Figure 3
Reagent Compartment
A
Sipper handles
B
Reagent rack for indexing and paired-end reagents
C
Reagent rack for SBS reagents for flow cell A
D
Reagent rack for SBS reagents for flow cell B
HiSeq 2000 Software
Three software applications are installed on the instrument computer:
u
HiSeq 2000 control software—The HiSeq Control Software (HCS) interface guides you through the steps
to set up a sequencing run. During the run, the control software operates instrument hardware, controls
fluidics, sets temperatures, and provides a visual summary of quality statistics.
u
Real-Time Analysis software—Integrated with the control software, Real-Time Analysis (RTA) performs
base calling and assigns a quality score to each base for each cycle. For more information, see
.
u
Sequencing Analysis Viewer software—Sequencing Analysis Viewer (SAV) provides detailed quality
statistics.
Status Icons
A status icon located in the upper-right corner of each screen shows changes in conditions, errors, or
warnings during run setup and during the run.
Status
Icon
Status
Name
Description
Status okay
No change. System is normal.
Information
Information only. No action is required
Attention
Information that might require attention.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
4
HiSeq 2000 System Guide