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Millipore Labscale, User Manual

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3.  Turn pump on—increase pump speed until feed pressure 

gauge reads 1.38 Bar (20 psi). 

Note: Check system connections for leaks—tighten connec-

tions if  required 

4.  Adjust retentate valve restriction by slowly turning retentate 

valve clockwise   until the retentate pressure gauge reads 

0.69 Bar (10 psi). 

5.  Adjust pump speed and retentate valve restriction to achieve 

desired feed retentate pressures (recommend 2.07 Bar (30 psi) 

feed / 0.69 Bar (10 psi) retentate). 

RETENTATE TUBING

Figure 34: Concentration Setup. 

Note: Do not exceed 4.14 Bar (60 psi) feed pressure. 

6.  Continue processing until the desired concentration or volume 

is reached. Turn pump off. 

Summary of Contents for Labscale

Page 1: ...Labscale Tangential Flow Filtration System User Guide...

Page 2: ...illipore Corporation Millipore Corporation assumes no responsibility for any errors that may appear in this document This manual is believed to be complete and accurate at the time of publication In n...

Page 3: ...bscale 500 mL Reservoir Setup 16 Labscale Stir Base Set Up 19 Labscale Pump Module Set Up 21 Install Cleaning Tubing 25 Install Cleaning Tubing 25 Clean System 26 Drain System 27 Flush System 29 Flush...

Page 4: ...46 Labscale TFF System Maintenance 47 Labscale Stir Base Spare Parts 47 Labscale 500 mL Reservoir Spare Parts 47 Labscale Pump Module Spare Parts 49 Pump Check Valve Flushing 50 Diaphragm Pump Mainte...

Page 5: ...ore performing maintenance on the system Failure to follow instructions could result in user injury or system damage Read and understand all maintenance instructions in this operating instruction manu...

Page 6: ...pressure product loss damaged cassettes chemical spills and pump damage Carefully follow all operating instructions Prior to each use carefully inspect all hoses and tubing for kinks or restrictions...

Page 7: ...nnected to electrical power and have fuses installed to operate The Labscale system must only be uesd with the Millipore wall transfomer provided and Millipore accessories Location Power Supply Refere...

Page 8: ...in an overpack carton that contains three separate cartons One carton contains the Labscale 500 mL Reservoir one contains the Labscale Stir Base with the Labscale pump module installed one contains t...

Page 9: ...te pressure gauge indicates the pressure of the fluid exiting the Pellicon XL device The upper manifold includes a retentate back pressure valve The fittings used are polypropylene and stainless steel...

Page 10: ...Shut off Valve 10 Fittings 10 Fittings 10 Clamps 1 Syringe 1 Stir Bar 1 Millex Vent 1 Operating Manual 3 Rotating Lock Rings 6 feet 1 8 m of Silicone translucent tubing 1 8 in 3 mm ID 2 x 1 ft 0 3m of...

Page 11: ...nob for the stirrer is located on the front of the stir base 1 Stir Bar 120 V 230V GB 230V 2 Thumbscrews 1 Operating Manual 1 Stir Bar 120 V 230V GB 230V 2 Thumbscrews 1 Operating Manual Figure 4 Labs...

Page 12: ...with the Millipore Labscale Stir Base 4 clamps 4 fittings 1 Installation Instructions 3 feet of Silicone translucent tubing 1 8 3mm ID 1 x 1 ft 0 3m of Sta Pure peristaltic pump tubing 1 8 in 3 mm ID...

Page 13: ...parately The Pellicon XL device includes an Accessories Kit that is packed in the Pellicon XL box below the device The parts in the accessory kit are not required for this system installation User Sup...

Page 14: ...instructions COVER MILLEX VENT FILTER VENT PORT FEED PRESSURE GAUGE RETENTATE PRESSURE GAUGE RESERVOIR SHUT OFF VALVE TANK OUT PORT FEED IN PORT RET OUT PORT PERM 2 OUT PERM 1 OUT RET IN PORT RETENTA...

Page 15: ...ale 500 mL Reservoir set up with Labscale TFF System MILLEX VENT FILTER FEED PRESSURE GAUGE RETENTATE PRESSURE GAUGE ERVOIR T OFF VALVE OUT PORT FEED IN PORT PERM 2 OUT PERM 1 OUT PERMEATE TUBING Figu...

Page 16: ...nslucent Install Retentate Tubing 1 Cut silicone translucent tubing and install fittings as shown in figure 10 L 15 cm 6 inches Fittings 1 male 1 female MALE FEMALE L L Appropriate length to reach MAL...

Page 17: ...bing 1 Cut silicone translucent tubing and install fittings as shown in figure 12 L 15 cm 6 inches Fittings 1 male 1 female MALE FEMALE L L Appropriate length to reach collection vessel Fittings 2 mal...

Page 18: ...tlet port TANK OUT and insert the female luer end of the tank outlet valve over the TANK OUT port Turn the lock nut until snug TANK OUTLET VALVE TANK OUT PORT MILLEX VENT FILTER VENT PORT PERM 2 OUT P...

Page 19: ...1 Turn stirrer and pump if equipped speed controls to the off position 2 Connect power cord to power cord receptacle located at the rear of the system base 3 Align detent on connector with receptacle...

Page 20: ...lugs from the pump inlet and pump outlet ports 2 Turn on the pump speed control set to 2 and listen for pump motor 3 Turn off the pump speed control 4 Turn on the stirrer speed control listen for the...

Page 21: ...the Labscale Stir Base WARNING Disconnect power connection before proceeding 1 Remove the blank panels from the system base as shown in figure 18 2 Install gasket and make wiring connections as shown...

Page 22: ...ite FIELD not used ARM 6 Black ARM 5 Red Pump Control 5 Red 6 Black After prolonged storage the tubing may absorb a small volume of water As a result the tubing color may change from translucent to op...

Page 23: ...n figure 21 Use only one Sta Pure white tubing for pump outlet The other Sta Pure white tubing is used for cleaning Install Pump Inlet and Outlet Tubing 1 Insert one female luer end of the silicone tr...

Page 24: ...ORT FEED IN PORT TANK OUTLET VALVE Figure 22 Install pump inlet and outlet tubing INSTALLATION TIP To reduce pump pulsation increase tubing length 100mm 4 in of additional tubing will only increase fi...

Page 25: ...Figure 23 Setup of cleaning tubing fittings and clamps Remove the plug from the Device Feed port FEED and insert one of the female luer ends of the cleaning tubing over the FEED port Turn the lock nu...

Page 26: ...e Fittings 2 female RETENTATE BACK PRESSURE VALVE VENT PORT WASTE COLLECTION VESSEL TANK OUTLET VALVE CLEANING TUBE DEVICE RETENTATE PORT DEVICE FEED PORT Figure 25 System Cleaning steps 1 6 3 Remove...

Page 27: ...ten connec tions if required 8 Set pump speed to 2 Recirculate for 15 30 minutes Turn pump off RETENTATE TUBING RETENTATE TUBING PUMP OUTLET TUBING COLLECTION Figure 26 Setup for system cleaning steps...

Page 28: ...retentate port Reconnect pump outlet Sta Pure white tubing 4 Disconnect pump outlet Sta Pure white tubing from FEED IN port and place in collection vessel Open tank outlet valve turn pump speed up to...

Page 29: ...Labscale Tangential Flow Filtration System User Guide 29 Flush System Repeat instructions for System Cleaning substituting purified water in place of cleaning solution...

Page 30: ...h Press device firmLy onto reservoir ports Turn the lock nuts until snug PERMEATE TUBING RETENTATE TUBING RESERVOIR FEED PORT RESERVOIR PERM 1 PORT RESERVOIR PERM 2 PORT RESERVOIR RET PORT DEVICE FEED...

Page 31: ...d water Remove plug from VENT port and open tank outlet valve PERMEATE TUBING RETENTATE TUBING RESERVOIR FEED PORT RESERVOIR PERM 1 PORT RESERVOIR PERM 2 PORT RESERVOIR RET PORT DEVICE FEED PORT DEVIC...

Page 32: ...connec tions if required 7 Adjust retentate valve restriction by slowly turning retentate valve clockwise until the retentate pressure gage reads 0 69 Bar 10 psi 8 Adjust pump speed and retentate val...

Page 33: ...anslucent from the tank outlet valve Close the retentate valve Integrity Test Procedure 1 Obtain air integrity test pressure and airflow specifications from the Certificate of Quality supplied with th...

Page 34: ...valve The Pellicon XL device is integral if the measured airflow is lessthan or equal to the airflow specification on the Certificate of Quality Preconditioning 1 Place end of permeate tubing silicon...

Page 35: ...translucent from the permeate outlet PERM 2 port Drain permeate tubing into the waste collection vessel 2 Reconnect the permeate tubing to the PERM 2 port Concentration 1 Remove reservoir cover and fi...

Page 36: ...rning retentate valve clockwise until the retentate pressure gauge reads 0 69 Bar 10 psi 5 Adjust pump speed and retentate valve restriction to achieve desired feed retentate pressures recommend 2 07...

Page 37: ...d now drain by gravity If additional drainage is required a syringe can be placed on the end of the retentate tube and fluid can be blown down Figure 35 Product Recovery Setup 3 Replace retentate tubi...

Page 38: ...up Diafiltration Vacuum Install Diafiltration Tubing 1 Cut silicone translucent tubing and insert a male luer x 1 8 barb port in one end of the tubing as shown here MALE Place the other end of the dia...

Page 39: ...ltration DIA RECIRC port Plug VENT port 3 Insure that the end of the tubing remains below the buffer level at all times As you remove permeate a vacuum is created in the reservoir which will siphon di...

Page 40: ...acked with the device for the most current cleaning solution information Cleaning Agent Concen tration Catalog Nos with prefix PXB Biomax polyether sulphone Catalog Nos with prefix PXC PL regenerated...

Page 41: ...VENT port and either leaving open or installing a Millex Filter 4 Open tank outlet valve 5 Turn pump on increase pump speed until feed pressure gauge reads 1 38 Bar 20 psi Note Check system connectio...

Page 42: ...just retentate valve restriction by slowly turning retentate valve clockwise until the retentate pressure gage reads 0 69 Bar 10 psi Adjust pump speed and retentate valve restriction to achieve 2 07 B...

Page 43: ...Device Repeat flushing procedure detailed in Flushing section page 31 See instructions enclosed with the Pellicon XL device for storage information Clean Base 1 Disconnect power 2 Exterior surfaces o...

Page 44: ...eight 45 7 cm 18 inches Weight 5 9 kg 13 lb Maximum pump outlet pres sure 5 86 Bar 85 psig Maximum gauge pressure do not exceed gauge scale Maximum Pellicon XL Device operating pressure see instructio...

Page 45: ...ne Reservoir cover gasket EPDM Luer fittings 316 stainless steel and polypropylene Tubing Platinum cured silicone translu cent and Sta Pure white tubing Diaphragm pump housing glass filled polypropyle...

Page 46: ...in voltage limits 89 336 CEE relating to Electromagnetic compatibility EMC Standards to which conformity is declared applicable are the following EMC emissions EN61000 3 2 Harmonic currents EN61000 3...

Page 47: ...XX42LSS13 Includes Labscale 500 mL Reservoir Labscale Stir Base with Labscale Pump Module installed Labscale TFF system components are also available separately Description Catalog Number Labscale 50...

Page 48: ...230 Pump head 480 mL min 3 roller XX8000003 Labscale system 1 8 in tubing kit XX80LFL25 After prolonged storage the tubing may absorb a small volume of water As a result the tubing color may change fr...

Page 49: ...P43 5 Transformer 230V Great Britain 1 XX42LSP44 6 Motor Stirrer 1 XX42LSP45 7 Pump Assembly Diaphragm 1 XX42LSP46 8 Speed Controller Stirrer 1 XX42LSP47 9 Speed Controller Pump 1 XX42LSP48 Labscale 5...

Page 50: ...Lok Male x 10 32 St St l 10 XX42LSP27 10 10 1 10 2 Tubing Platinum cured Sta Pure Tubing XX80LFL25 6 ft 2 ft 11 11 1 11 2 11 3 11 4 11 5 11 6 Plastic Fitting Kit Fitting Female Fitting Male Luer Lok P...

Page 51: ...e Pump Module Spare Parts Item Description Qty per Cat Catalog Number 1 Fitting Male Luer Lok x 10 32 St St l 10 XX42LSP27 2 Manifold Pump 1 XX42LSP80 3 3 1 3 2 3 3 Pump Rebuild Kit Contains XX42LSP81...

Page 52: ...silicone translucent from the tank outlet valve 3 Disconnect the pump outlet tubing Sta Pure white from the reservoir FEED IN port and place the end in a waste collection vessel 4 Fill a syringe with...

Page 53: ...mp from the pump manifold 3 Remove the 2 check valves and 4 gaskets from the check valve housing 4 Install new check valves and gaskets as shown below Diaphragm Replacement 1 Unscrew the diaphragm fro...

Page 54: ...D 1 DIAPHRAG M 1 CHECK VALVES 2 G ASKETS 4 PUMP SCREW S 4 FLOW FLOW IMPORTANT Check Valve orientation is as shown Improper orientation of the check valves will damge the check valves and cause the pum...

Page 55: ...Labscale Tangential Flow Filtration System User Guide 55 Electrical Schematic...

Page 56: ...te voltage AC adapter plugged into receptacle on the rear of system enclosure Check fuse on the rear of the system enclosure Pump operates but the stirrer does not Have a qualified technician check th...

Page 57: ...See check valve replacement proce dure in maintenance section Diaphragm Pump Maintenance of this manual Have a qualified technician check the function of the pump speed controller No Flow Pump operati...

Page 58: ...mp inlet or pump outlet tubing par ticulates may be impairing the pump check valves from closing Ensure pump check valves are clean See check valve flushing procedure in maintenance section Pump check...

Page 59: ...Labscale Tangential Flow Filtration System User Guide 59...

Page 60: ...isit the tech service page on our web site at http www millipore com techservice Millipore Pellicon Biomax and Millex are registered trademarks of Millipore Corporation The M logo and Labscale are tra...

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