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CONTENTS 

1 - INTRODUCTlON
2 - SETTING THE VOLUME 
3 - ASPIRATING AND DISPENSING LIQUIDS
4 - PRE-RINSING
5 - DENSE AND VISCOUS LIQUIDS 
6 - LABNET PIPETTE TIPS
7 - RECOMMENDATIONS 
8 - RECALIBRATION
9 - TROUBLESHOOTING 

10 - CLEANING AND STERILIZATION
11 - PIPETTE KIT AND ACCESSORIES
12 - SPARE PARTS

1 - INTRODUCTION

The 

BIOPETTE PLUS

is a volumetric instrument de-

signed to measure and transfer liquids precisely and
safely. It can measure and transfer volumes from 0.1 μl
to 10000 μl depending on the model.
The 

BIOPETTE PLUS

pipettes are equipped with a digi-

tal counter which shows the pipetting volume. 
The set volume is visible in the window on the handle.
The setting of the volume is done by turning the pipetting
pushbutton knob (Fig. 1A2) or by turning the adjustment
knob (Fig. 1B) in the correct direction. The pipette
design allows the user to lock the volume setting by
pushing the locking ring upwards (Fig.1S). The position
of the ring is indicated by the symbols located on the
handle. The volume range of the aspired liquid is shown
on the pipetting pushbutton (Fig. 1A1).
The 

BIOPETTE PLUS

pipettes are produced in 8 models,

covering the volume range from 0.1 μl to 10000 μl.

1

The liquid is aspirated into disposable tips attached to
the pipette shaft. Disposable tips ensure maximum safety
and eliminate possibility of crosscontamination of the liquid
samples.
To protect the user from contaminated tips the 

BIOPETTE

PLUS

is equipped with a built-in tip ejector, (Fig. 1D).

The construction of the ejector enables the user to adjust
the length. This allows for proper fit and ejection of nearly
every tip type available. When using narrow tubes, it may
be necessary to remove the tip ejector. It is simply
removed by pulling down.

Adjusting of the Tip Ejector Length

- in 2-1000 μl pipettes (Fig. 6A). 

The adjusting ”H” spacers, included in the box allow for
adjusting the length of tip ejector by +1mm or +2mm.
An ”H0” spacer is inserted on a standard basis. The out-
side shape of the spacer identifies the size change.

- in 5000 and 10000 μl pipettes (Fig. 6B).

The length of tip ejector is adjusted by screwing in or
screwing out the tip ejector stem with a screwdriver. Turn
the screwdriver counter clockwise to increase the length
of tip ejector, and clockwise to reduce the length of tip
ejector. The ejector can be adjusted in the range of 5 mm.
If above described method of ejector adjustment is not
sufficient or the diameter of the ejector opening is too
large to eject the tip it is necessary to put the ejector cap
“M” onto the ejector, (Fig. 6C).
The 

BIOPETTE PLUS

is a high quality instrument which

offers excellent accuracy and precision.

2

BPP2, BPP10

BPP20, BPP100, 
BPP200, BPP1000

BPP5000,BPP10000

Measurement and transfer of micro-volumes,
DNA sequencing and enzyme-assay applications.

Measurement and transfer of general 
aqueous solution, acids and bases

Measurement and transfer of large volumes.

Model 

BPP2

BPP10

BPP20

BPP100

BPP200

BPP1000

BPP5000

BPP10000

Volume range [μl]

0.1 - 2

0.5 - 10

2 - 20

10 - 100

20 - 200

100 - 1000

1000 - 5000

1000 - 10000

Summary of Contents for BioPette PLUS BPP10

Page 1: ......

Page 2: ...A N F G J I C A1 A2 B P C D E D L A B C D E F ...

Page 3: ...P 5A 5B P 5 4 6 4D 4A 4B 4C 6A 2 1000 µl 7 6B 5000 µl 10000 µl 6C 5000 µl 10000 µl D D D D D D ...

Page 4: ...PLUS is equipped with a built in tip ejector Fig 1D The construction of the ejector enables the user to adjust the length This allows for proper fit and ejection of nearly every tip type available When using narrow tubes it may be necessary to remove the tip ejector It is simply removed by pulling down Adjusting of the Tip Ejector Length in 2 1000 μl pipettes Fig 6A The adjusting H spacers include...

Page 5: ...ed figures at the bottom 1 100 μl Increment 0 002 μl Pipettes BPP10 BPP20 Red figures at the bottom 1 10 μl Increment 0 02 μl Pipettes BBPP100 BPP200 Black figures only 1 μl Increment 0 2 μl Pipettes BPP1000 BPP5000 Red figures at the top ml Increment 2 μl 10 μl Pipettes BPP10000 Red figures at the top ml Increment 20 μl The volume of the pipette is set by the knob in the pipet ting pushbutton Fig...

Page 6: ...touching the orifice of the tip Dispensing Place the end of the tip against the inside wall of the vessel at an angle of 10 to 40 degrees Press the pushbutton smoothly to the first stop Fig 2C Wait one second Press the pushbutton to the second stop to expel any remaining liquid Fig 2D Keeping the pushbutton depressed to the very end remove the pipette by drawing the tip against the inside surface ...

Page 7: ... used for volumes between 0 1 μl and 10 μl They are used with the BPP2 and the BPP10 models Tips 200 These tips are used for volumes between 2 μl and 200 μl They are used with the BPP20 BPP100 and the BPP200 models which are equipped with the yellow label on the pushbutton γ m m γ 7 Tips 1000 These tips are used for volumes between 100 μl and 1000 μl They are used with the BPP1000 models which are...

Page 8: ...20 25 C and stabilized during weighing within 0 5 C Measurements should be conducted using distilled water Balance sensitivity should be suitable for the volume to be controlled 10 Recalibration procedure Set the dose volume depending on the pipette volume according to the following table Perform 5 aspirations weigh each one and calculate the average value of the aspirations Calculate average aspi...

Page 9: ...of the shaft is cracked or scored Remove the tip ejector Unscrew the shaft nut inspect the shaft and the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette the nut should be hand tightened In the models BPP2 BPP10 and BPP20 the damage of the shaft may also cause a damage of the piston assembly Replace the damaged parts see Section 12 When reasembling the pipette ...

Page 10: ... an autoclave at 121 C for 20 minutes After sterilization the pipette should be dried and cooled to room temperature It is recommended to sterilize the pipettes in autoclave with an initial vacu um and drying cycle prior to sterilization unscrew the shaft nut slightly in the BPP2 BPP1000 pipettes and unscrew the shaft slightly in the BPP5000 and BPP10000 After autoclaving these parts should be scr...

Page 11: ...Before returning a pipette to a Labnet representative please ensure that the pipette is completely free of any contamination chemical microbial or radioactive Contact Labnet at 1 732 417 0700 fax 1 732 417 1750 email labnet labnetlink com Warning The replacement of the piston requires con ducting of calibration procedure according to section 8 BPP 04 2010 All rights reserved Product described in t...

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