MultiGene Thermal Cycler
21
Note: The temperature values between 2
nd
and 11
th
rows are calculated values and
represent an algorithmic based on the heat distribution though out the block.
6.4.2
Incremental/Decremental Temperature and Time
Settings
To increase or decrease the dwell time or temperature after each repeat you must have
more than one cycle repeat. i.e. 55ºC and a dwell time 40 seconds, you could decrement
the second step by 5 seconds the second step would be 55ºC and a dwell time of 35
seconds.
1. Place the cursor at the Temperature or Time values. Press
F3:INCRE.
2. To select increment/decremental values. The screen will ask to input the increment
values. Press
F3: +
or
F4: -
to choose increment or decrement.
3. Enter your desired values and press
ENTER
. The graph will show the
increment/decrement values.
4. Press
F1:ACCEPT
to accept the values or
F2:CLEAR
to re-enter the values.
To remove the Incremental/Decremental Temperature and Time settings, place the cursor
in the temperature segment with “Incremental/Decremental Temperature and Time
values”. Select
F2:CLEAR
to clear the values. Select
F1:ACCEPT
will remove the
incremental/ Decremental Temperature setting and its symbol.
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