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Focus sharply.
Move the focus slider in the desired direction or enter the desired value in the Z-position box.
Alternatively, click Find Focus to have the CELENA® X find the optimal focal plane. Set the
range to scan from the current focal position. A long search range is useful when finding the
focal plane of an unknown object. A short search range is useful for fine focusing.
The speed of the image-based autofocus is entirely dependent on the set exposure. Reducing
the exposure (< 10 ms) will increase focusing speed.
Set up Multiscan AF.
Click Multiscan AF to set up autofocusing for demanding batch image acquisitions such as
multi-well plate imaging, slide scanning, and time-lapse imaging. Select how often to
autofocus during an automated scan. Select whether to use the image-based or laser
autofocus.
