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Lighthouse ActiveCount100 Operating Manual 

 

248083444-1 Rev 2                                     

 

 

 

P a g e  

39  of  40 

Appendix B:  Feller Conversion Table 

 

Sampling Head with 300 Holes x 0.6mm 

 

R = number of Colony Forming Units (CFU) counted on 90 mm petri dish agar after incubation 

 

P

R

 = Most Probable Number (MPN) of microorganisms in volume of air sampled 

 

N = number of holes in ActiveCount100 sampling head (300) 

 

P

R

 

P

R

 

P

R

 

P

R

 

P

R

 

P

R

 

51 

56 

101 

123 

151 

209 

201 

332 

251 

541 

52 

57 

102 

124 

152 

211 

202 

335 

252 

547 

53 

58 

103 

126 

153 

213 

203 

338 

253 

553 

54 

59 

104 

127 

154 

216 

204 

341 

254 

560 

55 

61 

105 

129 

155 

218 

205 

344 

255 

566 

56 

62 

106 

131 

156 

220 

206 

347 

256 

573 

57 

63 

107 

132 

157 

222 

207 

350 

257 

580 

58 

64 

108 

134 

158 

224 

208 

353 

258 

587 

59 

66 

109 

135 

159 

226 

209 

357 

259 

594 

10 

10 

60 

67 

110 

137 

160 

228 

210 

360 

260 

601 

11 

11 

61 

68 

111 

138 

161 

230 

211 

363 

261 

609 

12 

12 

62 

69 

112 

140 

162 

232 

212 

367 

262 

616 

13 

13 

63 

71 

113 

142 

163 

235 

213 

370 

263 

624 

14 

14 

64 

72 

114 

143 

164 

237 

214 

374 

264 

632 

15 

15 

65 

73 

115 

145 

165 

239 

215 

377 

265 

641 

16 

16 

66 

74 

116 

146 

166 

241 

216 

381 

266 

649 

17 

17 

67 

76 

117 

148 

167 

243 

217 

384 

267 

658 

18 

19 

68 

77 

118 

150 

168 

246 

218 

388 

268 

667 

19 

20 

69 

78 

119 

151 

169 

248 

219 

391 

269 

677 

20 

21 

70 

80 

120 

153 

170 

250 

220 

395 

270 

686 

21 

22 

71 

81 

121 

155 

171 

253 

221 

399 

271 

696 

22 

23 

72 

82 

122 

156 

172 

255 

222 

403 

272 

707 

23 

24 

73 

83 

123 

158 

173 

257 

223 

407 

273 

717 

24 

25 

74 

85 

124 

160 

174 

260 

224 

410 

274 

728 

25 

26 

75 

86 

125 

161 

175 

262 

225 

414 

275 

740 

26 

27 

76 

87 

126 

163 

176 

264 

226 

418 

276 

752 

27 

28 

77 

89 

127 

165 

177 

267 

227 

422 

277 

765 

28 

29 

78 

90 

128 

167 

178 

269 

228 

427 

278 

778 

29 

30 

79 

92 

129 

168 

179 

272 

229 

431 

279 

791 

30 

32 

80 

93 

130 

170 

180 

274 

230 

435 

280 

805 

31 

33 

81 

94 

131 

172 

181 

277 

231 

439 

281 

820 

32 

34 

82 

96 

132 

174 

182 

279 

232 

444 

282 

836 

33 

35 

83 

97 

133 

175 

183 

282 

233 

448 

283 

853 

34 

36 

84 

98 

134 

177 

184 

284 

234 

452 

284 

871 

35 

37 

85 

100 

135 

179 

185 

287 

235 

457 

285 

889 

36 

38 

86 

101 

136 

181 

186 

289 

236 

462 

286 

909 

37 

39 

87 

103 

137 

183 

187 

292 

237 

466 

287 

931 

38 

41 

88 

104 

138 

184 

188 

295 

238 

471 

288 

954 

39 

42 

89 

105 

139 

186 

189 

297 

239 

476 

289 

979 

40 

43 

90 

107 

140 

188 

190 

300 

240 

481 

290 

1006 

41 

44 

91 

108 

141 

190 

191 

303 

241 

486 

291 

1036 

42 

45 

92 

110 

142 

192 

192 

306 

242 

491 

292 

1069 

43 

46 

93 

111 

143 

194 

193 

308 

243 

496 

293 

1107 

44 

47 

94 

113 

144 

196 

194 

311 

244 

501 

294 

1150 

45 

49 

95 

114 

145 

198 

195 

314 

245 

507 

295 

1200 

46 

50 

96 

115 

146 

200 

196 

317 

246 

512 

296 

1260 

47 

51 

97 

117 

147 

202 

197 

320 

247 

518 

297 

1335 

48 

52 

98 

118 

148 

203 

198 

323 

248 

523 

298 

1435 

49 

53 

99 

120 

149 

205 

199 

326 

249 

529 

299 

1585 

50 

55 

100 

121 

150 

207 

200 

329 

250 

535 

300 

1885 

NOTE:  Table values calculated from P

R

 = N [1/N + 1/(N-1) + 1/(N-2) + 1/(N-R+1)]     (Feller, 1950) 

Summary of Contents for ActiveCount100

Page 1: ...ActiveCount100 M I C R O B I O L O G I C A L S A M P L E R Operators Manual 00 10 00 79 02 26 2015 LOC001 12 45 30 PM 0 0 L 00 10 00 USER001 1000L...

Page 2: ...Blank Page...

Page 3: ...ion except as permitted in writing by Lighthouse Worldwide Solutions The information contained herein is subject to change without notice Lighthouse Worldwide Solutions is not responsible for any dama...

Page 4: ...lume 20 Select Total Sampling Volume 20 Active Volume Applies to Sampling Runs 20 Locations and Users 21 Assigns Active Location to Sampling Runs 21 Assigns Active User to Sampling Runs 22 Data Loggin...

Page 5: ...es in Comma Delimited Files 36 Location Database 36 User Database 36 Sampling Run Databases 37 Appendix B Feller Conversion Table 39 Sampling Head with 300 Holes x 0 6mm 39 Appendix C Limited Warranty...

Page 6: ...010 1 2010 CAN CSA C22 2 No 1010 1 1992 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements EMC EN61326 1 2006 Electrical Equipment for...

Page 7: ...wn method impaction to aspirate air through a perforated plate and deposit airborne bacteria onto an agar coated petri dish The petri dish is then removed incubated and tested for live microorganisms...

Page 8: ...finger on the touch screen Power on ActiveCount100H and continue to hold your finger on the touch screen until the Performing touch screen calibration screen appears 3 Follow the prompts to calibrate...

Page 9: ...ling head dust cover Autoclavable sampling head and dust cover Aluminum sampling head Stainless steel sampling head optional Uses standard ISO 24998 90mm petri dishes Status LED Allen wrench to adjust...

Page 10: ...of 40 Plug In USB Flash Drive and Charge Battery Data logging Insert the included USB flash drive to store sampling run metadata Charge battery Follow the instructions below to charge the ActiveCount...

Page 11: ...Operating Manual 248083444 1 Rev 2 P a g e 11 of 40 Get Ready For Sampling Follow these steps to prepare Activecount100 for microorganism sampling When ActiveCount100 is not in use always cover sampl...

Page 12: ...ble number is a well established method to estimate the number of viable microorganisms in a monitored area To derive MPN ActiveCount100 uses the principle that statistically assumes an increasing num...

Page 13: ...me of air Periodic samples periodically for a user specified time and volume Sampling Mode Description Constant ActiveCount100 continuously samples until it reaches the desired sample volume at 100 li...

Page 14: ...minute Periodic mode is useful when monitoring a process over longer periods e g manufacturing a batch or lot of food cosmetics or drugs In the example below select ten cycles over two hours and 1 000...

Page 15: ...URATION button Select Constant in Mode menu item then select total sampling volume If desired also select an optional delay time in seconds Press HOME button to return to the Home screen Begin samplin...

Page 16: ...ATION button Select Periodic in Mode menu item then select total sampling volume delay time total time to sample and the number of cycles Press HOME button to return to the Home screen Begin sampling...

Page 17: ...ecurity Configuration Lock ActiveCount100 Sampling Modes can be restricted by locking the Configuration Screen Before locking the configuration screen select the sample mode and sample settings to be...

Page 18: ...describes ActiveCount100 LED status Status Color Blink Idle Off Off Sampling Blue Solid Delay Pause Green Solid Flow Error Red Blinking Writing to USB White Solid Service Calibration Required Yellow S...

Page 19: ...manual abort by user or c flow error a Success b Manual abort by user c Flow error Sampling Run Metadata Display Sampling Results To display metadata results for the most recent sampling run press ME...

Page 20: ...n the Volume menu item ActiveCount100 displays eight sampling volumes ActiveCount100 indicates the active sampling volume with a dashed line Active Volume Applies to Sampling Runs ActiveCount100 uses...

Page 21: ...igns Active Location to Sampling Runs Locations Database ActiveCount100 stores up to 400 locations in its Locations database When ActiveCount100 completes a sampling run it saves the active location i...

Page 22: ...2 of 40 Assigns Active User to Sampling Runs Users Database ActiveCount100 stores up to 50 users When ActiveCount100 completes a sampling run it saves the active user in a metadata record for the most...

Page 23: ...ying metadata for the most recent sampling run see Section Display Sampling Metadata USB Flash Drive When a USB flash drive is plugged into the rear USB port ActiveCount100 displays a USB flash drive...

Page 24: ...ndicators Status Indicators ActiveCount100 displays status indicators on the Home screen If ActiveCount100 displays Device status flag it may indicate a device issue or problem To display more device...

Page 25: ...ntact information Device status flag Possible device error e g flow error calibration due etc To display the device status press SETTINGS button and Status menu item The sampling database stores these...

Page 26: ...Lighthouse ActiveCount100 Operating Manual 248083444 1 Rev 2 P a g e 26 of 40 Settings ActiveCount100 Settings Device Settings Press SETTINGS button to display and edit device settings...

Page 27: ...zontal Sampling Vertical Sampling Place ActiveCount100 on a horizontal surface to sample vertically Horizontal Sampling Mount ActiveCount100 on a tripod monopod and turn the instrument to sample horiz...

Page 28: ...of 40 Optional Compressed Gas Sampler Verify Nominal Flow Before Gas Sampling Before using the optional compressed gas sampler first verify nominal flow of ActiveCount100 without the gas sampler Add e...

Page 29: ...l 248083444 1 Rev 2 P a g e 29 of 40 To verify ActiveCount100 nominal flow rate follow the steps below When ActiveCount100 displays the following screen position gas sampler snugly in the sampling hea...

Page 30: ...oints straight up for nominal flow ActiveCount100 and the Gas Sampler adapter are now ready to sample compressed gas Press the BACK button to configure your gas sample Do not sample explosive nor corr...

Page 31: ...issue Wipe dry with another sterile tissue Always full remove and wipe dry the disinfectant solution before reusing ActiveCount100 Let dry at room temperature Sampling Head Dust Cover Remove sampling...

Page 32: ...ock turn off and disconnect power adapter during sterilization Do not submerge ActiveCount100 in any liquids Do not spray disinfectant solution directly into ActiveCount100 enclosure Do not use gases...

Page 33: ...v 2 P a g e 33 of 40 Calibration Calibration Contact Authorized Facility ActiveCount100 is factory calibrated to 100 liters min 4 Contact a factory authorized facility to calibrate ActiveCount100 annu...

Page 34: ...ogrammable cycles delay sample duration and volume Body made from stainless steel Sampling head made from anodized aluminum Optional sampling head made from polished stainless steel Autoclavable sampl...

Page 35: ...inless steel sampling head Optional gas sampler Conforming Specifications CE 21 CFR Part 11 ISO 14698 1 Biocontamination Control Cleanrooms Associated Control Environments ISO 14698 2 Biocontamination...

Page 36: ...0 0 None Good Good None 08 05 2015 15 02 56 LOC014 6 0 10 0 0 0 0 None Good Good None 08 05 2015 15 04 57 LOC015 6 0 10 0 0 0 0 None Good Good None 08 05 2015 15 05 19 LOC016 6 0 10 0 0 0 0 None Good...

Page 37: ...ted fields for each sampling run CSV Field Description Sample Value 1 Timestamp Date format set in Settings menu US MM DD YYYY EU DD MM YYYY ISO YYYY MM DD Time local 24 hour time HH MM 7 16 2015 9 48...

Page 38: ...USB flash drive not detected Error Error 16 Model Name ActiveCount100 model name ActiveCount100 17 Serial Number ActiveCount100 serial number 0123456789 18 Flow Rate ActiveCount100 flow rate 100 L m 1...

Page 39: ...220 395 270 686 21 22 71 81 121 155 171 253 221 399 271 696 22 23 72 82 122 156 172 255 222 403 272 707 23 24 73 83 123 158 173 257 223 407 273 717 24 25 74 85 124 160 174 260 224 410 274 728 25 26 75...

Page 40: ...act or otherwise all warranties and remedies granted under this Section may at LWS s option be terminated C THE FOREGOING WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER REPRESENTATIONS WARRANTIES AND...

Page 41: ...Service and Support Tel 1 800 945 5905 USA Toll Free Tel 1 541 770 5905 Outside of USA techsupport golighthouse com www golighthouse com...

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