Macherey-Nagel NucleoSpin RNA Clean-up XS, User Manual

Page: 6 / 19

7 MACHEREY-NAGEL – 03/2021, Rev. 05
RNA clean up XS
Table 1: Kit specifications at a glance
Parameter NucleoSpin
®
RNA Clean-up XS
Technology Silica membrane technology
Format Mini spin columns – XS design
Sample material < 300 µL RNA solution containing < 90 µg RNA
Fragment size > 200 nt
Typical recovery 85–95 %
A
260
/A
280
1.9–2.1
Elution volume 5–30 µL
Preparation time Approx. 20 min/6 preps
Binding capacity 110 µg
2.3 Handling, preparation, and storage of starting materials
RNA intended to be used as sample for the
NucleoSpin ® RNA Clean-up XS
procedure
should be handled with the same care as any RNA sample. The stability of prepurified
RNA samples (e.g., RNA isolated with phenol based protocols) depends very much on the
performed procedure.
Wear gloves at all times during the preparation. Change gloves frequently.