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Purging Procedure
Before starting a cooling experiment, you will need to purge air from the stage chamber with dry nitrogen.
This will remove the water in the air which would otherwise condense and freeze on the sample disrupting
your image quality.
Before you can start purging, the LNP95 must be set to manual mode.
You can either use the LinkPad touch screen or Linksys32 software.
Use LinkPad to set the LNP95 to
Manual Mode
Touch the active area (1) under ‘Lnp Speed’ to
change to the LNP Screen Menu. Touch
’Man’ (2) to switch the LNP95 to manual mode.
The word ‘auto’ is changed to ’speed’ to indicate
the LNP95 is functioning in manual mode.
Using the Keypad type in 100 (max pump speed)
and touch the ’Enter’ button (4) to start the
LNP95 at the programmed speed (5).
Filling the Liquid Nitrogen Dewar
Please follow your health and safety manual for
directions on how to handle liquid nitrogen and
ensure that you have the correct safety equipment
including gloves and safety goggles.
Fill the Dewar until approximately 2/3 full and
replace the lid with siphon attached.
DO NOT FASTEN THE CATCHES.
Wait for the nitrogen to stop bubbling before
fastening the catches.
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