Jasco P-1010, Hardware/Function Manual, Page: 25 / 29

13
the cell in the same position in the cell holder facing the same direction.
Measurement is automatically performed and standard sample data is printed.
3) Next, remove the sample from the sample chamber. Place it back in the
sample chamber 5 times to confirm repeatability of the data.
<Acceptance criteria>
At a temperature of 20 - 30
°
C, the data must be within the range of +3.325
±
0.007
°
.
The results of repeated measurements must also be within this range.
Note: Acceptance criterion of P-1010 corresponds to the expected value in the Test
Certificate for interference filter. This expected value is calculated for a 10mm
cell. Also the measurement value deteriorates depending on the change in
the transmittance of the filter.
[Reference material]
The reference values for specific optical rotations of aqueous sucrose solution are
listed below.
Table 7.1 Specific optical rotation (deg) of Aqueous Sucrose Solution (20
°
C)
[
α
]
λ
= 21.648 / (
λ
2
- 0.0213) :C = 26
Line
[
α ]obs
[
α ]calc
[ α ]obs
-[ α ]cal Line
[
α ]obs
[
α ]calc
[ α ]obs
-[ α ]calc
Degrees Degrees Degrees Degrees Degrees Degrees
Li 6708
Cd 6438 50.51
55.04 50.50
55.05 +0.01
-0.01 Fe 4384
*
Fe 4376
*
126.5
*
127.2
*
126.7
127.2 +0.12
±
Zn 6362 56.51 56.45 +0.06 Hg 4358 128.49 128.3 +0.12
Na 5893
Cu 5782
Hg 5780
Cu 5700
Hg 5461
Cu 5218
Cu 5153
Cu 5106
Cd 5086
Zn 4811
Cd 4800 66.45
69.10
69.22
71.24
78.16
86.21
88.68
90.46
91.16
103.07
103.62 66.44
69.16
69.21
71.30
78.18
86.25
88.63
90.44
91.20
103.03
103.53 +0.01
-0.06
+0.01
-0.06
-0.02
-0.04
+0.05
+0.02
-0.04
+0.04
+0.09 Fe 4353
*
Fe 4337
*
Fe 4315
*
Fe 4282
*
Fe 4272
*
Fe 4261
*
Fe 4191
*
Fe 4144
*
Fe 3889
*
Fe 3833
*
Fe 3826
*
128.5
*
129.8
*
130.7
*
133.6
*
134.2
*
134.9
*
140.0
*
144.2
*
166.7
*
171.8
*
173.1
*
128.7
129.8
131.3
133.6
134.3
135.1
140.2
143.9
166.7
172.3
173.2 -0.2
±
-0.6
±
-0.1
-0.2
-0.2
+0.3
±
-0.5
-0.1
**
Zn 4722
Zn 4680
Cd 4678
107.38
109.49
109.69
107.33
109.48
109.48
+0.05
+0.01
+0.11
* The numbers of the portion indicated are read values of light measured by using
the photographic method.
T.M. Lowry
：
"Optical Rotatory Power ",P131 Longmans,Green & Co. (1935)
** Due to a misprint in the above, the values were calculated by our company.
Handbook of Chemistry and Physics, 42nd Edition (1960 - 1961) P3019
[
α
]
20
D = 66.412 + 0.01267
･ d - 0.00376 ･ d
2
： ( d = 0
∼
50 )
α
t
D =
α
20
D { 1 - 0.00037 ( t - 20 )}
：
( t = 14
～
30
°
C )
National Bureau of Standards
[
α
]
20
D = 66.529
：
( C = 26 )