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User Manual

www.logosbio.com

LBSM-SM-ML-LU7-001
VL2005-02

Summary of Contents for L70001

Page 1: ...0 User Manual www logosbio com LBSM SM ML LU7 001 VL2005 02...

Page 2: ...boratory instrument for scientific research use only It is not a medical therapeutic or in vitro diagnostics device Do not disassemble the device on any occasion as this will invalidate your warranty...

Page 3: ...oduct Description 7 LUNA FX7 Automated Cell Counter 7 LUNA FX7 Cell Counting Slides 8 2 GETTING STARTED 8 Installation 8 LUNA FX7 startup 9 Screen Saver 9 3 COUNTING CELLS 10 Sample Preparation 10 Sam...

Page 4: ...PROCESS FEATURE 21 Bioprocess Feature 21 Creating a bioprocess protocol 21 Record bioprocess data 21 Review Export bioprocess data 22 Graph 22 7 QUALITY CONTROL 23 Quality control mode 23 Validation s...

Page 5: ...e 36 Powering on off 36 Cleaning 36 Troubleshooting 36 Inaccurate cell count 36 Data transfer and saving 37 Software update errors 37 11 PRODUCT SPECIFICATIONS 37 LUNA FX7 Automated Cell Counter 37 LU...

Page 6: ...moving the instrument Disconnect the power cord in the case of abnormalities Do not hold the slide slot while it is in motion Protect USB drives from being infected with viruses and malware Operating...

Page 7: ...ted Cell counter 1 Power Cord with AC Adapter 1 Cell Counting Slides Sample 2 ea 4 slide types Trypan Blue Stain 0 4 2 x 1 mL Acridine Orange Propidium Iodide Stain 2 x 0 5 mL LUNA FX Calibration Bead...

Page 8: ...t The automated slide port enables one time slide insertion Power button The power button is used for the main power control USB ports USB ports allow the user to transfer or print cell count data Dat...

Page 9: ...me 5 1 L 1 3 L chamber 1 3 L chamber 0 5 L chamber 2 Getting Started Installation Installation Place the LUNA FX7 on a clean level and sturdy surface o Avoid vibrations from other devices o Do not ins...

Page 10: ...used to count cells and calculate the viability of cells stained with fluorescence dyes Acridine Orange Propidium Iodide AO PI F23001 Cell lines Primary cell mode may also be used to count cells expre...

Page 11: ...ple Loading Load the appropriate volume for each slide chamber according to the table below LUNA 1 Channel Slides LUNA Cell Counting Slides PhotonSlide LUNA 3 Channel Slides LUNA 8 Channel Slides 50 L...

Page 12: ...ed channels Photobleaching will occur with procolonged exposure so work appropriately Important Adjusting light intensity levels in the COUNT screen will only be applied to the live view mode Exposure...

Page 13: ...t the image is in focus for the first field of view When the first field is in focus press the COUNT button The LUNA FX7 will count all slide chambers as designated in SETTINGS Counting time will vary...

Page 14: ...concentration or number can be graphed according to cell size Cell size gating parameters may be changed by pressing the slider rectangles An active slider will be highlighted in blue Move the slider...

Page 15: ...ed image TIF Tagged images of cells Raw image TIF Untagged images of cells Report PDF Report with data images and histograms Bioprocess Onboard graph CSV Growth curve displayed onboard Growth rate dou...

Page 16: ...x designation The appended suffix may be a sequential number or the date time Default Quick save preferences may be pre set in SETTINGS SAVE REVIEW 4 Review Reviewing Data Review images Press REVIEW N...

Page 17: ...eate the desired protocol Important During reanalysis changing exposure levels in the protocol will have no effect on the results of the reanalyzed counting data Press REVIEW and select a folder from...

Page 18: ...imal results for most cell types but protocols for specific cell types or applications may need to be optimized Creating protocols To create a new protocol select the DEFAULT protocol and press SAVE A...

Page 19: ...creen Protocol Parameters Brightfield cell counting parameters Parameters for Brightfield cell counting Total cell counting Parameter Range DEFAULT Min search size m 1 89 7 Max search size m 2 90 24 C...

Page 20: ...positive calls Live cell sensitivity Live cells with intact cell membranes exclude trypan blue and erythrosin B The dyes stain the cytoplasm of dead cells with compromised membranes As a result object...

Page 21: ...these parameters to adjust the minimum and maximum cell sizes to be included in results The base unit is 1 micrometer GF RF threshold level Green and Red fluorescence threshold will determine the lev...

Page 22: ...L and create a new protocol Edit and save the new protocol as needed Record bioprocess data 1 To record bioprocess data go to SETTINGS within a counting mode Set Counting chamber area is set to Curren...

Page 23: ...PORT TO USB 5 To delete selected bioprocessing data press ERASE ALL The data but not the protocol will be deleted Graph Press GRAPH to view charted results Press the Y axis title box to alternate betw...

Page 24: ...only be used in conjunction with the Logos Biosystems fluorescence or brightfield validation slides Validation slides contain a pre spotted pattern brightfield or pre fixed beads fluorescence of known...

Page 25: ...he product key is included with the product information of the validation slide Contact sales logosbio com if the product key is lost or missing Important The validation slides have a limited lifetime...

Page 26: ...Assay value differs from what was provided with the validation slide check to ensure the product key was entered correctly If entered correctly contact sales logosbio com Set Acceptance limits Accept...

Page 27: ...tered control in the REGISTER main page and pressing Load Performing Quality Control Navigate to Quality Control mode and press REGISTER Select a validation slide from the list of Registered controls...

Page 28: ...acceptance limits established during slide registration Press RESULTS to see the counting data If the results are not within the acceptance range redo RUN QC steps If not met again contact your local...

Page 29: ...iew a graphical representation Press the Y axis title box to switch between Concentration Total cell counts and Viability Press Day Month or Year to alter X axis scale Press EXPORT to export a CSV fil...

Page 30: ...the 3 Ch slide option is selected results of all chambers may be combined If Combine results of selected chambers is ON the Counting chamber is automatically set to all When combining results the PDF...

Page 31: ...automatically add sequential numbers to the prefix name OR select Date Time to automatically append date and time to the prefix name o Next name Displays file name to be used for the next count to be...

Page 32: ...ct an Ethernet cable to the instrument When connected an IP address will appear on the screen in blue color WiFi connection Insert the supplied WiFi dongle to a LUNA FX7 USB port Press WiFi Select app...

Page 33: ...changed Server address IP address of the server hosting the CountWire software Ask network administrator for details Server port Port number of the server hosting CountWire software Ask network admin...

Page 34: ...Calibration Step 1 window will appear 3 Remove any counting slide from slide port 4 Press START 5 The Calibration Step 2 window will appear 6 Mix one part stain with an equal volume of distilled water...

Page 35: ...Software Logos Biosystems continually provides software updates to ensure optimal performance The current software version is displayed in SETTINGS SOFTWARE 1 The most recent version may be downloade...

Page 36: ...een Open the FileZilla FTP Client on the computer To connect enter the following information o Host IP address being used for the LUNA FX7 o Username logosbio o Password logosbio Click Quickconnect Fo...

Page 37: ...excessive force or pressure as this can damage the touchscreen Troubleshooting Inaccurate cell count Clumped cells Gently but thoroughly pipette your cell suspension to break up aggregates prior to c...

Page 38: ...drive Delete previous versions of software from the USB drive before downloading new software Incompatible USB drive Some USB devices are undetectable or incompatible Use the USB drive supplied with t...

Page 39: ...ation Instruments Cat Product Quantity L70001 LUNA FX7 Automated Cell Counter 1 L70002 LUNA FX7 Automated Cell Counter Bioprocess Package 1 Slides and Reagents Cat Product Quantity L72011 LUNA 1 Chann...

Page 40: ...Stain 2 x 1 mL F23001 Acridine Orange Propidium Iodide Stain 2 x 0 5 mL F23011 Acridine Orange Propidium Iodide Stain Sterile filtered 2 x 0 5 mL F73101 LUNA FX Calibration Beads Kit 2 x 0 5 mL Softw...

Page 41: ...rchase price of the Instrument to the Purchaser if the Instrument is in original conditions or after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period wi...

Page 42: ...nstrument to and from the service facility and if necessary the travel cost of a service engineer after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period...

Page 43: ...31 360 4277 Email info logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 571 266 3925 Email info usa logosbio com EUROPE 11B avenu...

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