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33

 

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3.6 Using the Dilution Calculator   

 

3.6.1  The  LUNA

™  provides a  built-in  dilution calculator that  helps  easily  calculate adjustments to 

obtain a desired concentration. 

 

<Built-in Dilution Calculator> 

 

 

 

3.6.2 The dilution calculator initially shows the current concentration. Put the appropriate numbers 

into the blanks of the “Desired Concentration” and “Final Volume” that you want to obtain. 

 

 

 

Users  can  choose  one  of  “Total”,  “Live”  or 

“Dead” cells for calculating their dilution. 

By  clicking  this  button,  the  “Current 

Concentration” displays the concentration of the 

total, live or dead cells. 

Before  calculating  the  dilution  factor,  please 

check this option to match your requirements. 

Summary of Contents for L10001

Page 1: ...Catalog L10001 Document LBSM MD ML LUB 001 Version VL1712 01...

Page 2: ...oduct Specifications 11 1 4 Product Description 12 Chapter 2 Setting up 15 2 1 Installation 15 2 2 Start Up Screen 16 2 3 Setting 17 2 4 Setting the Date and Time 18 2 5 Save Options 18 2 6 Counting O...

Page 3: ...4 3 Previous Count Button 42 4 4 Setting up the Protocol for Counting 44 Chapter 5 Maintenance and Troubleshooting 47 5 1 Cleaning 47 5 2 Changing the Battery 48 5 3 Calibrating the Counter 49 5 4 Upd...

Page 4: ...urn off the instrument before disconnecting the power cord and or moving the instrument 6 Ensure that the power cord is firmly plugged into the power inlet the wall outlet and AC adapter 7 When the in...

Page 5: ...waste ordinances of your region for proper disposal provisions The CE mark indicates that this instrument conforms to all applicable European Community provisions for which this marking is required U...

Page 6: ...s requirements 3 Hold the LUNA Cell Counting Slide by the edges to avoid touching its optical surface Make sure that no damage or contamination occurs on the optical surfaces of the slide 4 After mixi...

Page 7: ...ment Conditions Operating Power 100 240 VAC 1 5A Frequency 50 60 Hz Electrical Input 12 VDC 3 5A Installation Site Indoor use only Operating Temperature 10 35 Maximum Relative Humidity 20 80 Altitude...

Page 8: ...and the results of cell count and viability will be displayed on the screen The counting image can be downloaded onto a USB drive in TIF format for future analysis The LUNA Automated Cell Counter pro...

Page 9: ...th T bond technology without using hazardous organic solvents Cell concentration viability range Measurements can be made for cells at concentrations within the 1 x 104 1 x 107 cells mL range and for...

Page 10: ...Trypan Blue Stain 0 4 2 x 1 mL USB Drive 16 GB 1 When you receive the product package please immediately check it with the above component list to ensure that all parts are included and no damage has...

Page 11: ...Image Type TIF Optimized for LUNA only Software LUNA software www logosbio com Reporting PDF format report Processing Time Approx 7 seconds at 1 x 106 cell mL concentration Depending on cell type and...

Page 12: ...all functions and displays results 1 4 2 Rear view of the LUNA Automated Cell Counter The rear view of the LUNA Automated Cell Counter shows a power button to turn on or off the instrument and a power...

Page 13: ...bright centers and dead cells with dark centers The slide port is used to insert a compatible slide loaded with the sample into the instrument 1 4 4 Left side view of the LUNA Automated Cell Counter...

Page 14: ...abeled as A and B which can be used for the same sample as duplicates or for 2 different samples The depth of the counting chamber is 100 m The counted cell volume is about 0 5 l almost the same as co...

Page 15: ...NA Automated Cell Counter on a flat stable surface 2 1 3 Insert one end of the power cord into the instrument and plug the other end of the power cord into an electrical outlet after checking the outl...

Page 16: ...setting Start Up Screen of the LUNA Automated Cell Counter Note While operating the instrument the buttons that are not in use are inactivated Note The inner red box in the display screen above is th...

Page 17: ...ight corner of the counting screen Then the following Settings screen appears From here users can change or adjust various preferences or options Settings 2 3 3 The Settings menu allows you to perform...

Page 18: ...using the Backspace button set the date or time using the number buttons and press the Apply button to save the changes 2 5 Save Options 2 5 1 The Save Options screen will be seen as below after sele...

Page 19: ...g counting results and histograms IMPORTANT Remember that the Raw Image MUST be turned on to save the Raw Image which should be provided to your distributor or Logos Biosystems to get the best technic...

Page 20: ...io This option can generate cell viability data For samples with Trypan Blue Stain the dilution factor must be set to value 2 Please check Without Trypan Blue When samples do not contain Trypan Blue d...

Page 21: ...a dye the Dilution Factor must be set to 1 Custom staining In this option cell samples can be stained with Erythrosin B Stain or other dyes or even the culture media for counting Before choosing this...

Page 22: ...djusted automatically even though background of sample is a little brighter or darker Note The LUNA Automated Cell Counter basically analyze results based on image so the instrument can provide an acc...

Page 23: ...e dead cells Dilution Calculator Current Concentration The default value is Total Cells on In order to use the live cells in the Dilution Calculator please turn the live cells on Printer Print Protoco...

Page 24: ...Blue option is provided in the protocol menu section 2 6 2 to meet mostly the total cell counting for some special users it is NOT recommended to count cells without Trypan Blue staining Low contrast...

Page 25: ...Make sure that the counting slide is not inserted upside down Note After this step the real image of the cell sample will be displayed on the screen The cell image can be navigated by touching the sc...

Page 26: ...2x image appears This 2x image may be the best zoom in status for focusing When pressed again a 4x image will be shown Pressing the button again takes the screen back to a 1x image 3 3 3 Press the Cou...

Page 27: ...er to review the data to determine the accuracy of the counting on site without depending on a computer 3 4 2 When touching the Tag button the image on the screen will show objects surrounded by green...

Page 28: ...al buttons in the menu Count Graph Gating 1 Total Live or Dead button 2 Cluster Map button 3 Cell number or Cell concentration button The above buttons can be used in combination and the details are d...

Page 29: ...bination Dead Cells and Cell Number The combination of Dead and Cell number shows the distribution of cell size vs cell number for only dead cells Note When the Cell Number button is pressed it toggle...

Page 30: ...bination Dead Cells and Cell Number The combination of Dead and Cell number shows the distribution of cell size vs cell number for only dead cells Note When the Cell Number button is pressed it toggle...

Page 31: ...oncentration for total cells Combination Live Cells and Concentration The combination of Live vs Cell concentration shows the distribution of cell size vs cell concentration for only live cells Combin...

Page 32: ...ells Live cells Dead cells 3 5 2 Cluster Map button 3 5 2 1 When the Cluster Map button is pressed the percentage distribution of the clustered cells are displayed 1 cell means single cell and 2 cell...

Page 33: ...or initially shows the current concentration Put the appropriate numbers into the blanks of the Desired Concentration and Final Volume that you want to obtain Users can choose one of Total Live or Dea...

Page 34: ...34 www logosbio com Users can also calculate their custom dilution 3 6 3 Press the Calculate button and then a dilution instruction will appear in the message box...

Page 35: ...ive cells are tagged in green and dead cells are tagged in red for further analysis If not checked users can not use the Review function with the saved image So please make sure that this must be chec...

Page 36: ...rt into the USB drive Now the saved files can be opened after transferring them via USB drive to a PC with appropriate software Two page PDF report generated by LUNA 1st page Data and Results Cell cou...

Page 37: ...atically saved shows more detailed cell information such as each cell size and cell status In the csv file the information of each cell can be checked by cell id given by x axis and y axis The resolut...

Page 38: ...ogos Biosystems or your local distributor Note For more information on the LUNA Printer please see the User Manual of the LUNA Printer IMPORTANT Since the LUNA has only one USB port users can use only...

Page 39: ...39 www logosbio com Print Button 3 8 4 The counting results and its protocol will be printed out as below Printed Results Note The Print button prints only the latest counting result...

Page 40: ...ogosbio com Note If the Protocol part of the print is not necessary users can turn off the option in the Results Printer Calculator Option in the Settings menu as shown below Result Printer Calculator...

Page 41: ...menu located at the top of the screen A list of files on the USB drive is displayed on the left side of the screen as shown below Review Screen Note In the USB drive there will be 2 types of image fi...

Page 42: ...Count Button The button Previous Count in the Review menu shows a new window below it that contains up to 1 000 previous counting results that have been saved in the internal memory of LUNA Previous C...

Page 43: ...bio com 1 Export to USB CSV Users can save the data of all previous counts into a USB memory drive which can be handled in PC software 2 Erase all All data can be removed from the memory 3 Close Close...

Page 44: ...t box Note Initially there are two protocols named as DEFAULT and New protocol in the instrument To generate a customized protocol select New protocol first and edit parameters then save as another na...

Page 45: ...ng Noise Reduction 0 10 5 This refers to the decrease of the background for counting Higher noise reduction will not detect faint signals and weakly stained objects Lower noise reduction means increas...

Page 46: ...tton Arrow buttons located over and under the parameter will be activated Press the arrow buttons to change the values for each parameter When no protocol is selected this button is inactivated If you...

Page 47: ...y the cell counter with a dry cloth Do not wet the instrument by pouring or spraying water or other liquids directly on the instrument In particular power related parts should never become wet in orde...

Page 48: ...However if the date time changes or slows with unknown causes it may indicate that the battery is weakening or running out To change the battery contact the service personnel of the instrument suppli...

Page 49: ...is for non Trypan Blue or PBS The counting slide must be removed from the instrument to do this step Step 2 This is for Trypan Blue dye Please insert a slide containing Trypan Blue dye 1 1 diluted wi...

Page 50: ...Calibration Step 1 a new pop up box will come on the screen as shown below Calibration Step 2 2 This step is for calibration with Trypan Blue dye Prepare a calibration sample by mixing 10 l Trypan Bl...

Page 51: ...e on the screen as shown below Calibration Step 3 Note This Cal Step 3 is necessary if you want to use another staining dye for your specific purpose For normal counting it is not necessary so that yo...

Page 52: ...o com 4 Then press the Start button 5 Then you will see the progress bar as below for custom staining Calibration Step 3 6 After Calibration Step 3 you will see the message box as shown below Calibrat...

Page 53: ...ust be saved into the root directory of the USB drive 5 4 3 Insert the USB drive into the USB port on the instrument Then press the Update Firmware button 5 4 4 When the display asks you to update pre...

Page 54: ...rted a soft click can be heard Sample loading If the counting slide is over or under loaded with the sample it may affect counting results The optimal amount of sample is 10 12 l of cell suspension Ma...

Page 55: ...e supplied with the instrument Or make sure that your USB drive is compatible with the instrument The version of the USB drive must be 2 0 Some types of USB drives are not detected or compatible with...

Page 56: ...Counting Slides 500 Slides 10 boxes L12003 LUNA Cell Counting Slides 1000 Slides 20 boxes L12008 LUNA Reusable Slide 1 unit L12010 LUNA Reusable Slide Coverslips 10 units B13101 LUNA Standard Beads 2...

Page 57: ...Biosystems Inc Company will not claim any consideration against the purchaser of infringement of patents owned or controlled by the Company which cover the product based on the manufacture use or sale...

Page 58: ...ion as unintended or unexpected errors including occasional typographies or other kinds are inevitable In addition the Company reserves the right to make any changes necessary without notice as part o...

Page 59: ...185 Fax 82 31 478 4184 Email sales logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 517 266 3925 Email sales logosbio com EUROPE 1...

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