Instructions for Use
English
Order No.: 14 0491 80101 - Revision N
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Version 2.0, Revision N - 10.2019
Leica CM1860/CM1860 UV
Cryostat
Page 1: ...ns for Use English Order No 14 0491 80101 Revision N Always keep this manual with the instrument Read carefully before working with the instrument Version 2 0 Revision N 10 2019 Leica CM1860 CM1860 UV...
Page 2: ......
Page 3: ...wings illustrations and other information regarding the contents or technical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are d...
Page 4: ...1860 CM1860 UV cryostat 23 4 3 Standard delivery packing list 26 4 4 Installing the handwheel 27 5 Instrument Setup 28 5 1 Electrical connection 28 5 2 Preparations for starting up the instrument 28 5...
Page 5: ...lection chart in minus C 61 7 6 Defrosting 62 7 6 1 Automatic defrosting of the cryochamber 62 7 6 2 Manual defrosting of the cryochamber 62 7 6 3 Manual defrosting of the quick freeze shelf 63 7 7 En...
Page 6: ...s 79 10 1 Ordering information 79 10 2 Mobile heat extractor application 102 10 3 Cold extraction using the Thermal block 103 10 4 Stationary heat extractor optional application 104 10 5 Installing th...
Page 7: ...V C Description Caution UVC radiation Symbol Title of the symbol Item number Fig 7 1 Description Item numbers for numbering illustrations Numbers in red refer to item numbers in illustrations Symbol T...
Page 8: ...of the maximum permitted limit Symbol Title of the symbol CSA test symbol USA Canada Description The CSA test symbol means that a product has been tested and fulfills the applicable safety and or per...
Page 9: ...rohibited due to explosion hazard Symbol Title of the symbol IPPC symbol Description The IPPC symbol includes IPPC symbol Country code in accordance with ISO 3166 e g DE for Germany Regional identifie...
Page 10: ...the shipment has been transported and stored in upright position according to your requirements With a pitch of 60 or more the blue quartz sand flows into the arrow shaped indicator window and sticks...
Page 11: ...pe All information contained in these Instructions for Use applies exclusively to the instrument type indicated on the title page A nameplate bearing the instrument serial number SN is attached to the...
Page 12: ...r other personnel at risk of injury or can cause damage to the instrument or other property The machine may be used only as intended and only if all of its safety features are in proper working condit...
Page 13: ...n can never be used as a substitute for manual protection in accordance with laboratory regulations UVC disinfection is effective when disinfecting surfaces and air within the irradiated working area...
Page 14: ...Warning Always lock the handwheel prior to manipulating the knife and specimen changing the specimen or taking a break To lock the handwheel move its handle to the 12 o clock Fig 1 1 or 6 o clock Fig...
Page 15: ...of the instrument it must be set up while maintaining a minimum distance on all sides from walls and furniture rear 15 cm right 30 cm left 15 cm Electrical connection Before connecting the instrument...
Page 16: ...fe blade is covered by the safety guard Extended skin contact with the cold parts of the instrument may cause freezer burns To make sure that the condensation water stemming from the defrost cycles dr...
Page 17: ...gulations on disposal of special category waste Refer to P 71 9 2 Disinfection at room temperature for information on disinfection at room temperature After each disinfection the chamber must be wiped...
Page 18: ...ng the lamp For further information refer to P 71 9 Cleaning Disinfection Maintenance Warning It is possible to break the UVC lamp during replacement If this happens the lamp change must be completed...
Page 19: ...C 240 VAC Nominal frequency 50 60 Hz 60 Hz 50 60 Hz 50 60 Hz Power consumption 1440 VA 1440 VA 1440 VA 1440 VA Power supply IEC 60320 1 C 20 Max start up current for 5 sec 45 A eff 45 A eff 45 A eff 4...
Page 20: ...29 IP20 Heat emission 1440 J s A weighted noise level measured at 1 m distance 70 dB A EMC class B FCC Rules part 15 B CISPR 11 IEC 61326 CAN ICES 3 A NMB Warning Observe P 22 4 1 Installation site re...
Page 21: ...of freezing stations 2 Duration of active time 10 minutes turn off possible after 6 minutes Defrosting Together with quick freeze shelf Warning Refrigerant and compressor oil must be replaced by serv...
Page 22: ...ndoor use only The power plug circuit breaker must be freely and easily accessible The power socket may not be located further than 2 5 m from the instrument Power supply at a distance no greater than...
Page 23: ...g 4 1 suitable shears and safety gloves are required Stand next to the packaging and cut through the strips at the locations shown Fig 4 3 Lift up the covering box Fig 4 2 and remove it Carefully remo...
Page 24: ...24 Version 2 0 Revision N Installing the Instrument 4 Fig 4 1 2 3 Fig 5 6 1 2 2 5 4 3 Fig 6 1 Fig 7 1 1 7 2 6 5 4 3...
Page 25: ...to the desired location and observe the following notes Warning The instrument must be transported in either an upright position or slightly tilted max 30 When tilting the instrument 2 people must cou...
Page 26: ...137 1 Allen key size 3 0 14 0222 04138 1 Allen key size 4 0 14 0222 04139 1 Allen key with ball head size 4 0 14 0222 32131 1 Allen key size 5 0 14 0222 04140 1 Key with handle size 5 0 14 0194 04760...
Page 27: ...ig 9 1 2 3 4 To install the handwheel follow these steps 1 Insert the pin Fig 9 1 of the handwheel shaft into the hole Fig 9 2 of the handwheel 2 Attach the spring washer Fig 9 3 to the screw as shown...
Page 28: ...he values on the nameplate of the instrument Warning Use only the power cord provided by Leica Failure to observe this causes an imminently hazardous situation that may result in death or serious inju...
Page 29: ...nstrument Setup 5 Warning We do not recommend placing disposable blade dispensers in the cryochamber for pre cooling because it is possible that individual blades may be stuck together during removal...
Page 30: ...30 Version 2 0 Revision N Instrument Setup 5 5 3 General overview of the Leica CM1860 CM1860 UV 17 21 13 16 13 14 13 12 11 10 9 8 23 18 20 19 15 Fig 10...
Page 31: ...5 Storage shelf left 17 Orientable specimen head 6 Storage shelf right 18 Blade knife holder base optional 7 Automatic fuse and ON OFF switch 19 Blade holder CE optional 8 Quick freeze shelf 20 Knife...
Page 32: ...this may cause damage to the instrument The software version 0 40 shown here Fig 13 only as an example is displayed in the 4 digit LED display for the time This display disappears after approx 2 secon...
Page 33: ...d parameters from unintended modifications Enable disable the lock by pressing and holding for 5 seconds UVC disinfection CM1860 UV only Note Only CM1860 UV Before UVC disinfection move the anti roll...
Page 34: ...tion Press the UVC button briefly 1 second The LED for the short disinfection cycle will light up Start long disinfection Press the UVC button for longer 1 second The LED for the short disinfection go...
Page 35: ...e of the UVC lamp has expired both LEDs flash alternately Note The disinfection mode is aborted as soon as the sliding window of the cryostat is open or the operator presses the UVC button In the case...
Page 36: ...nts touch or press and hold the Plus or Minus button The defrost duration is 12 minutes 6 2 3 Programming the temperature of the cryochamber Fig 17 1 2 The temperature of the cryochamber is set and in...
Page 37: ...y pressing the key Fig 18 2 If the Peltier element has been activated a blue LED in the Peltier symbol lights up Fig 18 3 After the activation the display switches to the number 10 Fig 18 4 the Peltie...
Page 38: ...g the key Fig 19 1 continuous tone sounds followed immediately by the key Fig 19 2 continuous tone stops The display flashes during the defrosting To shut off the manual defrost early press the Fig 19...
Page 39: ...emperature selection field 6 2 7 Setting the section thickness Fig 21 1 2 3 Adjust the section thickness in a range from 1 100 m using the Plus Fig 21 1 and Minus Fig 21 2 pushbuttons in the upper con...
Page 40: ...instrument in the initialization phase press the Plus button Fig 21 2 If the handwheel is at the 6 o clock position or the specimen head is down the yellow LED next to Retract Fig 21 3 is not illumina...
Page 41: ...reached Fig 23 1 The return movement can be stopped by pressing one of the coarse feed buttons Slow Slow return to the rear end position starts The motion continues as long as the button is held Adva...
Page 42: ...tment 7 Check whether retraction is as wanted on or off P 40 6 3 Retraction Sectioning 1 Select trimming thickness 2 Adjust anti roll device P 51 Adjusting the anti roll guide system 3 Trim using the...
Page 43: ...ecessary enable the Peltier element Fig 24 4 it takes approx 60 seconds until the maximum cooling capacity is available 3 Apply a sufficient amount of cryocompound to a specimen disc that is at room t...
Page 44: ...lamping screw Fig 25 1 at the specimen head 4 Insert the shaft of the specimen disc Fig 25 3 with the frozen specimen in the location hole Fig 25 2 of the specimen head Note Make sure that the shaft o...
Page 45: ...4 4 Adjust the clamping force of the t piece To ensure a proper sectioning result the knife holder base Fig 26 1 must be securely clamped onto the base plate Fig 27 1 The clamping is carried out using...
Page 46: ...ile being raised If necessary move the specimen backwards to avoid collisions during further trimming A clearance angle that is too steep or too shallow may lead to suboptimal sectioning results and c...
Page 47: ...Note Please observe the Instructions for Use included in the packaging for handling the premium blade holder 7 4 4 Blade holder CE Note If additional blade holder Instructions for Use have been provi...
Page 48: ...ust be worn when inserting the blade 1 Fold the anti roll guide system Fig 31 4 towards the left while doing so hold the lever Fig 31 11 NOT the adjusting screw of the anti roll guide so that the heig...
Page 49: ...rest Fig 33 must first be placed in the blade holder followed by the blade Fig 33 Two magnets Fig 34 are attached to the rear side of the guide These point away from the operator after the guide has b...
Page 50: ...brush with magnet Fig 36 12 To do so fold the clamping lever Fig 36 10 downward counterclockwise Fig 36 Fold the anti roll guide system Fig 36 4 to the left Guide the brush with magnet Fig 36 1 to the...
Page 51: ...esired position 2 To tighten turn the clamping lever Fig 38 14 to the front clockwise Fig 38 14 Adjusting the anti roll guide system You can adjust the height of the anti roll guide system using the k...
Page 52: ...thicknesses e g 10 m Start from there and work your way down to the desired section thickness in small increments readjusting the anti roll guide system at each increment using the knurled nut Assembl...
Page 53: ...spacers 70 mm 50 m for section thickness 4 m 70 mm 100 m for section thickness 5 m 50 m 70 mm 150 m for section thickness 50 m Note All 4 longitudinal edges of the anti roll glass plate can be used 7...
Page 54: ...Note When inserting the blade knife holder base overcome the resistance of a spring located in the foot of the knife holder by applying a little pressure towards the left 2 To clamp the blade knife h...
Page 55: ...ide above the knurled screw Fig 45 4 so that the notch Fig 45 24 faces the user turn the knurled screws for height adjustment until the lower position is reached The knife can now be inserted from the...
Page 56: ...ety guard Fig 47 18 is secured and integrated into the clamping jaws The safety guard has handles Fig 47 19 that allow it to be moved The safety guard is suitable for knives up to 16 cm in length Alwa...
Page 57: ...ig 47 Knife holder CN with anti roll guide Moving the clamping jaws Note If large specimen discs are to be used with the knife holder e g 50 x 80 mm it is possible to shift the clamping jaws laterally...
Page 58: ...Now use the longer knife backrest provided 5 6 Fig 50 7 4 7 Cleaning the blade and knife holders Note For daily cleaning just remove the section waste from the blade knife holder using a dry brush Pl...
Page 59: ...er Fig 51 11 3 Loosen the pressure plate clamping lever Fig 51 10 by turning it counterclockwise and pull it out 4 Afterwards the pressure plate Fig 51 1 can be removed for cleaning with alcohol 5 Loo...
Page 60: ...lade or specimen prior to changing the specimen block and during all work breaks 1 Insert the pre cooled knife disposable blade into the knife blade holder 2 Adjust the knife holder to an appropriate...
Page 61: ...Cartilage Cervical Fatty Heart and vascular Intestinal Kidney Laryngeal Lip Liver Lung Lymphoid Muscle Nose Pancreatic Prostate Ovarian Rectal Skin with fat Skin without fat Spleen or bloody tissue Te...
Page 62: ...mber defrosting However the Peltier element is turned off The maximum duration of a defrost cycle is 12 minutes Defrosting is terminated automatically once the cryochamber has reached a temperature of...
Page 63: ...it back into the knife case blade dispenser in the cryochamber 3 Remove frozen section waste with a cold brush 4 Empty the section waste tray 5 Clean the storage shelves and brush shelf Note Only comm...
Page 64: ...ser Fig 37 3 Remove all specimen material from the cryostat 4 Remove the blade knife holder out of the cryochamber 5 Remove section waste with a cold brush 6 Empty the section waste tray 7 Switch the...
Page 65: ...terval warning after 15960 hours Call service 30 Service interval expired after 17610 hours Call service Note If errors occur they are output as error codes in the real time display during normal oper...
Page 66: ...dings is constantly higher than 40 C Drop the temperature of the immediate surroundings 2 When setting up the instrument the minimum clearance P 22 4 1 Installation site requirements to walls and furn...
Page 67: ...ber temperature Sections splinter cracks in the section Specimen too cold Select higher temperature Sections not properly flattened Static electricity air currents Eliminate cause Specimen not cold en...
Page 68: ...disc Refreeze specimen onto the disc Very hard inhomogeneous specimen Increase section thickness reduce specimen surface area if necessary Dull knife Use a different part of the knife Knife profile i...
Page 69: ...rect clearance angle Dried specimen Prepare new specimen Tissue adheres to the anti roll plate Anti roll plate is too warm or incorrectly positioned Cool down anti roll plate or reposition correctly S...
Page 70: ...e microtome Friction between the slot cover and the microtome housing Apply cryo oil to the slot cover and distribute it by moving the handwheel or using a clean cloth Both disinfection indicator ligh...
Page 71: ...rument is switched off Before switching on the instrument again proper ventilation of the chamber shall be ensured Note If the cryostat is completely defrosted the rubber plug on the floor of the cryo...
Page 72: ...e The waste container Fig 55 1 collects the condensate that accumulates during defrosting Therefore check the level regularly and empty the container according to applicable laboratory regulations 9 3...
Page 73: ...wards by turning the handwheel apply a few drops of cryo oil to the slot cover 2 Then move the specimen head all the way down and apply a few drops of cryo oil to the slot cover 3 Distribute the oil b...
Page 74: ...2 Replacing the fuses Warning Turn the instrument off and disconnect the power plug before replacing the fuses Use only the fuse types specified in P 19 3 Technical Data Failure to comply with this c...
Page 75: ...ty F2 Coarse feed T 1 6 A F3 Processor board power supply T 1 0 A F4 Heaters T 6 25 A F5 Peltier element T 4 A 9 3 3 Replacement of the UVC lamp Warning Switch the instrument off and disconnect the po...
Page 76: ...Fig 60 1 holding it by the grip Fig 60 2 and pull it out to the front 1 2 Fig 60 Note For the CM1860UV two different variants of the connection piece inside the instrument and the UVC Lamp are availab...
Page 77: ...th 4 pin inlet Fig 62 2 UVC lamp 4 pin Fig 62 1 1 2 Fig 62 Removal of the lamp The UVC lamp Fig 63 1 is installed in front of the protection screen for chamber illumination Fig 63 2 1 Hold the lamp wi...
Page 78: ...the left side until it engages 3 Screw the metal ring onto the holder then hold the lamp with both hands and carefully push it into the clips Fig 63 3 4 Reinsert the sliding window 5 Connect the inst...
Page 79: ...14 0216 26379 Leica high profile disposable blades Type 818 1 pack of 50 14 0358 38926 Leica high profile disposable blades Type 818 10 packs of 50 14 0358 38383 Leica low profile disposable blades T...
Page 80: ...43247 Red O ring for color marking 20 mm and 30 mm 10 pcs 14 0477 43248 Blue O ring for color marking 40 mm 10 pcs 14 0477 43249 Red O ring for color marking 40 mm 10 pcs 14 0477 43250 Blue O ring fo...
Page 81: ...50 mm 14 0419 33816 FSC22 tissue freezing medium 9 x 118 ml clear1 380 1480 FSC22 tissue freezing medium 9x118 ml blue1 380 1481 Tissue freezing medium 125 ml 14 0201 08926 Cryo oil 250 ml 14 0336 061...
Page 82: ...nd short knives Anti roll guide and adjustable safety guard Order No 14 0477 42358 Fig 69 Blade holder CE universal high profile and low profile disposable blades with lateral movement and glass anti...
Page 83: ...artilage Order No 14 0491 47874 Fig 72 Blade holder pressure plate 22 for high profile microtome blades Order No 14 0491 48004 Fig 73 Anti static kit for blade holder CE high profile Order No 14 0800...
Page 84: ...sectioned The one of a kind fine grain carbide metal guarantees sections to approx 2 m The blades are fully recyclable Length 65 mm thickness 1 mm Height 11 mm Leica TC 65 tungsten carbide disposable...
Page 85: ...8 Knife 16 cm steel profile c flat on both sides for wax and frozen sections Serial number of the knife Fig 78 1 Note including knife case 14 0213 11140 Order No 14 0216 07100 Fig 79 Knife 16 cm tungs...
Page 86: ...rofile d Note including knife case 14 0213 11140 Order No 14 0216 07132 Knife 16 cm tungsten carbide profile d Note including knife case 14 0213 11140 Serial number of the knife Fig 80 1 Order No 14 0...
Page 87: ...tages 16 dispensing slides 1 sectioning board freezing stage 1 heat extractor elevated 1 embedding forceps angled Order No 14 0201 40670 Fig 83 Cryoembedding system assembly consisting of 3 embedding...
Page 88: ...ith medium sized recesses consisting of 1 embedding well bar 24 mm recesses 4 specimen stages small 2 heat extractors 8 dispensing slides Order No 14 0201 39117 Embedding well bar set with large reces...
Page 89: ...ight 25 4 mm with 4 recesses size 24 mm stainless steel Order No 14 0201 39121 Height 25 4 mm with 3 recesses size 30 mm stainless steel Order No 14 0201 39122 Fig 87 Specimen stage rectangular stainl...
Page 90: ...Revision N Ordering Information Components and Consumables 10 Fig 89 Container for specimen stages Order No 14 0201 39126 Fig 90 Dispensing slides for Dr Peters cryoembedding system Pack of 8 Order No...
Page 91: ...ng for color marking the 55 mm specimen discs pack of 10 Order No 14 0477 43251 Red O ring for color marking the 55 mm specimen discs pack of 10 Order No 14 0477 43252 Fig 92 Toolset for cryostats con...
Page 92: ...r 12 specimen slides gray Pack of 6 Order No 14 0712 40161 Fig 94 Easy Dip staining container Color white Pack of 6 Order No 14 0712 40150 Color pink Pack of 6 Order No 14 0712 40151 Color green Pack...
Page 93: ...ormation Components and Consumables 10 Fig 95 Specimen disc 20 mm Order No 14 0370 08636 25 mm Order No 14 0416 19275 30 mm Order No 14 0370 08587 40 mm Order No 14 0370 08637 55 mm Order No 14 0419 2...
Page 94: ...4 5 9 Fig 96 1 Heat extractor stationary optional 6 Brush shelf 2 Peltier element with 2 startions 7 Specimen head orientable 3 Freeze shelf 8 positions 8 Waste tray 4 Shelf movable optional 9 Storag...
Page 95: ...onsumables 10 Fig 97 Transfer block for specimen discs large 13 slots Order No 14 0491 47786 Fig 98 Transfer block for specimen discs small 5 slots Order No 14 0491 47787 Fig 99 Large transfer blocks...
Page 96: ...obile P 102 10 2 Mobile heat extractor application Order No 14 0443 26836 Fig 101 Thermal block for easy removal of frozen tissue from the specimen disc P 103 10 3 Cold extraction using the Thermal bl...
Page 97: ...2 Order No 14 0419 35693 Fig 104 Anti roll guide system CE Glass 70 mm consisting of Glass insert 70 mm Metal frame for exchangeable glass inserts CE 100 m spacer Recommended section thickness 5 m 50...
Page 98: ...50 m For blade holder CE 14 0491 47873 14 0419 33992 Order No 14 0419 37260 Fig 107 Anti roll guide system for knife holder CN glass 50 mm consisting of Glass insert 50 mm Metal frame for exchangeable...
Page 99: ...mbedding medium FSC 22 for cryosectioning FSC 22 is available in clear or in light blue for better visualization of small specimens Clear 9 x 118 ml Order No 380 1480 Blue 9 x 118 ml Order No 380 1481...
Page 100: ...Information Components and Consumables 10 Fig 112 Cryo oil 250 ml bottle Order No 14 0336 06100 Fig 113 Cut resistant safety gloves 1 pair size S Order No 14 0340 40859 Cut resistant safety gloves 1...
Page 101: ...Components and Consumables 10 1 Fig 114 Heat extractor stationary assembly Order No 14 0471 30792 Low temperature buffer for heat extractor parking station Fig 114 1 Order No 14 0471 30793 Fig 115 UV...
Page 102: ...46750 10 2 Mobile heat extractor application Specimen freezing with the freezing shelf can be accelerated by the additional use of a heat extractor 1 Store the heat extractor in the cryochamber 2 Hol...
Page 103: ...stored outside at room temperature 1 Place the cap Fig 118 5 on the required side so that the appropriate location hole for the specimen disc is visible 2 Fit the pin Fig 118 2 of the specimen disc F...
Page 104: ...l down Move the heat extractor from the parking station and lower it directly onto the surface of the specimen until it is completly frozen After the specimen is frozen move the heat extractor into re...
Page 105: ...chased the instrument Please provide the following information about the instrument Model name and serial number of the instrument Location of the instrument and name of a contact person Reason for th...
Page 106: ...he product menu This template must be used to record all required data If a product is returned a copy of the completed and signed decontamination certificate must either be enclosed or handed over to...
Page 107: ......
Page 108: ...ww LeicaBiosystems com Version 2 0 Revision N 10 2019 Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems com...
