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Leica DM500 User Manual

Get Set! 29

Prepare to view a specimen slide

1. Place a specimen slide on the stage by slid-

ing the specimen slide forward into the
slide grips.

Slide grips hold the slide in place.

2. Use the X/Y stage control to position the

slide so a portion of the specimen is under
the objective in use.

Prepare to View a Specimen Slide

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Summary of Contents for DM500

Page 1: ...Leica DM500 User Manual ...

Page 2: ...2 Published in January 2015 by Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201 CH 9435 Heerbrugg Switzerland In case of questions please contact the hotline sms support leica microsystems com Manufacturer Information ...

Page 3: ...Leica DM500 User Manual 3 Chapter Overview Safety regulations 5 The Leica DM500 16 Get Ready 19 Get Set 26 Go 35 Care of the Microscope 37 Dimensions 40 ...

Page 4: ...and Ambient Conditions 15 The Leica DM500 Congratulations 17 Unpacking 18 Get Ready Attaching the Viewing Tubes 20 Leica EZ Viewing Tube Integrated Eyepieces 21 Leica Standard Viewing Tube Separated Eyepieces 23 Eyeguards 24 Installing Objectives and Substage Condenser 25 Get Set Turning on the Microscope 27 Using the Condenser 28 Prepare to View a Specimen Slide 29 Focusing 30 Viewing Tube Adjust...

Page 5: ...Leica DM500 User Manual Safety Regulations 5 Safety Regulations ...

Page 6: ... for their operational safety maintenance and accessories The Safety concept booklet contains addi tional safety information regarding the service work requirements and the handling of micro scope accessories and electrical accessories as well as general safety instructions You can combine individual system articles with articles from external suppliers Please read the user manual and the safety r...

Page 7: ...y impor tant information that is mandatory to read and observe Failure to comply can cause the following O O Hazards to personnel O O Functional disturbances or damaged instruments Danger due to hot surface This symbol warns against touching accessible hot surfaces e g those of light bulbs Important information This symbol indicates additional infor mation or explanations that intend to provide cl...

Page 8: ...readily accessible to the user User manu als and updates are also available for you to download and print from our website at www leica microsystems com Accessories from third party suppliers You can combine individual system articles with articles from external suppliers Please read the user manual and the safety requirements of the supplier Original condition To maintain the unit in its original...

Page 9: ...s Install electrical devices at least 10 cm from the wall and away from flam mable substances Avoid large temperature fluctuations direct sunlight and vibrations These conditions can distort measurements and micrographic images In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Non intended use Never install any other plug...

Page 10: ...e life please contact Leica Service or Sales about disposal Please observe and ensure compliance with the national laws and regulations that implement for example the EC Directive WEEE Like all electronic devices this instru ment its accessory components and consumables must never be disposed of with general household waste Disposal must comply with locally applicable laws and regula tions Integra...

Page 11: ... Leica microscopy series are intended to reduce the exertion of the user to a minimum Danger of infection Direct contact with eyepieces is a potential transmission method for viral infections of the eye The risk can be kept to a minimum by using personal eyepieces for each indi vidual or detachable eyecups Dangers during use O O The Leica DM500 microscope may only be connected to a grounded socket...

Page 12: ...435 Heerbrugg Switzerland immediately of any product defect that could potentially cause injury or harm O O If you use accessories made by third party manufacturerswiththeLeicaDM500micro scope be sure that each such manufacturer confirms the safety engineering harmless usability of the product and observe the product s user manual O O Modifications and maintenance of the Leica DM500 microscope may...

Page 13: ...rts and plastic parts O O Dust and dirt particles should be removed with a soft brush or lint free cotton cloth O O Remove coarse debris with a moistened disposable cloth O O Acetone xylene or nitro containing thin ners must NOT be used O O Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and specimens could be contaminated by abrade...

Page 14: ... werecommend that you take the precaution of contacting the responsible service organization You can arrange for periodic inspections or if appropriate conclude a mainte nance contract with them O O It is advisable to enter a service agreement with Leica Service O O For maintenance and repair only OEM spare parts may be used Repairs and service work O O Only original Leica Microsystems spare parts...

Page 15: ... pressure in use and for storage 500 1060 mBar Humidity in use and for storage 20 90 Installation Category II Overvoltage Category Pollution degree 2 Fuse replacement Unplug the instrument before changing any fuses The Leica DM500 has two fuses which are located behind the power cord receptacle Use only fuse type 5 20 mm 1 Amp 250 V Quick acting 13RFAG30003 Electrical data Leica DM500 Input 100 24...

Page 16: ...Leica DM500 User Manual The Leica DM500 16 The Leica DM500 ...

Page 17: ...ca DM500 17 Congratulations Thank you for purchasing the Leica DM500 Compound Microscope from Leica Micro systems This model s exclusive design features and full range of accessories make it a truly versatile high quality instrument ...

Page 18: ...t the planned configuration O O Optional items such as contrast accessories camera adapters cameras and carrying cases are not shipped as part of the stan dard equipment These items are delivered in separate packages O O Please do not discard any of the packing materials They should be used for safely storing and transporting the instrument should the need arise ...

Page 19: ...Leica DM500 User Manual Get Ready 19 Get Ready ...

Page 20: ...Type 2 Standard viewing tube with separate eyepieces 1 Slightly unscrew the set screw located on top of the stand with the Allen key tool provided 2 Insert the dovetail into the stand support and tighten the set screw firmly without forcing it This precisely positions the view ing tube onto the optical axis of the micro scope regardless of the selected rotation Attaching the Viewing Tubes ...

Page 21: ... Tube Integrated Eyepieces To rotate the Leica EZ viewing tube either loosen the set screw on the stand or replace the set screw with the optional captive thumbscrew 1 To use the captive thumbscrew completely remove the set screw that comes with the stand Be sure the thumbscrew portion of the captive thumbscrew is fully loosened before attaching it to the stand Leica EZ viewing tube with integrate...

Page 22: ... viewing tube by loosening the captive thumbscrew rotating the viewing tube and tightening the captive thumbscrew again The eyepieces are already integrated with the Leica EZ viewing tube and are pre adjusted for corrected vision therefore no eyepiece adjustment or insertion is necessary Go on to the section Eyeguards on page 24 ...

Page 23: ... eyetubes with a standard Cross type Phillips screwdriver not provided The eyepieces will still rotate but they will be captive in the eyetubes The standard viewing tube has a rotat able dovetail Therefore you can now rotate the standard viewing tube freely in any orientation 1 Insert the eyepieces into the eyetubes Standard viewing tube eyetubes do not yet contain the eyepieces ...

Page 24: ...lock out ambient room light Eyeguards If you have purchased a standard micro scope configuration you will notice that the objectives are already installed on the nose piece and the substage condenser is already installed on the stand In this case go to section Operation on page 27 If you purchased your Leica DM500 by components and not by stan dard configuration please continue to section Installi...

Page 25: ...clockwise attach the objectives by screwing them into the nosepiece holes starting with the lowest magnification and advancing to the highest magnification Substage condenser The Leica DM500 s substage condenser is pre installed pre focused and pre centered No additional installation or align ment is required Installing Objectives and Substage Condenser ...

Page 26: ...Leica DM500 User Manual Get Set 26 Get Set ...

Page 27: ...he power cord is not already attached attach it securely to the back of the microscop Setting the illumination intensity Set the illumination control knob located on the bottom left of the microscope stand to the lowest setting to start The illumination control knob allows you to adjust the intensity of light produced by the illumination system USB power connector The Leica DM500 has a 5 V 1 5 A U...

Page 28: ...tom right hand side of the stand Using the condenser The condenser is furnished with an iris diaphragm which can be adjusted to match the effective numerical aperture of each objective 1 To open and close the iris diaphragm simply rotate the knurled condenser ring to the right or to the left so that the line on the rotating ring aligns with the objective magnification in use 2 To start fully open ...

Page 29: ...lace a specimen slide on the stage by slid ing the specimen slide forward into the slide grips Slide grips hold the slide in place 2 Use the X Y stage control to position the slide so a portion of the specimen is under the objective in use Prepare to View a Specimen Slide ...

Page 30: ...nob to its positive stop at the highest position Coarse adjustment knob 3 Look into the eyepieces and adjust the illu mination intensity so that it is comfortable for viewing The Leica DM500 microscope stand has been factory adjusted so that the stage focus can be found within 1 5 rotations of the fine focus adjustment from this position 4 Using the fine adjustment knob bring the specimen into sha...

Page 31: ... has the eyepieces integrated with the eyetubes there are no additional adjust ments necessary Be sure you are wearing your eyeglasses or contact lenses Go on to section Oil Immersion on page 33 Standard viewing tube with two fixed eyepieces If you are using a standard viewing tube with two fixed eyepieces no focusing eyepieces there are no additional adjustments neces sary Be sure you are wearing...

Page 32: ...epiece with your other eye This time focus the specimen by using the focusing capability in the focus ing eyepiece Do not raise or lower the stage to focus 4 Grip the knurl on the focusing eyepiece with one hand and rotate the top of the eyepiece with the other hand until the specimen is in focus with this eye and this focusing eyepiece This corrects for any vision differences between your right e...

Page 33: ...ecimen that you were inspecting 4 Swing the oil immersion objective in place the objective will be labeled OIL 5 Raise the stage slowly using the coarse focus knob until the drop of oil on the spec imen slide just comes in contact with the front of the oil immersion objective lens 6 Grip the knurled nosepiece ring and rock the objective back and forth to eliminate any air bubbles then settle the o...

Page 34: ...raise the stage until the specimen is in focus 8 When finished using the oil immersion objective be sure to clean the front of the objective the specimen slide and any other surfaces that the oil contacted using the instructions in section Care of the Microscope on page 37 Oil Immersion Technique Continued ...

Page 35: ...Leica DM500 User Manual Go 35 Go ...

Page 36: ...Leica DM500 User Manual Go 36 Now all you need to do is change objectives set the condenser appropriately for the objective magnification in use and enjoy the view Ready Set Go ...

Page 37: ...Leica DM500 User Manual Care of the Microscope 37 Care of the Microscope ...

Page 38: ...plastic dust cover provided with the instrument when it is not in use O O If any optical surface becomes coated with dust or dirt clean the surface by blowing it off with a syringe or brushing it off with a camel hair brush before attempting to wipe the surface clean O O Optical surfaces should be cleaned with a lint free cloth lens tissue or cotton swab moistened with a commercially available gla...

Page 39: ...e of this front lens can be readily cleaned with a toothpick covered with a cotton tip or with a small cotton swab Moisten the cotton with commer cially available glass cleaner Wipe the front lens lightly without applying undue force or scrubbing action Make sure that the cotton tip contacts the concave lens surface Check the objective with a magni fier after cleaning O O If you need to remove the...

Page 40: ...Leica DM500 User Manual Dimensions 40 Dimensions ...

Page 41: ...Leica DM500 User Manual Dimensions 41 ...

Page 42: ...Leica DM500 User Manual Dimensions 42 ...

Page 43: ...Leica DM500 User Manual Dimensions 43 ...

Page 44: ...Leica DM500 User Manual Dimensions 44 ...

Page 45: ...Leica DM500 User Manual Dimensions 45 ...

Page 46: ...ation and educational applications Leica Microsystems an international company with a strong network of worldwide customer services Active worldwide Tel Fax Australia North Ryde 61 2 8870 3500 2 9878 1055 Austria Vienna 43 1 486 80 50 0 1 486 80 50 30 Belgium Diegem 32 2 790 98 50 2 790 98 68 Brazil São Paulo 55 11 2764 2411 11 2764 2400 Canada Concord Ontario 1 800 248 0123 847 405 0164 Denmark B...

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