9
MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
3
Storage conditions and preparation of working
solutions
Attention:
Buffers RCU contains chaotropic salt. Wear gloves and goggles!
CAUTION: Buffer RCU contains guanidinium thiocyanate which can form highly reactive
compounds when combined with bleach (sodium hypochlorite). DO NOT add bleach or acidic
solutions directly to the sample-preparation waste.
• All kit components should be stored at room temperature (18–25 °C) and are stable up
to one year. Storage at lower temperatures may cause precipitation of salts.
• Check that 96–100 % ethanol is available as additional solution in the lab.
Before starting any
NucleoSpin
®
RNA Clean-up
protocol, prepare the following:
• Clean-up Buffer RCU:
Add the indicated volume of 96–100 % ethanol to the Clean-up
Buffer RCU Concentrate. See table below or bottle label for necessary volumes. Store
Buffer RCU at room temperature (18–25 °C) for up to one year.
• Wash Buffer RA3:
Add the indicated volume of 96–100 % ethanol (see table below) to
Wash Buffer RA3 Concentrate. Mark the label of the bottle to indicate that ethanol was
added. Store Wash Buffer RA3 at room temperature (18–25 °C) for up to one year.
NucleoSpin
®
RNA Clean-up
REF
10 preps
740903.10
50 preps
740903.50
250 preps
740903.250
Clean-up Buffer
RCU (Concentrate)
5 mL
Add 15 mL ethanol
5 mL
Add 15 mL ethanol
5 x 5 mL
Add 15 mL ethanol to
each bottle
Wash Buffer RA3
(Concentrate)
6 mL
Add 24 mL ethanol
12 mL
Add 48 mL ethanol
50 mL
Add 200 mL ethanol
