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The accuracy and precision (repeatability) of liquid vol-
ume depend on the quality of tips used. The values for
accuracy and precision given in the table below were
obtained using 

Labnet

tips. Only those tips guarantee

correct operation of the pipettes and ensure accuracy
and precision of liquid sampling.

These specifications are obtained in forward mode,
using a gravimetric method with the temperature of the
distilled water, tips and all other conditions stabilized
between 19°C and 21°C. Number of measurements -
minimum 10. The values given include all components of
error due to both normal handwarming and the changing
of the tip.
Performance test: The pipette is calibrated in accordance
with EN ISO 8655. Performance can be verified by
checking the pipette using the procedures outlined in the
EN ISO 8655 standard.
The pipette design enables the user to perform the recalibra-
tion process according to the rules presented in section 8.

3

2 - SETTING THE VOLUME

The volume display shown by the counter has three dig-
its, which should be read from top to bottom. In addition
the lowest counter drum is printed in a scale which
enables the setting of the volume in the minimum gradua-
tion range. Examples of the meanings of the black and
red digits:

Pipettes 

BPP2

Red figures at the bottom = 1/100 μl
Increment = 0.002 μl

Pipettes 

BPP10, BPP20, 

Red figures at the bottom = 1/10 μl
Increment = 0.02 μl

Pipettes 

BBPP100, BPP200

Black figures only = 1 μl
Increment = 0.2 μl

Pipettes 

BPP1000, BPP5000, 

Red figures at the top - ml

Increment =       2 μl   

10 μl

Pipettes 

BPP10000

Red figures at the top - ml
Increment = 20 μl

The volume of the pipette is set by the knob in the pipet-
ting pushbutton (Fig. 1A2) or by the adjustment knob
(Fig. 1B). The volume adjustment can be performed
when the locking ring is set in the lower position (fig. 5A).
When the desired volume is selected, the the locking ring
should be set in the upper position (Fig.5B). To attain the
maximum accuracy, set volume must be approached
from a higher value by diminishing counter readings. 
• If the desired volume is lower than set volume shown

by the counter, the operator should turn the pipetting
pushbutton (Fig. 1A2) or the adjustment knob (Fig. 1B) 

4

Model

Volume 

[μl] 

Accuracy

[%] 

Precision 

[%]

Fit to tips

μl

BPP2

BPP10

BPP20

BPP100

BPP200

BPP1000

BPP5000

BPP10000

0.2
1.0

Max

2.0

Min

0.5
5.0

Max

10.0

Min

2

10

Max

20

Min

10
50

Max

100

Min

20

100

Max

200

Min

100
500

Max

1000

Min

1000
2500

Max

5000

Min

1000
5000

Max 10000

± 12.0
± 2.7
± 1.5
± 4.0
± 1.0
± 0.5
± 3.0
± 1.0
± 0.8
± 1.6
± 0.8
± 0.8
± 1.2
± 0.8
± 0.6
± 1.6
± 0.7
± 0.6
± 0.6
± 0.6
± 0.5
± 2.5
± 0.8
± 0.5

± 6.0
± 1.3
± 0.7
± 2.8
± 0.6
± 0.4
± 1.5
± 0.5
± 0.3
± 0.80
± 0.24
± 0.20
± 0.60
± 0.25
± 0.20
± 0.40
± 0.20
± 0.15
± 0.25
± 0.20
± 0.15
± 0.6
± 0.3
± 0.2

10

200

1000

5000

10000

BPP2

BPP10

BPP20

BPP100

BPP200

BPP1000

BPP10000

BPP5000

Summary of Contents for BioPette PLUS BPP10

Page 1: ......

Page 2: ...A N F G J I C A1 A2 B P C D E D L A B C D E F ...

Page 3: ...P 5A 5B P 5 4 6 4D 4A 4B 4C 6A 2 1000 µl 7 6B 5000 µl 10000 µl 6C 5000 µl 10000 µl D D D D D D ...

Page 4: ...PLUS is equipped with a built in tip ejector Fig 1D The construction of the ejector enables the user to adjust the length This allows for proper fit and ejection of nearly every tip type available When using narrow tubes it may be necessary to remove the tip ejector It is simply removed by pulling down Adjusting of the Tip Ejector Length in 2 1000 μl pipettes Fig 6A The adjusting H spacers include...

Page 5: ...ed figures at the bottom 1 100 μl Increment 0 002 μl Pipettes BPP10 BPP20 Red figures at the bottom 1 10 μl Increment 0 02 μl Pipettes BBPP100 BPP200 Black figures only 1 μl Increment 0 2 μl Pipettes BPP1000 BPP5000 Red figures at the top ml Increment 2 μl 10 μl Pipettes BPP10000 Red figures at the top ml Increment 20 μl The volume of the pipette is set by the knob in the pipet ting pushbutton Fig...

Page 6: ...touching the orifice of the tip Dispensing Place the end of the tip against the inside wall of the vessel at an angle of 10 to 40 degrees Press the pushbutton smoothly to the first stop Fig 2C Wait one second Press the pushbutton to the second stop to expel any remaining liquid Fig 2D Keeping the pushbutton depressed to the very end remove the pipette by drawing the tip against the inside surface ...

Page 7: ... used for volumes between 0 1 μl and 10 μl They are used with the BPP2 and the BPP10 models Tips 200 These tips are used for volumes between 2 μl and 200 μl They are used with the BPP20 BPP100 and the BPP200 models which are equipped with the yellow label on the pushbutton γ m m γ 7 Tips 1000 These tips are used for volumes between 100 μl and 1000 μl They are used with the BPP1000 models which are...

Page 8: ...20 25 C and stabilized during weighing within 0 5 C Measurements should be conducted using distilled water Balance sensitivity should be suitable for the volume to be controlled 10 Recalibration procedure Set the dose volume depending on the pipette volume according to the following table Perform 5 aspirations weigh each one and calculate the average value of the aspirations Calculate average aspi...

Page 9: ...of the shaft is cracked or scored Remove the tip ejector Unscrew the shaft nut inspect the shaft and the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened In the models BPP2 BPP10 and BPP20 the damage of the shaft may also cause a damage of the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette ...

Page 10: ... an autoclave at 121 C for 20 minutes After sterilization the pipette should be dried and cooled to room temperature It is recommended to sterilize the pipettes in autoclave with an initial vacu um and drying cycle prior to sterilization unscrew the shaft nut slightly in the BPP2 BPP1000 pipettes and unscrew the shaft slightly in the BPP5000 and BPP10000 After autoclaving these parts should be scr...

Page 11: ...Before returning a pipette to a Labnet representative please ensure that the pipette is completely free of any contamination chemical microbial or radioactive Contact Labnet at 1 732 417 0700 fax 1 732 417 1750 email labnet labnetlink com Warning The replacement of the piston requires con ducting of calibration procedure according to section 8 BPP 04 2010 All rights reserved Product described in t...

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