Molecular Devices SpectraMax 190 Operator'S Manual Download Page 1

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SPECTRAmax

190

Microplate Spectrophotometer
Operator’s Manual

Molecular Devices Corporation

1311 Orleans Drive
Sunnyvale, California 94089

Part # 0112-0085

Rev. A

Summary of Contents for SpectraMax 190

Page 1: ...SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale California 94089 Part 0112 0085 Rev A...

Page 2: ...ed trademarks of Apple Computer Incorporated All other company and product names are trademarks or registered trademarks of their respective owners Disclaimer Molecular Devices Corporation reserves th...

Page 3: ...ut limitation any implied warranty that the products sold under this agreement are merchantable or are fit for any particular purpose except such warranties expressly identified as warranties and set...

Page 4: ...play 1 7 The Microplate Drawer 1 7 The Back Panel 1 8 Functional Description 1 9 Stand Alone Operation 1 9 Wavelength Selection 1 9 Temperature Regulation 1 9 Computer Control 1 10 Specifications 1 12...

Page 5: ...and Cautions 4 3 General 4 4 Cleaning 4 4 Cleaning the Fan Filter 4 4 Changing the Fuses 4 5 Error Codes and Probable Causes 4 7 Error Messages 4 7 Opening the Drawer Manually 4 11 Appendix A Printer...

Page 6: ...5 Figure 1 3 Control Panel 1 5 Figure 1 4 Microplate Drawer 1 7 Figure 1 5 Components on the Back Panel of the SPECTRAmax 190 1 8 Figure 2 1 View of Rear Panel 2 4 Figure 4 1 Power Switch Fuse Box an...

Page 7: ...working with the system Indicates a condition involving potentially infectious bio logical agents requiring that proper handling precautions be taken Glossary of Terms Absorbance A The amount of light...

Page 8: ...viii SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 9: ...thCheck 1 4 Component Description 1 5 Control Panel 1 5 Control Panel Display 1 7 The Microplate Drawer 1 7 The Back Panel 1 8 Functional Description 1 9 Stand Alone Operation 1 9 Wavelength Selection...

Page 10: ...Chapter 1 1 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 11: ...desired and different calculations can be made based on this data using SOFTmax PRO software including the subtraction of blanks use of standard curves etc Typical applications include DNA quantitatio...

Page 12: ...vity properties of compounds in solution Quantitative analyses can be performed on the basis of extinction coef cients without standard curves e g NADH based enzyme assays When using a cuvette the pat...

Page 13: ...l The control panel consists of an LCD and seven pressure sensitive color coded membrane keys which can be used to perform the stand alone func tionality of the SPECTRAmax 190 including setting the te...

Page 14: ...or down arrow key scrolls up or down through the available wavelengths start ing at the previous setting Pressing the up or down arrow once increments or decrements the wavelength shown in the display...

Page 15: ...The middle of the display shows the wavelength The Microplate Drawer Figure 1 4 Microplate Drawer The microplate drawer is located on the right side of the SPECTRAmax 190 and slides in and out of the...

Page 16: ...ker switch labeled I O for on and off respectively Power cord receptacle plug the power cord in here Fuse box cover cannot be opened while the power cord is plugged in When opened it provides access t...

Page 17: ...EMP On Off key will cause the instrument to begin warming the microplate chamber The temperature set point defaults to 37 0 C at start up Accuracy of the temperature set point is guaranteed only if th...

Page 18: ...RAmax 190 SOFTmax PRO makes it possible to access the full capabilities of the SPECTRAmax 190 SOFTmax PRO allows you to Read microplates for endpoint kinetic or spectral scans Use up to six wavelength...

Page 19: ...ray scale data in seven shades of gray corresponding to high and low lim its in a microplate format Kinetic or spectrum plots of all 96 microplate wells Enlarge the display of individual well plots an...

Page 20: ...OD Photometric accuracy linearity microplate 0 2 0 OD 190 850 nm 1 0 and 0 006 OD Photometric precision repeatability 0 2 0 OD 190 850 nm 1 0 and 0 003 OD Stray light 0 05 at 340 nm Photometric stabil...

Page 21: ...bient to 45 C when temperature regulation enabled The ambient temperature must be 20 C to achieve temperature regulation at 45 C Resolution 0 1 C Accuracy 1 0 C Temperature uniformity at equilibrium 0...

Page 22: ...pin DIN RS 232 serial double shielding required Printer interface Parallel 25 pin to Centronics double shielding required Microplates supported All 96 well and strip well microplates including lids E...

Page 23: ...Instrument Description SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual 1 15...

Page 24: ...Chapter 1 1 16 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 25: ...Chapter 2 Installation Installation Warnings 2 3 Installation Cautions 2 3 Unpacking 2 3 Setting Up for Stand Alone Use 2 4...

Page 26: ...Chapter 2 2 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 27: ...carton Please retain the carton and the packing materials If the unit should need to be returned for repair you must use the original packing materials and carton for ship ping If the carton has been...

Page 28: ...e protector between the power cord and the grounded power outlet 5 If you are using a printer for reading microplates in stand alone mode locate the printer port 25 pin parallel on the rear panel of t...

Page 29: ...Chapter 3 Stand Alone Operation Prepare for a Reading 3 3 Turn the Instrument and Printer On 3 3 Set the Temperature 3 3 Select the Wavelength 3 3 Operation Overview 3 3...

Page 30: ...Chapter 3 3 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 31: ...ble the incubator press the incubator TEMP On Off key The LCD display will update to show the temperature set point and the current cham ber temperature To change the temperature set point press the u...

Page 32: ...t wavelength by pressing the up or down arrow near Scroll up or down through the list of wavelengths shown in the LCD using the up or down arrows until the desired measurement wavelength is highlighte...

Page 33: ...roubleshooting Technical Support 4 3 Warnings and Cautions 4 3 General 4 4 Cleaning 4 4 Changing the Fan Filter 4 4 Changing the Fuses 4 5 Error Codes and Probable Causes 4 7 Error Messages 4 7 Openin...

Page 34: ...Chapter 4 4 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 35: ...an be safely performed by quali ed personnel Maintenance not covered in this manual should be performed by a Molecular Devices representa tive Removal of protective covers that are marked with the Hig...

Page 36: ...directed to a tray at the bottom of the instrument from which they will exit to the bench or counter beneath the instrument Wipe up any spills immediately Clean only the exterior of the unit and the m...

Page 37: ...They may be taped to the back of the SPECTRAmax 190 If you no longer have spare fuses you may obtain new ones from Molecular Devices part numbers 4601 0013 for U S 4601 0014 for metric or from a local...

Page 38: ...t you replace both fuses however to ensure continued proper operation Pull both fuses out of the holder and discard them Figure 4 3 The Fuse Box and Holder with Fuses Removed 8 Insert new SLOWBLOW rat...

Page 39: ...is manual are considered fatal if they are detected during power up the instrument will abort the power up sequence and display FATAL ERROR on the LCD panel Check the following table to see if you can...

Page 40: ...ent could not perform command because a measurement was in progress 107 no data to transfer Inputting transfer when there s no data in the buffer 108 data buffer full To many data sets in the buffer C...

Page 41: ...03 unable to cal dark cur rent Too much stray light detected on power up faulty or discon nected pre amp boards 304 signal level saturation 305 reference level saturation 306 plate air cal fail low li...

Page 42: ...find its photo interrupt 402 grating error Grating did not move to its photo interrupt in a reasonable time or can t find its photo interrupt 403 stage error Stage did not move to its photo interrupt...

Page 43: ...wer still remains closed turn the power off and using your thumb nail locate the groove in the upper left side wall of the door Open the door and with your index nger pull the microplate drawer out of...

Page 44: ...Chapter 4 4 12 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 45: ...Appendix A Printers and Cables Compatible Printers A 3 Cables A 3...

Page 46: ...Appendix A A 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 47: ...r obsolescence and the ability of SOFTmax PRO software to print on PC and Macintosh compatible printers Molecular Devices does not recommend reliance on printing in the stand alone mode Cables Molecul...

Page 48: ...Appendix A A 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 49: ...Appendix B Accessories Items Available for Use with the SPECTRAmax 190 B 3 for Instruments with Serial Numbers Beginning with NN...

Page 50: ...Appendix B B 2 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 51: ...0091 Cable RS 232 PC to SPECTRAmax 190 9 pin to 8 pin DIN 9000 0149 Fuse 4 amp 5 20 mm Time Delay 4601 0014 Power Cord US Canada Japan Mexico India 4400 0002 Power Cord EC1 Germany France Scandinavia...

Page 52: ...Appendix B B 4 SPECTRAmax 190 Microplate Spectrophotometer Operator s Manual...

Page 53: ...1 7 1 9 3 3 Installation 2 3 K Keys control panel 1 5 L Labels 1 8 LCD Liquid Crystal Display 1 5 3 4 4 7 M Microplate 1 3 96 well 1 8 filter bottom 1 8 strip wells 1 8 temperature uniformity 1 8 Micr...

Page 54: ...erator s Manual T Technical service 4 3 Temperature 1 9 1 10 3 3 equilibration 1 10 3 3 incubator 1 10 3 3 regulation 1 9 set point 1 6 1 10 3 3 setting 3 3 stabilization 1 10 W Warranty i ii Waveleng...

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