademarks Each product is shipped with documentation stating specifications and other technical infor...

Page 3: ...anging the Plate Aperture 21 Chapter 3 Getting Started 23 Software Ribbon 23 Instrument Calibration 24 Chapter 4 Protocols 26 Temperature Regulation 26 Injector Protocols 26 Protocol Settings 28 Power...

Page 4: ...cular Devices the protection provided by the equipment might be impaired Warnings Cautions Notes and Tips All warning symbols are framed within a yellow triangle An exclamation mark is used for most w...

Page 5: ...ion of the instrument can involve the use of materials that are toxic flammable or otherwise biologically harmful When you use such materials observe the following precautions Handle infectious sample...

Page 6: ...nge labware reagents or tools while the instrument is operating Never try to physically restrict the moving components of the instrument WARNING Do not attempt to access the interior of the instrument...

Page 7: ...e When the instrument has two PMTs and no filters the instrument runs Lumi protocols that use both PMTs to read 2 wells simultaneously and it assumes the readings of the PMTs are comparable Lumi2W Lum...

Page 8: ...itoring between injection and measurement provides for extremely fast reactions and reduced delay time 96 well Standard plates 10 L to 200 L 384 well and 96 well half area plates 10 L to 80 L Optional...

Page 9: ...llation Guide To prevent data loss turn off all sleep and hibernation settings for the hard disk the CPU and the USB ports Disable automatic Windows updates Update Windows manually when you do not use...

Page 10: ...109 Yes Photon Counting Assays requiring high sensitivity with an average dynamic range Use when non calibrated data is desired Excellent discrimination between signal and background noise 10 to 25 x...

Page 11: ...tions on page 45 The package contains the instrument plus one or more boxes that contain accessories Illustration Part Number Description SoftMax Pro Software Latest Version SoftMax Pro Software insta...

Page 12: ...microplates 2400 2464 Dust Cover 5000505 CE Declaration of Conformity 5019680 Card User Guide Download 0120 1319 Datasheet Innovative Solutions 0120 1437 Datasheet StackMax Microplate Handling System...

Page 13: ...Power cord 230 V 4200 0900 Injector module Power supply adapter 0310 5238 Waste drain hose 0700 0861 Injector module communication cable 9000 0964 2 Reagent bottles amber 9000 0965 2 Wash bottles natu...

Page 14: ...ration Part Number Description 0310 5705 Tip extractor 5000592 Diagram Assembly Canopy and Tray 0310 5677 Barcode reader Additional Package Contents for Other Instrument Configurations continued For a...

Page 15: ...king results in damage to the instrument The main instrument components are Item Name Description 1 Top cover Cover for base configuration 2 Plate drawer Houses the plate in landscape or portrait orie...

Page 16: ...t open the top of the box 3 Remove the accessory boxes and lift the cardboard and foam packing materials above the instrument 4 Lift the instrument along with the plastic bag up and out of the shippin...

Page 17: ...able Connection for Injector Module 10 Turn the instrument around so that the control panel faces you Ensure no cables run beneath the instrument Leave sufficient space between the back of the instrum...

Page 18: ...njector Ports Base Configuration Instrument When the instrument configuration includes four injectors the instrument has a factory installed injector module on the left side The injector module tubing...

Page 19: ...ment and then raise the top cover up and over the instrument 3 Remove the two hex screws that secure each detector to the instrument chassis The screws are located inside the access holes in the detec...

Page 20: ...tached 6 Lift the filter cover plate off the detector and then remove the filter or silver open ring from inside the detector 7 Insert the new filter into the detector position the filter cover plate...

Page 21: ...nt right of the instrument The instrument control panel has a DRAWER button that enables you to open and close the plate drawer You can also open and close the plate drawer from within the SoftMax Pro...

Page 22: ...the M injector tip assembly The position of the switch is indicated by the size of the circle directly in front of the M injector tip assembly The large circle on the dial of the switch is for 96 well...

Page 23: ...escription of each setting see the SoftMax Pro Software application help Home Tab Simulator Configuration When you operate the instrument in Offline or Simulator mode the Simulator Configuration dialo...

Page 24: ...thod does not require sample preparation Select Custom Multi PMT Normalization Microplate when the instrument has multiple PMTs and you want to use the light spectrum from your own assays to calibrate...

Page 25: ...ll you to insert a 96 well plate into the instrument with material of greater than 100k RLU in wells B2 G2 B11 and G11 After you insert the plate click OK Manual Set This option enables you to use you...

Page 26: ...the data quality to determine whether the seal can stay on the plate for the read To change the plate chamber temperature 1 In the SoftMax Pro Software select the Operations tab and click Temperature...

Page 27: ...ach P injector and each M injector to prime 3 In the of Injections Per Injector field enter the number of 100 L injections to do per prime 4 In the P Inj Prime Speed field enter the desired injector s...

Page 28: ...gs may be reset to the default protocol settings 3 In the Navigation Tree select a Plate section 4 Click in the Settings Information area of the Workspace or select the Home tab and click Acquisition...

Page 29: ...assays with massive dynamic range b If you select Auto Range Analog Only or Max Range in the previous step you must select the Target Calibration Wavelength option that most closely corresponds to th...

Page 30: ...n the Shake Duration field enter the number of seconds to shake the plate between 1 and 999 seconds d In the Shake Speed field enter how fast the plate is to shake in millimeters per second e In the S...

Page 31: ...rcode to overwrite the file name with the barcode 16 After you define the protocol settings click OK to close the Settings dialog 17 Create a template if applicable 18 Set Data Reduction parameters if...

Page 32: ...efore you request service by a service engineer or you return parts to Molecular Devices for repair Molecular Devices does not accept items that have not been decontaminated where applicable to do so...

Page 33: ...al or plastic injector tip The metal tip is recommended unless the assay requires a metal free path 1 Power off the instrument 2 Loosen the captive screws on the front of the instrument and raise the...

Page 34: ...apter out of the port Plastic Insert the pointed end of the tip extractor into the injector port and then pull the adapter out of the port Insert the tip extractor into the injector port a second time...

Page 35: ...te end of the injector tip fits inside the injector adapter Plastic Insert the plastic injector tip into the injector port then insert the injector adapter into the port Assembled Metal Tip and Adapte...

Page 36: ...t tubing you should perform a before and after calibration to ensure consistent results When not in use for long periods of time open the stabilizer lids on top of the pumps to relieve compression on...

Page 37: ...cess Door Opened 3 To remove the tubing from the pump housing pull the retaining stop away from the injector and then disconnect the ends of the tubing from the barbed fittings 4 Loop the portion of t...

Page 38: ...de of the instrument 2 Fill a bottle with filtered deionized water and then place the other ends of the tubing into the bottle CAUTION Do not mix the waste and the input water bottles Doing so can con...

Page 39: ...d select the 96 well Standard plate 8 Select the Read Area category and select the Select All check box 9 Select the Integration Time category and enter an Integration Time of 1 second 10 Select the S...

Page 40: ...ting step 3 below Recommended Life Vol L Displays the fixed value determined by the SoftMax Pro Software Delivered Vol L Displays the volume of fluid injected since the last reset Reset to 0 L Click t...

Page 41: ...te drawer if present CAUTION Always turn off power to the instrument using the power switch Do not shut down power by unplugging the main power cable from the wall outlet Doing so can damage the instr...

Page 42: ...y controlled area For more information about acceptable storage environments see Instrument Specifications on page 45 WARNING LIFTING HAZARD To prevent injury use a minimum of two people to lift the i...

Page 43: ...he instrument 3 Loosen the captive screws on the front of the instrument and then raise the top cover up and over the instrument 4 Turn the plate carriage release knob clockwise until it stops The pla...

Page 44: ...s Obtaining Support Molecular Devices is a leading worldwide manufacturer and distributor of analytical instrumentation software and reagents We are committed to the quality of our products and to ful...

Page 45: ...ates All 96 and 384 well plates with standard SBS footprints 128 2 mm by 86 0 mm and heights from 14 mm to 15 mm Same as base configuration Injectors Base Configuration Multiple Detectors Injectors Ca...

Page 46: ...quipment Classification Group 1 Class A This equipment is designated as scientific equipment for laboratory use that intentionally generate and or use conductively coupled radio frequency energy for i...

Page 47: ...y of Molecular Devices LLC or their respective owners Specifications subject to change without notice Patents www moleculardevices com productpatents FOR RESEARCH USE ONLY NOT FOR USE IN DIAGNOSTIC PR...

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