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Leica BIOSYSTEMS 1404723512, Instructions For Use Manual, Page: 12 / 42

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Leica BIOSYSTEMS 1404723512 Instructions For Use Manual Download Page 12

12

Version 2.4, Revision L 

Instrument Characteristics 

3

3.2 

General overview - VT1000 S

Fig. 1 

 

7

8

1

2

3

4

5

6

9

10

 

1

Fixture for magnifier support

6

Buffer tray S

2

Control panel

7

Magnifier support

3

EMERGENCY STOP switch (not visible here)

8

Magnifier

4

Cutting head

9

Module Hi-Power spot, LED 1000 (optional)

5

Knife holder

10

Module LED Hi-Power spots, 2-arm 

(optional)

2

3 4

Fig. 2 

 

1

5

 

1

EMERGENCY STOP button

3

Connecting socket for power cord

2

Fixing device for drain tube

4

Power switch

5

Connection for foot switch, 9-pole

Summary of Contents for 1404723512

Page 1: ...ibrating blade microtome Instructions for Use English Order No 14 0472 80101 Revision L Always keep this manual with the instrument Read carefully before working with the instrument Version 2 4 Revision L 11 2019 ...

Page 2: ......

Page 3: ...ation regarding the contents or technical details of the present Instructions for Use are not to be considered warranted characteristics of our products These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing processes without prior notice Only in this way is it possible to ...

Page 4: ...ation site requirements 18 5 2 Setting up the instrument 18 5 3 The operating elements and their function VT1000 S 20 5 4 Adjusting the amplitude 24 5 5 Working with the VT1000 S on a daily basis 24 5 6 Routine daily maintenance and switching off the instrument VT1000 S 28 6 Malfunctions Meaning and Troubleshooting 29 7 Cleaning and Maintenance 33 7 1 Cleaning the instrument 33 7 2 Changing the fu...

Page 5: ... property damage which if not avoided could result in damage to the machine or something in its vicinity Symbol Title of the symbol Item number Fig 7 1 Description Item numbers for numbering illustrations Numbers in red refer to item numbers in illustrations Symbol Title of the symbol Function key Start Description Software symbols that have to be pressed on the input screen are displayed as bold ...

Page 6: ...of the symbol Order No Description Indicates the manufacturer s catalog number so that the medical device can be identified Symbol Title of the symbol Serial number Description Indicates the manufacturer s serial number so that a specific medical device can be identified Symbol Title of the symbol Fragile handle with care Description Indicates a medical device that can be broken or damaged if not ...

Page 7: ... the symbol Tilt indicator Description Tip n Tell indicator to monitor whether the shipment has been transported and stored in upright position according to your requirements With a pitch of 60 or more the blue quartz sand flows into the arrow shaped indicator window and sticks there permanently Improper handling of the shipment is immediately detectable and can be proven definitively Symbol Title...

Page 8: ...4 Qualification of personnel The VT1000 S should be operated by trained laboratory personnel only The instrument is intended for professional use only All laboratory personnel designated to operate this instrument must read these Instructions for Use carefully and must be familiar with all technical features of the instrument before attempting to operate it 1 5 Intended use improper use The VT1000...

Page 9: ...ve all notes and warnings contained in these Instructions for Use The current EC Declarations of Conformity can be found on the Internet www LeicaBiosystems com Note These Instructions for Use must be appropriately supplemented as required by the existing regulations on accident prevention and environmental safety in the operator s country Warning The protective devices located on the instrument a...

Page 10: ...ades Do not leave knives blades and bladed knife holders unprotected All appropriate safety precautions must be met to avoid the risk of infection Protective clothing according to safety regulations for Working with harmful substances Safety mask gloves protective clothing must be worn Cover the magnifier during work breaks as it may act as a burning glass when not covered In case of emergency pre...

Page 11: ...tion 330 Section thickness selection 1 999 μm in 1 μm steps Magnifier assy standard accessory of the configured instrument 2 x magnification Ambient conditions Operating temperature range min 5 C to max 40 C Relative humidity max 80 Height up to 2000 m above sea level Electrical data Rated voltage range 10 100 V 240 V Nominal frequency 10 50 60 Hz Power draw 50 VA Mains fuse T 1 25 A Pollution deg...

Page 12: ... 2 Control panel 7 Magnifier support 3 EMERGENCY STOP switch not visible here 8 Magnifier 4 Cutting head 9 Module Hi Power spot LED 1000 optional 5 Knife holder 10 Module LED Hi Power spots 2 arm optional 2 3 4 Fig 2 1 5 1 EMERGENCY STOP button 3 Connecting socket for power cord 2 Fixing device for drain tube 4 Power switch 5 Connection for foot switch 9 pole ...

Page 13: ...ED mode indication Section thickness 4 selection button for section thickness 1 999 μm selectable retraction and or volume 11 CLR Clear button 5 Button for setting the limit stops of the sectioning window 12 DISP Programming button 6 Start button for single continuous sectioning stroke 13 Selector button Single continuous stroke LEDs indicate selected mode 7 Pause button stops sectioning process 1...

Page 14: ...cimen discs S non orientable 14 0463 27404 Buffer tray S 14 0462 30132 5 Countersunk screw M 5 x 8 14 2101 77121 2 Hose clamps 14 0481 41952 Knife holder S for injector and razor blades 14 0462 30131 Hexagon key w handle size 3 14 0194 04764 1 Bottle of Cyanoacrylate adhesive 14 0371 27414 Magnifier assy magnifier glass carrier 14 0462 31191 The country specific power cord needs to be ordered sepa...

Page 15: ...scribed In this case please mark the shipping documents accordingly and check the shipment for damage 1 2 3 4 5 6 Fig 4 Remove the packing strap and the adhesive tape Fig 4 1 Remove the carton lid Fig 4 2 Take out the accessory carton Fig 4 3 Take Allen key No 8 out of the accessory carton and put it aside for later use Remove the fixing carton Fig 4 4 Remove the outer carton wall Fig 4 5 Lift the...

Page 16: ...g the size 8 Allen key provided and remove the base plate Fig 6 Using both hands at the sides Fig 6 grasp the bottom of the instrument and carefully place it on a suitable laboratory table Note Compare with the attached packing list to make sure the delivery is complete Note The transport carton and included retaining elements should be kept in case a return shipment is necessary later To return t...

Page 17: ...er Secure the loose end of the drain tube in the holder at the rear of the instrument Fig 7 2 Fig 7 1 2 Assembling the magnifier support and foot switch optional The magnifier support Fig 8 1 is packaged separately Set it on the instrument as shown in Fig 8 Attach the optional foot switch Securely plug the foot switch into the 9 pin socket Fig 8 2 Fig 8 2 1 Note When transporting the instrument al...

Page 18: ...e fluctuations Warning The instrument may not be operated in hazardous locations Warning The instrument MUST be connected to a grounded power socket Use only a provided power cable that is intended for the local power supply 5 2 Setting up the instrument Note The instrument MUST be connected to a grounded power socket Use only a provided power cable that is intended for the local power supply Warn...

Page 19: ...Insert the knife holder 6 Insert a blade into the knife holder 7 Connect the magnifier support with Module LED Hi Power spots 2 arm as shown in Fig 9 Insert plug Fig 9 1 of the Module LED Hi Power spots 2 arm into socket Fig 9 2 at the Module Hi Power spot LED 1000 Fig 9 1 2 8 Connect the optional foot switch at the rear of the instrument 9 Plug the power cable into the wall socket 10 Switch the i...

Page 20: ...5 1 0 050 2 0 075 3 0 125 4 0 175 5 0 225 6 0 40 7 0 65 8 0 90 9 1 30 10 2 50 Continuous knife feed adjustment from 0 05 2 5 mm s Knife return stroke is performed at constant speed of 5 mm s The additional locking lever Fig 10 1 lever in 12 o clock position prevents the speed setting from being accidentally changed while sectioning is in progress Fig 11 FREQ Rotary knob with scale from 0 to 10 Sca...

Page 21: ...en block and press once more to set the second sectioning window limit Press START to deactivate V Max The knife edge moves back to the first sectioning window limit and resumes sectioning at the previously selected speed 10 speed rotary potentiometer Function Start single or continuous sectioning stroke according to whether SINGLE or CONT mode has previously been selected p 22 LED indication with...

Page 22: ... a sectioning stroke which has been activated by pressing the START STOP button Fig 13 LED indication with adjusting button DISP and CLR function keys Function of LED indication Indicates the selected sectioning thickness or totalized section thickness Function of the button Selection of section thickness in 1 μm steps from 0 to 999 μm The specimen feed in the preselected section thickness takes p...

Page 23: ...ile the knife is in motion the UP DOWN toggle switch is inoperational For the DOWN motion the toggle switch can be locked in the DOWN position For the UP motion the switch must be pressed and held in the UP position When the lowest possible position is reached with the toggle switch being locked in DOWN there will be both an audible and a visible signal Once the switch is unlocked the buffer tray ...

Page 24: ... from left to right 0 2 mm 0 4 mm 0 6 mm 0 8 mm 1 mm Slide the amplitude clamping screw to the desired amplitude position and retighten Note When adjusting the amplitude setting do not remove the clamping screw simply loosen it The instrument is shipped with the amplitude set to 0 6 mm 5 5 Working with the VT1000 S on a daily basis Fig 18 1 2 4 3 Mount the buffer tray Fig 18 1 onto the bolt Fig 18...

Page 25: ...e buffer tray using the manipulator Fig 20 2 Fig 21 2 1 Use the manipulator Fig 21 2 to rotate the specimen disc into the desired position Tighten with a No 3 Allen key Fig 21 1 The clamping screw or one of the clamping devices must not be located over the gap in the specimen disc as in these positions clamping the specimen disc is not possible Remove the manipulator Fig 21 2 ...

Page 26: ...elected clearance angle by tightening the two screws Fig 22 2 Note The VT1000 S does not require the readjustment of the clearance angle every time you change the blade Make an adjustment only if required by an application for technical reasons e g different type of tissue Fig 23 1 To insert the blade loosen the clamping screw Fig 23 1 located on the knife holder Clean the blade Fig 24 1 2 Insert ...

Page 27: ...o select a sectioning thickness for trimming Select a sectioning range appropriate to the size of the specimen with the SECTIONING WINDOW button Switch the SINGLE CONT button to CONT Push the START STOP button The instrument will now trim the specimen at the selected trimming thickness until you push the START STOP button once more Once you have reached the desired specimen plane for sectioning us...

Page 28: ...ly and safely Remove the specimen disc and lay it flat on the stage Remove the specimen using a single edge blade Then remove remains of cyanoacrylate adhesive from the specimen disc Remove and empty out the buffer tray Dispose of the contents of the buffer tray properly Drain the cooling bath To do so release the tube from its holder at the rear of the instrument and dispose of the contents of th...

Page 29: ...n disc sufficiently to prevent collision Audible warning signal Return stroke is not completed Operating error due to locking function of the REV FORW button With the REV FORW button locked the instrument is switched off via the power switch at the rear of the instrument and is switched on again without releasing the REV FORW button to its center position Unlock the REV FORW button by pulling it b...

Page 30: ...off via the EMERGENCY STOP foot switch or Emergency stop button and after that the EMERGENCY STOP was released without unlocking the UP DOWN button Release the UP DOWN button to its center position To reactivate the downward motion activate the UP DOWN button again DOWN The feed motor stops Any processing step sectioning stroke etc is interrupted immediately Any UP DOWN motion of the buffer tray i...

Page 31: ...tant electronic component defective Switch off the instrument call the Technical Service Error code E0 400 is displayed Feed motor defective Switch off the instrument call the Technical Service Error code E 05xx is displayed Light barrier error forward feed Switch off the instrument call the Technical Service Audible warning signal Error code E0 600 is displayed Light barrier error section thickne...

Page 32: ...ser has tried to select a specimen thickness via the button that is below the minimum value 0 μm or above the maximum value 999 μm Release the button Audible warning signal When operating the instrument for the first time or after the E EPROM has been exchanged The warning signal will cease automatically after the initialization phase A clattering sound can be heard The visible clamping screws hav...

Page 33: ... Remove the blade from the knife holder and insert it in the receptacle at the bottom of the blade dispenser Remove the knife holder for cleaning Remove the specimen plate from the buffer tray and lay it flat on the stage Carefully remove the specimen with a single edge blade Remove section waste using tweezers or a brush Remove the buffer tray empty it and rinse it separately with water p 28 5 6 ...

Page 34: ... power socket Then check the fuses at the rear side of the instrument To do so proceed as follows Using a screwdriver Fig 28 1 carefully push out the fuse insert Fig 28 2 Remove the fuse insert it contains two fuses Fig 28 3 Check that the thin wire Fig 28 4 in the glass capillary of a fuse is intact If not replace the fuse the standard scope of delivery includes two replacement fuses Warning Befo...

Page 35: ... 32060 Foot switch with protective housing 14 0463 27415 Magnifier complete 14 0462 31191 Module LED Hi Power spots 2 arm 14 6000 04826 Module Hi Power Spot LED 1000 14 6000 04825 Sapphire knife 14 0216 39372 Cyanoacrylate glue 14 0371 27414 Julabo FL300 recirculating cooler chiller 100 V 50 60 Hz 14 0481 48439 115 V 50 Hz 14 0481 48437 230 V 50 60 Hz 14 0481 48436 230 V 60 Hz 14 0481 48438 Antifr...

Page 36: ...buffer in the proper position can be added to the double walled buffer tray First connect the hoses Fig 31 1 included in the standard delivery of the double walled buffer tray to the rear of the Julabo Recirculating Cooler Chiller FL300 then connect the other end to the empty buffer tray Access is easier if you make the left connection first To do so pull back the lock coupling attach the hose and...

Page 37: ...0462 31191 Module LED Hi Power spots 2 arm To be mounted onto the magnifier after the magnifier has been mounted into the fixture Then connect the module LED Hi Power spots 2 arm to the Module Hi Power spot LED 1000 Order No 14 6000 04826 Fig 33 Module Hi Power spot LED 1000 Serves as a light source for the module LED Hi Power spots 2 arm Order No 14 6000 04825 ...

Page 38: ...n the VT1000 S and VT1200 VT1200 S Selectable temperature range 20 C to 40 C Recommended cooling medium Antifrogen N 14 0481 45443 Mixture with water 50 50 Application example If at an ambient temperature of 20 22 C a temperature of 4 C is to be reached in the buffer trough the setting value of 0 5 2 C must be selected Note For additional information refer to the Instructions for Use provided with...

Page 39: ...e warranty terms of your Leica sales organization or the organization from which you have purchased the contractual product shall apply exclusively Service information If you are in need of technical customer support or spare parts please contact your Leica representative or the Leica dealer where you purchased the instrument Please provide the following information Model name and serial number of...

Page 40: ...www LeicaBiosystems com within the product menu This template has to be used for gathering all required data When returning a product a copy of the filled and signed confirmation has to be enclosed or passed on to the service technician The responsibility for products that are sent back without this confirmation or with an incomplete confirmation lies with the sender Returned goods that are consid...

Page 41: ......

Page 42: ...www LeicaBiosystems com Version 2 4 Revision L 11 2019 Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 69226 Nussloch Germany Tel 49 0 6224 143 0 Fax 49 0 6224 143 268 Web www LeicaBiosystems com ...

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