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Leica DM750

Manual

21

Substage Illumination

The Leica DM750 is available with two
types of Substage Illumination. Identify

which type of Illumination you have, as this will
be important to know later.

Type 1: Standard illumination

Adjustable condenser centering with provided
tool.

Type 2: Koehler illumination

Adjustable condenser centering with
thumbscrews and adjustable Koehler field
diaphragm.

Substage Illumination

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Summary of Contents for DM750

Page 1: ...Leica DM750 Manual...

Page 2: ...Industry Division Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland Responsible for contents Dietmar Gnass Product Compliance Vincent Vaccarelli Product Management In case of questions please...

Page 3: ...Leica DM750 Manual 3 Chapter overview Safety regulations 5 The Leica DM750 17 Ready 20 Set 30 Go 43 Care of the Microscope 45 Troubleshooting 48 Dimensions 51...

Page 4: ...n 18 Unpacking 19 Ready 20 Substage Illumination 21 Attaching the viewing Tubes 22 Leica EZ Viewing Tube integrated eyepieces 23 Leica Standard Viewing Tube separated eyepieces 25 Eyecups 26 Installin...

Page 5: ...Leica DM750 Manual 5 Safety regulations...

Page 6: ...rational safety main tenance and accessories The Safety concept booklet contains additi onal safety information regarding the service work requirements and the handling of microscope accessories and e...

Page 7: ...nt information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Danger due to hot surface This symbol...

Page 8: ...relevant user manuals Keep it in a safe place and readily accessible to the user User manu als and updates are also available for you to download and print from our website at www leica microsystems c...

Page 9: ...icroscope is not intended for use with biologically hazardous materials Caution The manufacturer assumes no liability for any noncompliant use or use outside of the specifications from Leica Microsyst...

Page 10: ...icals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electri cal devices outdoors Install electrical devices at least 10 cm from the wall and away fro...

Page 11: ...can be assembled and disassembled by the customer and pack them separately Disposal Once the product has reached the end of its service life please contact Leica Service or Sales about disposal Pleas...

Page 12: ...oscopy series are intended to reduce the exertion of the user to a minimum Danger of infection Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of th...

Page 13: ...ugg Switzerland immediately of any product defect that could potentially cause injury or harm If you use accessories made by third party manufacturers with the Leica DM750 microscope be sure that each...

Page 14: ...particles should be removed with a soft brush or lint free cotton cloth Remove coarse debris with a moistened disposable cloth Clinging dirt can be removed using petro leum ether or alcohol Acetone xy...

Page 15: ...the responsible service organization You can arrange for periodic inspections or if appropriate conclude a mainte nance contract with them It is advisable to enter a service agreement with Leica Servi...

Page 16: ...storage 20 90 Installation Category II Overvoltage Category Pollution degree 2 Fuse Replacement Unplug the instrument before changing any fuses The Leica DM750 has two fuses which are located behind t...

Page 17: ...Leica DM750 Manual 17 The Leica DM750...

Page 18: ...Microsys tems This model s exclusive design features and full range of accessories make it a truly versatile high quality instrument for educatio nal and routine clinical applications The instrument i...

Page 19: ...the planned configuration Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stan dard equipment These items are delivered in separat...

Page 20: ...Leica DM750 Manual 20 Ready...

Page 21: ...ation Identify which type of Illumination you have as this will be important to know later Type 1 Standard illumination Adjustable condenser centering with provided tool Type 2 Koehler illumination Ad...

Page 22: ...e 2 Standard viewing tube with separate eyepieces 1 Loosen the setscrew on top of the stand using the Allen key provided 2 Set the dovetail into the tripod mount and tighten the setscrew carefully wit...

Page 23: ...es To rotate the Leica EZ viewing tube either loosen the set screw on the stand or replace the set screw with the optional captive thumbscrew 1 In order to use the captive wingscrew remove the setscre...

Page 24: ...ca EZ viewing tube by loosening the captive thumbscrew rotating the viewing tube and tightening the captive thumbscrew again The eyepieces are integrated into the Leica EZ Viewing Tube and preset ther...

Page 25: ...a standard Phillips screwdriver not included in the delivery package The eyepieces will still rotate but they will be captive in the eyetubes The standard viewing tube has a rota table dovetail There...

Page 26: ...ht Eyecups If you have purchased a standard microscope configuration you will notice that the objectives are already installed on the nosepiece and the substage condenser is already installed on the s...

Page 27: ...rotating the objective nosepiece always use the knurled ring on the objective nosepiece As you rotate the nosepiece clockwise attach the objectives by screwing them into the nosepiece holes starting w...

Page 28: ...led 1 Move the specimen stage upwards as far as it will go by using the coarse focusing knob on the side of the microscope 2 Move the condenser holder into the lowest position by using the condenser f...

Page 29: ...using knob on the left side of the stage mount 6 Tighten the two setscrews using the tool included in the delivery package or for a Koehler stand tighten the two wingscrews so that the upper lens of t...

Page 30: ...Leica DM750 Manual 30 Set...

Page 31: ...owest setting to start with using the control ler on the bottom left of the stand The illumi nation control knob allows you to adjust the intensity of light produced by the illumination system Plug in...

Page 32: ...e highest position 2 Write an X on a piece of paper in the size of a business card and place it onto the light output of the microscope stand in such a way that the X is centered over the illumination...

Page 33: ...nd close this diaphragm simply turn the knurled ring on the condenser to the right or left so that the line on the ring is aligned with the objective magnification used 2 Open the iris diaphragm of th...

Page 34: ...he specimen stage by sliding it under the slide grips Slide grips hold the slide in place 2 Using the X Y stage control position the specimen slide such that a part of the speci men is under the objec...

Page 35: ...e coarse focusing knob as far as it will go to the maximum position 3 Look into the eyepieces and adjust the illu mination intensity to a level that is comfor table for your eyes The stand of the Leic...

Page 36: ...oehler field diaphragm continue with the Oil Immer sion Technique section on page 40 If you have a Leica DM750 with Koehler field diaphragm continue with the Koeh ler Configuration section on page 38...

Page 37: ...d ring on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus for this eye and this focusing eyepiece This corrects for any vision...

Page 38: ...r field diaphragm to the base of the microscope so that the diaphragm leaves are in the field of view when you look through the eyepieces 2 Focus the leaves of the field diaphragm using the condenser...

Page 39: ...9 3 Turn the condenser centering wingscrews simultaneously to center the image of the field diaphragm 4 Open the field diaphragm until the diaphragm leaves are just outside of the field of view Koehle...

Page 40: ...Move the specimen stage down to the lowest position using the coarse adjust ment knob 3 Add a drop of immersion oil from Leica to the area of the specimen slide you are studying 4 Rotate the oil imme...

Page 41: ...en bring the oil objective into the final position so that the oil drop is between the front lens of the objective and the specimen slide 7 Look through the microscope and slowly rotate the fine adjus...

Page 42: ...otate the intensity regulator to the lowest level 2 Switch on the instrument 3 Rotate the intensity regulator to the high est level and then back to the lowest level within five seconds The LED Illumi...

Page 43: ...Leica DM750 Manual 43 Go...

Page 44: ...44 Now all you need to do is change objectives set the condenser aperture and field diaphragm if you have a DM750 Koehler Stand appropriately for the objective magnification you are using and enjoy th...

Page 45: ...Leica DM750 Manual 45 Care of the Microscope...

Page 46: ...lastic dust cover provided with the instrument when it is not in use Instructions for cleaning If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syring...

Page 47: ...opropanol and squeeze it out until it is almost dry again Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface C...

Page 48: ...Leica DM750 Manual 48 Troubleshooting...

Page 49: ...the top Make sure that the cover slip thickness is correct and that it meets the specifica tions on the objective Dark field No definite DF contrast is possible Make sure that a DF objective is being...

Page 50: ...laced uniformly Check that the correct light ring is positioned Check the centering of the light rings Check the condenser centering Open the aperture diaphragm completely Specimen Stage Positioning r...

Page 51: ...Leica DM750 Manual 51 Dimensions...

Page 52: ...Leica DM750 Manual 52...

Page 53: ...Leica DM750 Manual 53...

Page 54: ...Leica DM750 Manual 54...

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