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Leica TL5000 Ergo, User Manual

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Leica TL5000 Ergo 

User Manual 

20

1 

Power switch for the base

2 Power 

connection

3 

1 × USB port

4 

3 × CAN bus port

1 

LED display for opening and positioning the 
aperture or for balance in DF mode.

2 

Control for the size of the aperture/balance

3 

"BF" button for bright field (press and hold 
for 2 seconds to (de)activate the automatic 
aperture)

4 

"RC" button for Rottermann Contrast / in the 
RC mode, press and hold the button to define 
the aperture size

5 

"DF" button for dark field 

1 

On/Off switch for the light source / Press and 
hold for 5 seconds to reset the base to factory 
default settings

2 

Controls for controlling the brightness inten-
sity

Back panel

Left side

Right side

An Overview of the Leica TL5000 Ergo

1

3 4 5

2

1

2

1

2

3

4

Summary of Contents for TL5000 Ergo

Page 1: ...Living up to Life User Manual Leica TL5000 Ergo Transmitted Light Base ...

Page 2: ...ica TL5000 Ergo can be used in clean rooms without any problems Cleaning Å You can find notes on cleaning in the appendix which begins on page 53 Servicing Å Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Responsibilities of person in charge of instrument Å Ensure that the Leica stereomicroscope is operated...

Page 3: ...ndicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Å Hazards to personnel Å Functional disturbances or damaged instruments Important information This symbol indicates additional infor mation or explanations that are intended to provide clarity Explanatory notes Å This symbol within the text stands for addi tional information a...

Page 4: ...ncept booklet The instruments and accessories described in this User Manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the inst rument is altered modified or used in conjunc tion with non Leica components that are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights...

Page 5: ...nd observe this User Manual and particularly the safety instructions Repairs service work Å Refer to Safety Concept booklet Å Only original Leica Microsystems spare parts may be used Å Before opening the instruments switch off the power and unplug the power cable Touching the live circuit can cause injury Integration in third party products Å Refer to Safety Concept booklet Disposal Å Refer to Saf...

Page 6: ...cence applications 30 Switching the automatic control system for bright field on and off 31 Resetting the base to factory default settings 32 Preparation for the automatic control system The basics 34 Detection with SmartTouch 35 Detection with Leica Application Suite 36 Detection with Leica Application Suite AF 37 Operation with Leica SmartTouch Leica SmartTouch expands your possibilities 39 An o...

Page 7: ...Leica TL5000 Ergo User Manual 7 Leica TL5000 Ergo ...

Page 8: ...ents of the Leica M series If for example you work with an encoded Leica M205 C stereomicroscope the information on magnifica tion or objectives in use is automatically relayed to your LeicaTL5000 Ergo transmitted light base or Leica SmartTouch control unit Though the reliability and robustness of Leica devices is world renowned the Leica TL5000 Ergo requires a certain degree of care and attention...

Page 9: ...thing is Relative The Leica M series provides maximum flexibility in choosing equipment thanks primarily to the modular configuration and the compatibility that Leica has painstakingly maintained for decades The optics carriers eyepieces bases and more can be combined in any way you choose allowing you to create the microscope that best suits your needs Despite this you will notice that the contro...

Page 10: ...lready been assembled and commissioned by your Leica consultant skip the installation in structions and start with the Quick Start Guide on page 19 If on the other hand you are putting the base and microscope together yourself continue with the Assembly chapter which begins on page 11 ...

Page 11: ...Leica TL5000 Ergo User Manual 11 Assembly ...

Page 12: ... Leica TL5000 Ergo trans mitted light base together with the Leica M205 C stereomicroscope However the transmitted light base can be operated with all Leica M seriesstereomicroscopes withorwithoutfluo rescence For details on assembling the optics carrier tube and eyepieces please consult your stereomicroscope s user manual ...

Page 13: ...5000 Ergo Tools used Å Hex socket screwdriver 3 mm Assembling the column adapter 1 Securely install the column adapter on the column using the four included screws Assembling the focusing column 2 Securely screw the focusing column to the Leica TL5000 Ergo using the 6 included screws Assembling the focusing column ...

Page 14: ...e maximum travel path is changed Exception If the power fails while the moto rized focus is moving the position data are lost In this case the calibration must be repeated using the Leica LAS software or the Leica SmartTouch To do so please consult the respective manual Restricting the bottom travel range 1 Move the motorized focus into the lowest position you want to reach 2 Unscrew the screw of ...

Page 15: ...ty and adaptation of other models please contact your Leica consultant Assembling the Optics Carrier 1 Place the microscope carrier on the focu sing column so that the screw fits into the thread provided and the lug fits into the groove 2 Press the microscope carrier backwards to the focusing column and screw it in place using your other hand 3 Insert the optics carrier into the microscope carrier...

Page 16: ... area of the field of view Preparations 1 Unscrew the positioning screw and remove the protective cover Assembling the tube 2 Push the tube for example the inclined binocular tube into the dovetail ring and rotate it slightly in both directions until the positioning screw meshes with the guide groove 3 While holding the tube only slightly care fully tighten the positioning screw It is automaticall...

Page 17: ...ailable 10 16 25 and 40 wide field eyepieces for persons wearing glasses Preparation 1 If you want to use an optional reticule insert it now 2 Remove the plastic tube guard Inserting the eyepieces 3 Push the eyepieces into the tubes as far as they will go and check to ensure that they fit tightly and accurately 4 Securely tighten the clamping screws ...

Page 18: ... disassembly so that it does not fall onto the stage plate This applies particularly to the 2x planapochromatic objective which is very heavy Remove all specimens from the stage plate first Attaching the objective 1 Remove the protective cover on the optics carrier 2 Screw the objective clockwise into the optics carrier ...

Page 19: ...Leica TL5000 Ergo User Manual 19 Quick Start Guide ...

Page 20: ... field press and hold for 2 seconds to de activate the automatic aperture 4 RC button for Rottermann Contrast in the RC mode press and hold the button to define the aperture size 5 DF button for dark field 1 On Off switch for the light source Press and hold for 5 seconds to reset the base to factory default settings 2 Controls for controlling the brightness inten sity Back panel Left side Right si...

Page 21: ...e respective opening of the aperture influences the area of illumination as well as the light guide A large aperture leads to scattered soft light on the other hand a smal ler aperture leads to a defined intensive light source If the Leica TL5000 Ergo is used with an encoded stereomicroscope the aperture closes automatically based on the selected magnification For unencoded microscopes the apertur...

Page 22: ...imen travels up the beam path of the stereomicroscope The structure of opaque objects disperses the light making the object visible against a dark back ground Operation for dark field illumination is explai ned starting on page 29 Rottermann contrast Part of the lighting unit is covered depending on the diaphragm position which results in contrast effects Phase structures typically act as spatial ...

Page 23: ...ouch control unit The aperture is auto matically adjusted to the respective magnifica tion if this changes the aperture adapts to the new situation automatically in order to provide ideal illumination Encoded stereomicroscopes t FJDB t FJDB t FJDB t FJDB t FJDB Unencoded systems The stereomicroscope is not encoded and cannot relay information about magnification or optics to the base Control takes...

Page 24: ... LED illumination can be very bright Check and adjust the intensity of the illumination to a suitable brightness before looking through the eyepieces Switching the transmitted light base on and off 1 Switch on the transmitted light base with the power switch on the rear side 2 Press the on off switch on the right side once to turn on the illumination Continued on next page Switching the transmitte...

Page 25: ...oned in the remaining part of this User Manual 3 Look through the eyepiece and adjust the light intensity using the control on the right side The intensity of the illumination is visualized by the LED scale 4 Press the on off switch on the right side once again to turn off the illumination on the base Switching the transmitted light base on and off cont d ...

Page 26: ...n natural colors on a bright surface Activating bright field illumination 1 Switch on the base as described on page 24 2 Press the BF Bright Field button once to activate bright field mode For encoded systems the aperture closes or opens the illumination aperture corre sponding to the magnification selected on the stereomicroscope and the optics being used 3 Look through the eyepieces and adjust t...

Page 27: ...he inverted relief contrast like valleys Activating Rottermann contrast 1 Switch on the base as described on page 24 2 Press the RC Rottermann Contrast button once to activate Rottermann cont rast mode and to bring the aperture into position The aperture opens or closes itself corre sponding to the magnification selected on the stereomicroscope and the optics being used 3 Look through the eyepiece...

Page 28: ...rture display The current position of the aperture is visualized by an LED strip on the left side of the base Write down the position of the aperture so that the setting can be reproduced later Modifying the opening of the aperture manually The automatically set opening of the aperture can be reduced manually if the situation requires 1 Press and hold the RC button and simul taneously select the d...

Page 29: ...e of opaque objects disperses the light making the object visible against a dark back ground Activating dark field illumination 1 Switch on the base as described on page 24 2 Press the DF Dark Field button once to activate dark field mode 3 Look through the eyepieces and adjust the light intensity using the control on the right side of the base 4 Change the angle of incidence of the light using th...

Page 30: ...d illumination you can remove the protective glass plate from the light source This way you will have a low reflection background that will therefore be even darker The protective glass plate is located beneath the glass cover of the base directly above the light source Dark Field for Fluorescence Applications ...

Page 31: ...em 1 Enable bright field mode BF 2 Press and hold the BF button for 2 seconds Activating the automatic control system 1 Press the BF button again for 2 seconds to reactivate the aperture s automatic control system Manual control The aperture can be adjusted by the user with the knob on the left side while manual control is active This will for example increase contrast with a reduction in the aper...

Page 32: ... light base can be returned to its default state that is all settings are reset to the value they were upon delivery 1 Hold the on off switch on the right side of the base 5 seconds long in order to return to the factory settings Resetting the Base to Factory Default Settings ...

Page 33: ...Leica TL5000 Ergo User Manual 33 Preparation for the Automatic Control System ...

Page 34: ...equipment configuration have been changed The basics Before the automatic contrast of the Leica TL5000 Ergo base can be used the individual components in the equipment configuration must be detected This is the only way for example that an optimum aperture size can be calculated to eliminate stray light during bright field illumination The factors that need to be detected are Objective visual tube...

Page 35: ...ipment 2 On the Leica SmartTouch touch the Config tab 3 Touch the Config field 4 In the Microscope field select the instru ment categories you are using in sequence 5 In each category touch the component you are using Detection is complete Now all of the components of your stereomicroscope are integrated into the automatic aperture control system correctly ...

Page 36: ... Suite 4 Select the Configure command from the main menu 5 Click the Microscope tab 6 Select the instrument categories you are using in sequence 7 In each category click on the component you are using Detection is complete Now all of the components of your stereomicroscope are integrated into the automatic aperture control system correctly Detection with Leica Application Suite ...

Page 37: ...stereomicroscope are integrated into the automatic aperture control system correctly 4 Select the instrument categories you are using in sequence Detection with Leica Application Suite AF Detection with Leica Application Suite AF 1 Start Leica AF Hardware Configurator 2 Configure your microscope in Hardware Setup 3 Click on the Configure button ...

Page 38: ...Leica TL5000 Ergo User Manual 38 Operation with Leica SmartTouch ...

Page 39: ...uch Controlling the Leica TL5000 Ergo While interacting with the Leica TL5000 Ergo transmitted light base the Leica SmartTouch controls the intensity of illumination the cont rast method as well as the opening and posi tion of the aperture But above all you can save settings and restore them later at the touch of a button This way you obtain reproducible conditions and consequently have maximum as...

Page 40: ...Leica TL5000 Ergo User Manual 40 An Overview of the Leica SmartTouch Touchscreen Toggle button Double rotary actuator Top rotary actuator Bottom rotary actuator Hardware keys freely configurable ...

Page 41: ...The screenshots in this manual may thus differ from the display on your Leica SmartTouch Color coding conventions All fixed elements of the user interface such as labels or titles are displayed in a black font All the values that can be modified such as the zoom factor status of the illumina tor etc are displayed in a red font Controls on the Touchscreen Tab Button Display Display of the current f...

Page 42: ...tab 2 Touch the symbol for the transmitted light base 3 Select the desired type of illumination 4 Put in the desired settings as they are outli ned on the following pages The indicator on the display varies depending on the type of illumination selected Accessing the Leica TL5000 Ergo ...

Page 43: ...ntrol system 1 2 3 4 5 7 1 Reduce Increase brightness by tapping repeatedly 2 Manually open or close the base s aperture by tapping repeatedly 3 Switch on off the aperture s Automatic Control System 4 Select the desired type of illumination 5 Display for the current brightness and aperture settings 6 Switch on off the base s light source 7 Reset button for restoring the default settings for all or...

Page 44: ... light base 3 Touch the RC button 4 Put in the desired settings Rottermann contrast control system 1 3 2 4 5 7 1 Reduce Increase brightness 2 Manually open close the aperture 3 Select a type of illumination 4 Display for the current brightness aperture balance 5 Switch on off the light source 6 Move the aperture backwards forwards with repeated taps in order to control contrast 7 Reset button for ...

Page 45: ...ings Dark field illumination control system 1 3 2 4 5 1 Reduce Increase brightness 2 Move the illumination focal point backwards forwards with repeated taps in order to control contrast 3 Select a type of illumination 4 Display for the current brightness aperture balance 5 Switch on off the light source 6 Reset button for restoring the default settings for all or individual contrast methods ...

Page 46: ...e Brightness symbol 4 Switch on off the LED display on the Leica TL5000 Ergo with the Zoom display button Switching off the LED display The brightness of the LED display can hinder or even prevent observation of light sensitive specimens and during fluore scence applications In such cases the display can be shut off through the Leica SmartTouch ...

Page 47: ...way the standard settings are selectively restored for every or just individual contrast methods Resetting settings 1 Touch the symbol for restoring the default settings 2 Select whether all settings All or only those for the currently active contrast method Current should be reset Resetting settings ...

Page 48: ...n the right side of the base via the blin king of the green LED for the illumination This is the case for example if the LED lighting unit switches itself off due to too high of tempera tures This is due to a safety function designed to prevent damage to the base As soon as the LED lighting unit has cooled off the base can be used again as usual ...

Page 49: ...Leica TL5000 Ergo User Manual 49 Dimensions and Specifications ...

Page 50: ...Leica TL5000 Ergo User Manual 50 Dimensions 292 217 346 70 154 154 431 547 46 70 346 412 Leica M205 C with coarse fine pinion and Leica TL5000 Ergo transmitted light base ...

Page 51: ...rea Dark field 40 mm Connections Relief Contrast System RC Yes CAN 3 Internal shutter lamp control Yes USB 1 Remote control options Yes Power Supply 1 Integrated filter holder Yes Matching of high num aperture Yes Anti Shock Pads Yes Size W H D in mm 412 341 46 Accessories IsoPro cross stage support Integrated heating stage optional Yes Integrated slide adapter optional Yes ErgoRest optional Techn...

Page 52: ...Leica TL5000 Ergo User Manual 52 Appendix ...

Page 53: ...resulting from careless or improper handling Contact address However if your instrument should no longer function properly contact your technician your Leica representative or Leica Microsystems Schweiz AG CH 9435 Heerbrugg E mail contact stereo service leica microsystems com Care Å Protect your equipment from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chem...

Page 54: ...nts Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsu itable cleaning agents and techniques can damage polymers Permitted measures Å Clean the microscope or parts of it using warm soapy water then wipe using distilled water Å For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow...

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