MACHEREY-NAGEL – 03/2021, Rev. 05
12
NucleoSpin
®
RNA Clean-up XS
4
Wash and dry silica membrane
1
st
wash
Add
400 µL Buffer RA3
to the NucleoSpin
®
RNA XS
Column. Centrifuge for
30 s
at
11,000 x
g
. Discard
flowthrough and place the column back into the
Collection Tube.
+ 400 µL RA3
11,000 x
g
,
30 s
+ 200 µL RA3
11,000 x
g
,
2 min
2
nd
wash
Add
200 µL Buffer RA3
to the NucleoSpin
®
RNA XS
Column. Centrifuge for
2 min
at
11,000 x
g
to dry the
membrane. Place the column into a nuclease-free
Collection Tube (1.5 mL, supplied).
If for any reason, the liquid level in the Collection Tube
has reached the NucleoSpin
®
RNA XS Column after
centrifugation, discard flowthrough and centrifuge again.
5
Elute RNA
Elute the RNA in
10 µL RNase-free H
2
O
, (supplied) and
centrifuge at
11,000 x
g
. for
30 s
.
If higher RNA concentrations or higher elution volumes
are desired, elution volume may be varied in the range
of 5–30 µL.
For further details on alternative elution procedures see
section 2.4.
+ 10 μL
RNase-free
H2O
11,000 x
g
,
30 s
