Leica TL5000 Ergo, User Manual

Page: 22 / 54

Leica TL5000 Ergo User Manual 22
The Different Contrast Methods
Bright field
Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
Operation for bright field illumination is explai-
ned starting on page 26 .
Dark Field
For dark field illumination the specimen is lit by
two LED elements opposite from each other so
that only light that has hit the specimen travels
up the beam path of the stereomicroscope. The
structure of opaque objects disperses the light,
making the object visible against a dark back-
ground.
Operation for dark field illumination is explai-
ned starting on page 29 .
Rottermann contrast
Part of the lighting unit is covered depending
on the diaphragm position, which results in
contrast effects. Phase structures typically act
as spatial, relief-type images – in the positive
relief contrast like hills, in the inverted relief
contrast like valleys.
Operation for Rottermann contrast is explained
starting on page 27 .
Fly with bright field illumination Identical subject with dark field illumination Identical subject with Rottermann contrast