Microm HM 355 S Instruction Manual Download Page 66

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Rotary Microtome HM 355 S

MICROM International GmbH
Robert-Bosch-Str. 49

D- 69190 Walldorf

386 200 - English

Problem

Cause Removal

Cutting drive motor cannot be
started

−

Emergency stop button is

activated.

−

Foot pedal not connected.

−

Deactivate the emergency stop

button.

−

Connect foot pedal or interlock

plug.

Note:

In case of malfunctions and/or service work, please turn off the instrument and contact your
local dealer.

Summary of Contents for HM 355 S

Page 1: ...Rotary Microtome HM 355 S MICROM International GmbH Robert Bosch Str 49 69190 Walldorf 387841 English ROTARY MICROTOME HM 355 S 2 INSTRUCTION MANUAL ...

Page 2: ...Rotary Microtome HM 355 S MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf 387841 English ...

Page 3: ...her warranty is expressed or implied Unauthorized modification or repair by third party persons will void the warranty The warranty will expire in case of improper or wrong use of the instrument and in case the warning and precautionary messages are not observed MICROM International GmbH is not liable for any occurring damage Errors and omissions excepted Subject to amendment and improvement witho...

Page 4: ...es would then be null and void Only skilled or specially trained personnel must operate the microtome i e clamping the specimen trimming and first cuts sectioning and transferring sections onto a slide The listed and marked safety measures as well as the regulations and hygiene measures of your respective lab must strictly be observed MICROM serial number Please check the MICROM Ser No on the type...

Page 5: ...RT 3 OPERATING INSTRUCTIONS 3 1 Setting up the microtome 3 2 Initial turn on 3 2 1 Operating panel 3 3 Display and key functions 3 3 1 Setting the cutting window 3 3 2 Start and stop of the cutting drive 3 3 3 Handwheel brake 3 3 4 Emergency stop 3 3 5 Indication of cutting processes 3 3 5 1 Section counter 3 3 5 2 Section thickness sum 3 3 5 3 Remaining travel to front end position 3 4 Setting se...

Page 6: ...lamps 3 12 Specimen clamping 3 12 1 Universal cassette clamp 3 12 2 Universal cassette clamp adjustable 3 12 3 Standard specimen clamp 3 12 4 Insert for round specimens V insert and V distance piece 3 12 5 Foil clamp 3 12 6 Segment arc and universal specimen holder 3 13 Knife carrier 3 13 1 Disposable blade carrier ER 3 13 2 Disposable blade carrier E 3 13 3 Knife carrier C 3 13 4 Readjusting the ...

Page 7: ...national GmbH Robert Bosch Str 49 D 69190 Walldorf 387841 English PART 6 CONDITIONS FOR THE TRANSPORTATION OF THE INSTRUMENT 6 1 Returning the instrument for repair and routine maintenance 6 2 Disposal of the instrument after final shutdown ...

Page 8: ...14971 2001 03 Medical devices Application of risk management to medical devices ISO 14971 2000 DIN EN 61010 1 2002 08 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements IEC 61010 1 2001 DIN EN 61010 2 101 2003 09 Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements...

Page 9: ...Rotary Microtome HM 355 S MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf 387841 English Hans Heid Walldorf 16 June 2005 Managing Director ...

Page 10: ... visible on the instrument Hazard of hand injuries Due to moving parts in connection also with the microtome knife a danger area arises which might lead to hand injuries in case of non compliance with the safety features of the microtome and when disregarding the instruction manual Biohazard Warning of biological danger Radioactivity Warning of radioactive danger Chemicals Warning of unhealthy or ...

Page 11: ... CAUTION MAINS VOLTAGE Never remove instrument covers during operation Component replacements as well as adjustments must only be made by trained service personnel Only use original spare parts for replacement work Unplug the unit before removing or opening the covers EMERGENCY STOP Cutting movement can be stopped immediately by pressing one of the two emergency stop devices The hand emergency sto...

Page 12: ...Manual 1984 of the World Health Organization When working with infectious specimens observe all applicable safety procedures When working with infectious material appropriate safety and disinfection measures must be carried out According to the rules and regulations concerning the handling of infectious material of the respective laboratory safety clothing e g particle mask gloves protective shoe ...

Page 13: ...hard sectioning techniques in biology medicine and industry Only skilled or specially trained personnel must operate the microtome i e clamping the specimen trimming and first cuts sectioning and transferring sections onto a slide The listed and marked safety measures as well as the regulations and hygiene measures of your respective lab must strictly be observed Note This instruction manual is pa...

Page 14: ...ing stroke The motorized coarse feed system allows the continuous specimen forward and backward travel with variable speed settings This way specimen and knife edge distance can be adjusted quickly The operating panel is placed on the left side of the microtome It can be removed and used separately also on the right side of the instrument For this the operating knobs can be removed and installed o...

Page 15: ...Rotary Microtome HM 355 S MICROM International GmbH Robert Bosch Str 49 D 69190 Walldorf 387841 English Total overview Start Stop Retraction MEMO MENU Fig 1 ...

Page 16: ...TIONAL EQUIPMENT OPTIONAL 2 2 2 1 KNIFE CARRIER Art Nr Standard knife carrier N 705670 Disposable blade carrier E 705640 Disposable blade carrier ER 705650 2 2 2 2 SPECIMEN CLAMPS Standard specimen clamp 715010 Universal cassette clamp 715020 Adjustable universal cassette clamp 716130 Foil clamp 715030 Sandwich supporting material 176010 Insert for round specimens Ø 6 mm 715070 Insert for round sp...

Page 17: ...0 Stereomicroscope Stemi 2000 755210 Adapter for Stemi 2000 532090 KL 1500 with ring illumination 230 V 50 60 Hz 760280 120 V 50 60 Hz 760290 2 2 2 4 MIKROTOMMESSER Steel knives type c 12 cm 152010 16 cm 152020 18 5 cm 152270 22 cm 152030 Steel knives type d 12 cm 152060 16 cm 152070 22 cm 152080 Tungsten carbide knife 16 cm d 152120 Knife cases 12 cm 152220 16 cm 152230 18 5 cm 152280 22 cm 15224...

Page 18: ... English 2 2 2 5 ADDITIONAL ACCESSORIES Fast freezing unit K 34 100 V 50 60 Hz 115 V 60 Hz 230 V 50 Hz 240 V 50 Hz Section transfer system STS 100 V 50 60 Hz 115 V 60 Hz 230 V 50 Hz 240 V 50 Hz Lubricating oil 100 ml 350110 Lubricating oil 250 ml 350120 Paraffin repellent PARA GARD 100 ml 350170 ...

Page 19: ...th reset Remaining travel to front end position 5 digit Specimen size when using a standard specimen clamp max 55 x 50 mm Specimen orientation x and y axes universal 8 Rotation 360 Cutting drive motorized electronically controlled Modes of operation interval single multi and continuous mode Cutting speed 0 430 mm s Coarse feed motorized variable speed Storage temperature range 20 C up to 50 C Oper...

Page 20: ...e influenced by nearby instruments which generate vibrations Handwheel and coarse feed wheel must be free and accessible in a comfortable way Remove the separately packed section waste tray and install it at the base plate from the front side In the rear part of the instrument there are sliding feed for an easy moving of the microtome Slightly lift the base at the front end only and slide the micr...

Page 21: ...can be attached to the instrument or used freestanding First connect the operating panel with the instrument A control cable fig 3 1 is fixed on the rear side of the instrument Connect this cable with the connector on the rear side of the operating panel Should the operating panel be attached to the instrument push the connector through the corresponding hole fig 3 2 on the rear side of the microt...

Page 22: ...drive motor cannot be started Always connect the foot pedal Connect the power cord to the power socket fig 3 4 on the rear side of the instrument Turn on the power switch fig 3 5 Then the specimen holder moves to the rear end position This movement is always carried out when the instrument is turned on This way the instrument is calibrated The insert for the two fuses is placed beside the power sw...

Page 23: ...ert Bosch Str 49 D 69190 Walldorf 387840 Deutsch 3 2 1 OPERATING PANEL The operating elements of the panel are clearly arranged and allow for a safe operation of the instrument The operating panel can be removed from the instrument and used stand alone Fig 5 ...

Page 24: ... length of the cutting window during each further passing through of the cutting window zone A cutting window should only be set while the specimen is moved downwards If by mistake a cutting window limit is set during return travel of the specimen the set limits are applied to the cutting movement accordingly 3 3 2 START AND STOP OF CUTTING DRIVE The cutting drive can be turned on and off by means...

Page 25: ...ion For your personal safety the handwheel brake should be turned on when working on the specimen holder or knife carrier For this press button fig 8 1 First press button fig 8 2 to stop the motorized cutting movement Then activate the function handwheel brake by means of the button fig 8 1 The red LED in the button fig 8 1 lights up Now the motorized cutting drive cannot be started by mistake To ...

Page 26: ...rized cutting drive STOP is shown on the display of the operating panel fig 10 1 if the emergency stop is activated Continue sectioning pull out the red button The cutting drive can be started again The second emergency stop device is integrated into the foot pedal Vigorously step on the foot pedal to immediately stop the motorized cutting drive This emergency stop device is activated as long as t...

Page 27: ...s press button fig 11 1 until the required information is shown on the display If no information is required in this line press button fig 11 1 until this line of the display is blank 3 3 5 1 SECTION COUNTER The section counter fig 12 1 adds up the number of sections produced After each downward movement of the specimen holder the number on the section counter increases by 1 The counter can be res...

Page 28: ...s value shows the distance in microns which is left for sectioning fig 14 3 If the specimen holder is in the back end position the display shows 28 000 µm This number decreases the closer the specimen comes towards the front The respective end position is shown via the flashing LEDs fig 15 3 and 15 4 Fig 14 Fig 15 FINE 9 TRIM 30 REM TRAV 50 R ON 0 3 1 3 4 ...

Page 29: ...erating knob fig 16 1 can also serve to reduce the motorized cutting speed see part Automatic speed reduction 3 9 Note Whenever the instrument is turned on FINE sectioning is automatically selected and shown accordingly FINE pre selected section thickness TRIM pre selected trimming thickness The graduation of the section thicknesses is divided into five ranges Range Graduation up to 5 µm 0 5 µm fr...

Page 30: ... TRIM In the operating mode NORMAL a rim is shown on the display around the TRIM value fig 19 1 When this mode is activated turn the knob to alter the value During each handwheel rotation the specimen holder is moved forwards by the pre selected trimming value in the upper reversal point Note In addition press the knob for the coarse feed fig 18 2 a further TRIM feed can be released even if the FI...

Page 31: ... shown on the display around the FINE value fig 21 1 When this mode is activated turn the knob to alter the value Turn the handwheel in a clockwise direction to feed the specimen at the selected section thickness The same process is carried out when the motor drive for the cutting movement is turned on The handwheel on the right side of the instrument rotates Caution When using the motorized cutti...

Page 32: ...oves down sectioning is carried out cutting movement fig 22 2 Continue turning the handwheel to bring the specimen back up fig 22 4 To protect the knife and specimen during return travel the specimen is retracted R The yellow LED RETRACTION fig 23 1 lights up If desired the function retraction can be turned off The instrument is able to adjust the cutting range to the size of the specimen This is ...

Page 33: ...ontinuously from 0 430 mm s on the control knob fig 25 3 The cutting speed refers to the cutting window For the upward return travel a proportionally higher retraction speed is carried out To adjust the cutting window in relation to a specimen it can be set continuously within the maximum values Note The motorized cutting drive can only be started when the functions handwheel brake fig 24 2 and em...

Page 34: ...s slow Turn the knob further towards the front the speed will be higher To move the specimen holder backwards turn the knob Bild 26 2 backwards i e in the opposite direction of the user Turn angle increase will effect higher return speed In case the knob is kept for more than two seconds at a high speed the specimen movement automatically moves in its rear end position The knob can then be release...

Page 35: ...n fig 30 1 In the submenu Mode select between the four operating modes via the knob fig 30 1 The selected operating mode is shown on the display on the operating panel Press the button fig 28 3 to loosen the handwheel brake Then the cutting movement can be started For this either use the foot pedal or press the button START STOP fig 28 2 on the operating panel During the motorized cutting movement...

Page 36: ...e to release a single cutting cycle For this first loosen the brake via the button fig 28 3 The movement stops in the upper reversal point MULTI STROKE The number or the cutting strokes depends on the respective pre selection 2 up to 99 Press the button fig 28 2 or foot pedal once the multiple cutting cycle is released After the set number of sections has been carried out the movement stops in the...

Page 37: ...a the knob fig 31 1 To start a continuous cutting cycle use the foot pedal or press the START STOP button fig 28 2 For this first loosen the brake via button fig 28 3 To stop the continuous stroke press the foot pedal or the button fig 28 2 once more However if a cutting cycle has been started it will continue through and stop in the next upper reversal point Mode Cont Select Turn knob Confirm Pre...

Page 38: ... date can be set in the instrument Press the menu button fig 35 1 The sub menu fig 36 appears on the display Select Time and date via the turning knob and confirm it by pressing the knob Note The time can be shown constantly in the normal display mode via the scroll button Fig 35 Mode Autom V reduction Display mode Retraction ON OFF Language Time and date Service Menu Fig 36 1 ...

Page 39: ...ws the currently active section thickness as a large number together with a symbol for the fine sectioning range or the trim sectioning range Press the menu button fig 35 1 The sub menu fig 39 appears on the display Select the display mode via the turning knob and confirm it by pressing the knob NORMAL Fig 37 LARGE Fig 38 Mode Autom V reduction Display mode Retraction ON OFF Language Time and date...

Page 40: ...he knob to select the desired function R ON or R OFF fig 40 Press the knob to confirm the setting The yellow LED RETRACTION however might still be on and goes off only after having passed from the return travel again to the cutting movement by means of the handwheel To turn on again the function Retraction please proceed as described above Note The respective functioning state is shown on the disp...

Page 41: ...nglish French Spanish Italian Press the menu button fig 44 1 In the sub menu select Languages via the knob fig 45 1 Turn the knob to select the desired language Press the knob to confirm the selected language Press the menu button fig 44 1 to return to the NORMAL display mode The display fig 43 now shows the terms in the desired language Language ENGLISH Select Turn knob Confirm Press Fig 43 Fig 4...

Page 42: ... sectioning to fine sectioning via knob fig 46 1 results in a reduction of the motorized cutting speed according to the corresponding preselection To preselect the speed reduction press the menu button fig 47 1 and select the sub menu Autom V reduction fig 48 Turn the operating knob fig 46 3 to select the maximum speed in from 10 up to 100 To store the selected speed press the knob fig 46 3 Press ...

Page 43: ...NDLE For safer working with the motorized cutting drive the handwheel handle fig 49 can be pushed in To lock the handwheel handle first take the handle on its outer bush and push it inside until it locks To unlock the handle turn off the motorized cutting drive and press the interior locking knob of the handwheel handle Fig 49 1 2 ...

Page 44: ...o the knife To bring the specimen into the desired position turn the clamping lever fig 50 1 towards the front This will loosen the specimen clamp and a rotation of 360 on the cylinder axis Z axis is possible With the two orienting screws fig 50 2 the specimen clamp can be oriented 8 in each direction on the X axis and Y axis After having oriented the specimen turn the lever fig 50 1 upwards to fi...

Page 45: ...he clamping lever fig 51 1 downwards and pull it sideways Now the specimen clamp can be pulled out to the front and can be replaced with another specimen clamping system Insert the new specimen clamp into the cylinder head so that the clamping lever can be put through the hole of the adapter fig 54 5 from the right side all the way through Then align the specimen clamp by means of the orienting sc...

Page 46: ...that the specimen clamp cannot be clamped The clamping lever fig 52 1 should be in an almost upright position To determine the clamping position of the clamping lever adjust the inner screw fig 53 1 on the backside of the orienting adapter by means of an Allen key size 3 mm Turn the Allen key in a clockwise direction if no clamping was achieved at all or if the clamping position of the clamping le...

Page 47: ...presents a quick change system To insert or remove the cassette from between the fixed fig 54 1 and movable fig 54 2 jaws pull the lever fig 54 3 to the front in the direction of the arrow Note To achieve optimal clamping keep the locating surface of the cassette free of paraffin 3 12 2 UNIVERSAL CASSETTE CLAMP ADJUSTABLE To insert and or remove cassettes between the fixed jaw fig 55 1 and the mov...

Page 48: ... orienting adapter fig 56 6 indicates the four holes for the fastening screws Note For the stability of the specimen do not let it project over the clamping jaws too much The standard specimen clamp is supplied with two movable jaws which are different in weight The lightweight jaw is used in addition with inserts for round specimens The heavier jaw is intended to be used together with the standar...

Page 49: ...ped into the standard specimen clamp The pin fig 57 3 which fits into the lower clamping jaws fig 56 1 positions the insert precisely The two springs fig 57 4 make it easy to remove the specimen from the inserts fig 57 1 and 57 2 To fasten the V distance piece fig 57 C on the fixed jaw fig 56 2 of the standard specimen clamp the clamping screw fig 56 3 must be unscrewed from the spindle Pull the s...

Page 50: ...ver its position can be changed Turn the three clamping screws fig 58 5 to clamp the specimen tightly When using an orienting adapter with the instrument first insert the enclosed graduated ring into the orienting adapter by means of the pin With the graduated ring the orientation in X Y direction is annulled However it is possible to turn in Z axis 60 in either direction Then insert the foil clam...

Page 51: ...and clamped by means of the screw fig 59 2 with the hex head wrench fig 59 3 Then the holder together with the specimen is put into the segment carrier fig 59 5 where the holder can be turned 360 Thus the specimen can be aligned as required Then the holder is clamped by means of the screw fig 59 4 in the selected position The carrier fig 59 5 can be moved on the base fig 59 6 In this way the surfa...

Page 52: ... blades Insert the blade into the slot behind the clamping plate fig 60 5 For this turn the clamping lever fig 60 7 towards the front Swing the bracket with scale fig 60 4 to the front Loosen the clamping lever fig 60 7 and if necessary slightly push the lower part of the clamping plate fig 60 5 as well Insert the blade on the rail fig 60 3 and push it from the side to the middle Afterwards return...

Page 53: ...espective requirements of the tissue to be sectioned Loosen the clamping lever fig 60 8 on the right side of the knife carrier and move the upper part fig 60 9 of the knife carrier on the base fig 60 1 The adjusted clearance angle can be read on the scale Then turn the clamping lever fig 60 8 upwards to lock in the new clearance angle The clamping lever for the angle adjustment can be pulled off a...

Page 54: ... as well A small gap between rail fig 61 3 and clamping plate fig 61 5 can be seen Insert the blade on the rail fig 61 3 and push it from the side to the middle Afterwards return the clamping lever fig 61 7 upright thus locking the blade in position The bracket fig 61 4 is provided with a scale After loosening the clamping lever fig 61 7 and after having moved the bracket fig 61 4 upwards move the...

Page 55: ...experience usable cuts are only achieved at a clearance angle of 10 or more If the clamping lever fig 61 3 is loosened the upper part of the knife carrier can be moved 1 cm to the left or right side This way the cutting edge can optimally be used The clamping lever for the angle adjustment can be pulled off after a correct angle setting to avoid that the angle is reset unintendedly Moving the knif...

Page 56: ...Clearance angle adjustment The clearance angle between cutting edge and specimen can be shifted and adjusted to the requirements of the tissue to be sectioned Loosen the clamping lever fig 62 4 on the right side of the knife carrier and move the upper part of the knife carrier fig 62 2 on the base fig 62 1 The adjusted clearance angle can be read on the side scale Then turn the clamping lever fig ...

Page 57: ...uipped with two knife guards fig 62 7 which can be moved sideways These knife guards should be pushed together in the middle while adjusting knife or specimen This reduces the danger of injury considerably Central clamping plate The knife is clamped and stabilized in the cutting zone by the central clamping plate fig 62 8 exactly where the highest cutting forces are applied Two types of clamping p...

Page 58: ...ow a sufficient clamping the clamping can be adjusted as follows For this loosen the set screw fig 65 1 on the lower side of the knife carrier Insert a pin into one of the holes of the brass screw fig 65 2 and turn the brass screw When the clamping is to be strengthened turn the brass screw fig 65 2 to the right side When the clamping is to be loosened turn the brass screw to the left side Before ...

Page 59: ... 200 English 3 14 SECTION WASTE TRAY WITH INTEGRATED ARM REST The section waste tray with integrated arm rest can easily be cleaned The section waste tray surrounds the knife carrier area and allows non tiring working with the microtome It can be easily removed to the front at any time Fig 66 ...

Page 60: ...y shown on the display Then proceed with the routine work trimming first cuts fine sectioning When the work on the block has been finished briefly press the MEMO button The specimen clamping then moves backwards Unclamp the specimen and insert a new specimen The specimen surface is now in the first cut position Warning The stored cutting position can only be used effectively when blocks with the s...

Page 61: ...e instrument hood Remove the front plastic caps from the upper side of the housing If there are no drilled holes in the housing it must be replaced with a new one with drilled holes Fasten the peg fig 68 4 to the plate fig 68 6 with the screw fig 68 7 Carefully introduce the four sleeves fig 68 8 into the drilled holes with the screws fig 68 5 Warning Please note that the sleeves do not fall into ...

Page 62: ... working with the sharp area of the cutting edge or have the knife resharpened For optimal sectioning front and back of the knife must be clean Especially paraffin waste must be removed thoroughly Setting the cutting angle Inclination of the knife in relation to the block surface Each microtome knife has a so called facet cut resulting from the fact that when producing a knife or when resharpening...

Page 63: ...the knife might vibrate too much while sectioning The so called chatters appear on the block surface as well as on the sections These chatters are parallel stripes in relation to the knife edge having negative effects on the microscopical evaluation of sections Between these two limit cases the correct knife inclination must be determined A clearance angle between facet angle and block surface of ...

Page 64: ...ar sources of heat Tightening the clamping screws Tighten all clamping screws and clamping levers on the knife carrier specimen holder and specimen orientation Selection of the knife Carefully select the required knife material and profile Adjustment of the knife Take care to adjust the proper clearance angle of the knife 5 7 for glass knives For diamond knives ask the knife manufacturer Select a ...

Page 65: ...e can be found Turn handwheel slower Chatter on sections Cutting speed too high Unfavorable clearance angle Insufficient clamping on specimen clamping and or knife carrier Turn handwheel slower Try clearance angle adjustments until an optimal angle can be found Check all screw and clamping connections on specimen clamping and knife carrier Tighten them if necessary No feed is carried out anymore a...

Page 66: ...h Problem Cause Removal Cutting drive motor cannot be started Emergency stop button is activated Foot pedal not connected Deactivate the emergency stop button Connect foot pedal or interlock plug Note In case of malfunctions and or service work please turn off the instrument and contact your local dealer ...

Page 67: ...he left side of the knife carrier and pull the knife carrier towards the front Clean the operating controls and the surfaces of the knife carrier especially the space where the knife carrier is installed Clean the consoles handwheel coarse feed wheel specimen clamping system specimen orientation as well as the base plate and housing Note Mild domestic cleaners can be used to clean the microtome Do...

Page 68: ...NANCE Annual routine maintenance To secure section quality and functioning of the microtome it is recommended that a routine maintenance be performed by a trained service technician once a year Service contract MICROM offers a service contract which guarantees that your instrument is always in perfect condition For more information please contact the nearest MICROM sales office Note We strongly re...

Page 69: ...ng of fuses For power requirements 220 240 V 2 fuses 1 6 AT slow blow For power requirements 100 120 V 2 fuses 3 2 AT slow blow Replacing the fuse for the motor supply The fuse for the motor supply fig 73 4 is placed on the rear side of the instrument on the right side of the mains plug unit and secures the supply voltage of the cutting drive To replace the two fuses turn off the power switch of t...

Page 70: ...entioned measures for transportation preparations In addition the conditions for storage and transportation as mentioned in part 2 3 must be observed during the entire transportation Biohazard Please also note the precautionary measures described in our safety precautions concerning biological hazards Measures for taking out of operation Turn off the main switch of the instrument Remove knife or b...

Page 71: ...ation 6 2 DISPOSAL OF THE INSTRUMENT AFTER FINAL SHUTDOWN After the final shutdown of the instrument we recommend to contact a local recycling company for the disposal according to the national applicable regulations To be applied in the countries of the European Union and other European countries with a separate collecting system within the waste management The marking of the product and or the r...

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