32 www.millipore.com
6. Reconnect the retentate silicone (translucent) tubing to the
RET IN port and turn pump on - increase pump speed until
feed pressure gauge reads 1.38 Bar (20 psi).
Note: Check system connections for leaks—tighten connec-
tions if required
7. Adjust retentate valve restriction by slowly turning retentate
valve clockwise until the retentate pressure gage reads
0.69 Bar (10 psi).
8. Adjust pump speed and retentate valve restriction to achieve
2.07 Bar (30 psi) feed pressure and 0.69 Bar (10 psi) retentate
pressure.
RETENTATE TUBING
Figure 31: Flushing System Setup.
9. Continue flushing until the level in the reservoir drops to 50
mL. Turn pump off.
