HiSeq v4 chemistry 11
performed vs. entered 15, 32
split reagent kits 53
TruSeq v3 chemistry 26
O
online support 1
optics module 2
orange flow cell lever 49
output folders
P
pause options 52
PE reagent positions 18, 42
performance specifications 11, 26
phasing 58-59
PhiX alignment 15, 31
Phred algorithm 59
positioning flow cells 19, 21, 35, 38
positions, reagents
indexing 17, 34
PE 18, 42
SBS 16, 33
possible cycles
HiSeq v4 chemistry 11
TruSeq v3 chemistry 26
post-run wash 24, 43
preparing reagents, TruSeq v3 workflow 27
prephasing 58
preventive maintenance 45
priming flow cell 18, 35
priming preparation 20, 36
priming waste 20, 36
Q
Q-scores 59
quality scores
quality tables 59
R
racks, reagent 3
reagent chiller, temperature 3
reagent kit ID, recording 15, 32
reagent kits, catalog numbers 5
reagent positions
PE rack 17-18, 34, 42
SBS rack 16, 33
reagent racks 3
reagent volumes, split SBS kits 53-54
reagents
changing midrun 52
handling post-run 23, 43
shorter runs 53
reagents, SBS
creating 2 sets 53
TruSeq v3 workflow 27
reagents, storing split kits 53-54
recipes, custom 15, 32
recording flow cell IDs 14
recording reagent kit IDs 15, 32
registration, troubleshooting 57
rehybridization 54
remote monitoring 14, 30
reports, first base incorporation 23, 39
restarting the instrument 48
resume options 50
resuming runs, settings 51
reusing maintenance wash solution 46
RTA 4, 56
input files 56
resuming 51
stop options 51
stopped runs 50, 57
run folder location 62
run folder size 5
run folders, temporary 62
run information file 61
run metrics
availability by cycle 59
viewing 23, 39
Run Overview screen 23, 39
run parameters, reviewing 16, 33
S
sample sheet 60
sample sheets, requiring 15, 32
SAV 4
documentation 23, 40
InterOp files 61
version 56
saving thumbnail images 14, 31
SBS reagent positions 16, 33
sensors 5
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
66
HiSeq 2000 System Guide