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CONTROLLED COPY 

 Centre for Biological Engineering 

 

Standard Operating Procedure    

 

 

 

 

 

 

SOP194 

 

Title:  Use and maintenance of Leica RM2125 RTS rotary microtome.

 

 

Location: 

H27 

 

 

Version 001 

Effective Date: 24.05.2021 

Review 24.05.2023 

 

Written by: Sotiria Toumpaniari 

 

Reviewed by:  

Approved by: C.Kavanagh 

 

 

Page 10 of 11 

 

  Have the instrument checked at least once a year by a qualified service technician authorized 

by Leica. At the end of the warranty period, enter into a service contract. For more information, 

please contact your local Leica technical service center. 

 Clean the instrument every day. 

  

 Once a month, lubricate the following parts with the included oil 

No. 405 (1-2 drops are enough):  

 The moveable parts of the specimen holder fixture (10) and the 

dovetail receptacle (Fig. 14). 

 T-piece (19) on the microtome base plate. 

 Clamping levers (2) and (20) on the microtome. 

 Guide rails (91) for the knife holder base on the microtome base 

plate. 

 The clamping lever (39) and (24) on the right and left on the knife 

holder base. 

 The guide (92) of the lateral movement on the knife holder base 

(21). 

 The T-piece (26) on the knife holder base (21). 

 The sliding surfaces of the knife guard (3) and knurled nuts (57) 

on knife holder N. 

 The clamping lever (89) on knife holder E. 

 Shaft (90) of the clamping lever of the cassette clamp (see Fig. 

36). 

 

  Special Safety Note:

 Equipment contains sharp edges. Ensure you are wearing PPE, have been fully    

                                      trained and use caution when using. 

5.5. Equipment Malfunction 

(i) 

If any part of the equipment fails or malfunctions, seek advice from the Laboratory Manager or 

Responsible Person. Unauthorized repairs may damage the

 microtome or alter its functionality, 

which may void the warranty. 

With permission of the Responsible Person, consult the 

operator’s instruction manual to access fault finding and troubleshooting procedures. 

 

(ii) 

If any problem cannot be rectified according to the troubleshooting procedures a “do not use” 

notice must be put on the microtome and the Laboratory Manager informed. The manufacturer 

must be contacted for advice and external maintenance or servicing might need to be 

arranged. 

 

(iii) 

All problems and corrective actions must be recorded in the equipment  maintenance log. 

             

 

(iv)  If the equipment fails to work or malfunctions and cannot be rectified according to troubleshooting 

procedures detailed in the Operator and Users Manuals the Laboratory Manager must be 

informed and a “Do Not Use” notice should be posted on the equipment. Contact the 

manufacturer for advice and coordinate with the Lab Manager for external maintenance and 

servicing.  

Summary of Contents for RM2125RTS

Page 1: ...laboratory staff shall i Work in accordance with the instructions for operation and maintenance detailed in this SOP Risk Assessment and the operator manual ii Carry out the routine inspection cleani...

Page 2: ...the system The Leica RM2125 RTS is a manually operated rotary microtome for creating thin sections of specimens of varying hardness for use in routine and research laboratories in the fields of biolo...

Page 3: ...lly or vertically as required 6 To clamp the cassette release the lever Note Before clamping the cassette into the universal cassette clamp remove excess wax on the outside of the cassette to ensure t...

Page 4: ...the Allen screw to release the clamp by turning Allen key No 4 9 counterclockwise 4 When using the knife holder base with lateral movement rotate the lever on the right side of the knife holder base c...

Page 5: ...ies to the rocking mode during which the specimen is trimmed by rocking motion no complete rotation of the handwheel Carry out rocking mode ONLY in the sectioning phase NEVER in the retraction phase 5...

Page 6: ...clockwise see curved arrow means the specimen advances Turning the coarse feed wheel clockwise means the specimen retracts away from the knife 2 Red dot at 12 o clock turning the coarse feed wheel clo...

Page 7: ...has 3 click stops 0 m 10 m and 50 m The points 23 mark the two trimming stages 10 m 50 m 1 To activate the trimming function press the lever downwards into the desired position and keep it pressed do...

Page 8: ...cimen clamp and mount a new specimen 4 Run the object clamp with the coarse feed back far enough until the new specimen can start being cut 5 4 Cleaning and Maintenance 5 4 1 Cleaning Note Always remo...

Page 9: ...ing the parts from the drying chamber 65 C Wearing safety gloves is recommended 8 After cleaning the moving parts apply a thin coat of drive part oil to them 9 Reassembly takes place in reverse order...

Page 10: ...e holder N The clamping lever 89 on knife holder E Shaft 90 of the clamping lever of the cassette clamp see Fig 36 Special Safety Note Equipment contains sharp edges Ensure you are wearing PPE have be...

Page 11: ...pproved by C Kavanagh Page 11 of 11 6 DOCUMENTATION 6 1 Equipment Maintenance Log 6 2 Microtome Training Agreement This form can be found on the Loughborough University LEARN module for the Centre of...

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