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MultiGene Thermal Cycler

TC9600-G/ TC9600/TC6000-G/TC6000

Operation Manual

Ver.1.4

Labnet International, Inc.

Summary of Contents for TC6000

Page 1: ...MultiGene Thermal Cycler TC9600 G TC9600 TC6000 G TC6000 Operation Manual Ver 1 4 Labnet International Inc...

Page 2: ...Purchase of this product does not convey a license or right to perform patented PCR processes Applied Biosystems holds some of the basic PCR patents that may or may not be currently valid For addition...

Page 3: ...CREATING A NEW USER FOLDER 13 4 3 EDITING A USER FOLDER 13 4 4 DELETING A USER FOLDER 14 5 PROTOCOL MANAGEMENT 15 5 1 RUNNING A PROTOCOL 15 5 2 PAUSE A PROTOCOL 16 5 3 VIEWING RUNNING PROTOCOL INFORM...

Page 4: ...ING THE UNIT 29 10 2 CLEANING THE HEATED LID 29 10 3 REPLACING A FUSE 29 10 4 CHANGING THE HEATER BLOCK 29 11 TROUBLESHOOTING 30 11 1 ERROR MESSAGES 31 APPENDIX A TECHNICAL SPECIFICATIONS 32 APPENDIX...

Page 5: ...ine 5 Install the machine in the room temperature 15 30 C relative humidity 20 80 6 DO NOT block the air vents 7 Keep the side and rear of the machine at least 30cm from the wall or other machine 8 Ma...

Page 6: ...to use graphical interface Extended Temperature return function that makes touchdown possible Gradient function enables optimization of annealing temperatures Preset protocols that make starting ampli...

Page 7: ...ed when the ENTER key is pressed Alphanumeric Keys 0 9 The alphanumeric keys are usually used as number keys for the entry of time temperature password and cycle numbers When entering a user name or p...

Page 8: ...ock for better contact Rotate Lid Knob in counter clockwise direction until the Lid pops up Only after the Lid pops can users slide the Lid to the rear Sample Block Section of the unit in which sample...

Page 9: ...djustment Potentiometer Control the brightness of LCD panel Please use Philip head screw driver to adjust it USB Port For protocol or logs transfer to the computer RS 232 Port For protocol or logs tra...

Page 10: ...reen will show the software version for 10 seconds and then enter User Folder screen Pressing any key will go to User Folder screen immediately 3 3 Heated Lid The Heated Lid heats the air in the upper...

Page 11: ...d 1 Slide the lid to the rear 2 lift up the lid The first method is recommended for common operations and to prevent the skin burn from the heated lid The second method is used to clean up the heating...

Page 12: ...8 Figure 5 Slide the lid backward to access the block Figure 6 Slide the lid to align the lid hinge with notches of top case Figure 7 Lift the lid...

Page 13: ...MultiGene Thermal Cycler 9 Figure 8 Rest the lid in vertical position...

Page 14: ...ate a protocol 3 EDIT VIEW a Protocol and 4 DELETE a Protocol The function map can is shown in Figure 6 Various functions including Gradient can be added to the selected created Protocol Main Menu EDI...

Page 15: ...o 6 7 P Q R S p q r s 7 8 T U V t u v 8 9 W X Y Z w x y z 9 3 7 Loading the Reaction Vessel For the optimal performance of the MultiGene we recommend Labnet tubes and plates The recommended sample vo...

Page 16: ...cation Number PIN to enter 2 The PRESET folder includes some frequently used Protocols defined by vendor while the PUBLIC folder is open to all users 4 1 Viewing a User Folder All Protocols are stored...

Page 17: ...ng F1 ACCEPT will save the new folder name and PIN Max User folder name 12 characters Max User folder no 20 4 3 Editing a User Folder Under the User Folder screen use directional arrow keys to select...

Page 18: ...lete the selected User folder You are required to enter the PIN and press F1 ACCEPT or ENTER The screen will prompt Do you want to delete Press F1 ACCEPT to confirm the deletion Press F5 EXIT to abort...

Page 19: ...hd do ow wn n 0 07 7 2 25 5 0 06 6 0 09 9 1 10 0 2 20 0 P PR RO OT TO OC CO OL L D DA AT TE E T TI IM ME E L La ab b 0 07 7 2 28 8 0 06 6 0 03 3 2 25 5 3 30 0 R RU UN N N NE EW W E ED DI IT T E EX XI...

Page 20: ...e segment Once the countdown is completed the highlighted section will continue to move to the next temperature or cycle segment and start counting down the time 2 The Lid temperature will flash on th...

Page 21: ...72 2 0 00 0 4 4 0 00 0 5 5 0 00 0 0 0 3 30 0 0 0 3 30 0 0 0 3 30 0 1 10 0 0 00 0 T Te em mp p T Ti im me e 1 1 T Te em mp p 3 3 T Te em mp p 2 25 5 C Cy yc c 2 2 T Te em mp p 1 1 C Cy yc c R Ru un n...

Page 22: ...the original protocol settings 6 1 Changing Temperature Time Values 1 Press directional arrow keys to move cursor to the temperature or time settings required to be changed 2 Enter your desired values...

Page 23: ...t only the selected segment will be inserted deleted 6 3 Changing Temperature Segment Count Temp and Cycle Count Cyc The Temperature Segment Count Temp can be modified by entering a new value 1 to 9 i...

Page 24: ...emperature setting place your cursor in the same temperature segment with G Gradient Temperature symbol Press F2 GRAD Again The screen will show the calculated temperature gradient and High and Low te...

Page 25: ...ess ENTER The graph will show the increment decrement values 4 Press F1 ACCEPT to accept the values or F2 CLEAR to re enter the values To remove the Incremental Decremental Temperature and Time settin...

Page 26: ...0 4 4 0 0 5 5 0 00 0 0 0 3 30 0 0 0 3 30 0 0 0 3 30 0 1 10 0 0 00 0 T Te em mp p T Ti im me e 1 1 T Te em mp p 3 3 T Te em mp p 2 25 5 C Cy yc c 2 2 T Te em mp p E EX XI IT T C CL LE EA AR R T Te em m...

Page 27: ...rotocol An editing Protocol can be saved under a new name newly created Protocol or under the same name edited Protocol Under the User s Folder choose the Protocol to be edited Select F3 EDIT to edit...

Page 28: ...e deletion Note Protocols in the Preset Protocols Folder can t be deleted while Protocols in the Public Folder can be deleted by any user if not password protected M MI IK KE E P PR RO OT TO OC CO OL...

Page 29: ...e audible beep 5 Administrator Users can delete unwanted Usernames and their Protocols 6 Service Mode is reserved for Service engineers S SE EL LE EC CT T S SY YS ST TE EM M 8 8 2 21 1 2 20 00 06 6 1...

Page 30: ...tup 9 2 Date Time Users can change the date type default date and time of this unit by selecting Date Time screen as shown below under SYSTEM screen Please remember to press F1 ACCEPT to store your se...

Page 31: ...M M Y Y D De ef fa au ul lt t D Da at te e 0 08 8 2 21 1 0 06 6 D De ef fa au ul lt t T Ti im me e 1 11 1 1 17 7 4 44 4 P Pr re es ss s F F1 1 t to o s sa av ve e y yo ou ur r s se et tt ti in ng gs...

Page 32: ...o delete any User Folder and any Protocols inside a User s Folder 9 6 Service Mode Only authorized service personnel have the password to enter Service Mode and perform necessary maintenance and repai...

Page 33: ...lowering it into the tracks Once the lid in secure in the tracks make sure the lid easily slides forward 10 3 Replacing a Fuse The fuse is located in the power socket module just below the power conn...

Page 34: ...Operating temperature environment may be unsuitable 2 Electronic cooling element may be damaged or aged 1 Operate the unit in a environment temperature between 15 to 30 C 2 Return the unit for service...

Page 35: ...sage Cause Action HEATER ERROR Lid temperature sensor problem Lid temperature exceeds 132 C Lid temperature can t reach the set temperature Reboot the unit BLOCK ERROR Block temperature sensor or heat...

Page 36: ...C Max Temp Increment Decrement Rate 3 C 2 C 3 C 2 C Gradient Temperature 30 99 C Max temp difference 24 C N A 30 99 C Max temp difference 24 C N A Max Lid Temperature 100 115 C 100 115 C Protocol Cap...

Page 37: ...3 30 0 1 10 0 0 00 0 T Te em mp p T Ti im me e 1 1 T Te em mp p 2 2 T Te em mp p 2 25 5 C Cy yc c 2 2 T Te em mp p 1 1 C Cy yc c P PR RE ES SE ET T G GR RA AD DI IE EN NT T R RU UN N 8 8 2 21 1 2 20 0...

Page 38: ...l l 5 5 1 10 00 0u ul l 1 1 T Tu ub be e C Co on nt tr ro ol l 2 2 B Bl lo oc ck k C Co on nt tr ro ol l 0 0 5 5 P PR RE ES SE ET T T Ti im me e i in nc c R RU UN N 2 20 00 05 5 8 8 2 21 1 1 13 3 0 00...

Page 39: ...Thermal Cycler Model Names TC9600 G TC 9600 TC6000 G TC6000 All models comply with the following European standards EMC EN 61326 Group 1 Class B Safety EN 61010 1 and EN 61010 2 010 To the best of my...

Page 40: ...default telnet program select NO 6 Name the connection link 7 Select the com port COM3 8 Port settings a Bits sec 9600 b Parity None c Stop bits 1 b Flow control None USB Driver Installation 1 Place C...

Page 41: ...ables Pack Size Description Catalog Number Sealing Mats Tape TC 96 MAT 20 Sealing Mat 96 well Microplate TC96 AS 100 100 Thermal Tape PCR AS 200 100 Aluminum Sealing Tape Consumables Pack Size Descrip...

Page 42: ...plied adhesive provides optimum well sealing Functional Temperature range 80 C to 104 C Cat TC AS 100 Silicone Compression Mat is used to seal 96 well plates when using the MultiGene Thermal Cycler he...

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