These volumes result in a solution that is approximately 0.5% Tween 20 and 0.03% ProClin 300.
6
Continue stirring until thoroughly mixed.
7
Set aside in a closed container at room temperature.
Tween 20 and ProClin 300
1
From the Welcome screen, select Wash | Maintenance.
2
If a run included an indexing read or paired-end turn, select Yes to wash PE reagent positions.
Otherwise, select No.
3
Select Next.
4
If you are using fresh maintenance wash solution, load the instrument with solution as follows.
a
Fill eight SBS bottles with 250 ml fresh wash solution.
b
Fill 10 PE tubes with 12 ml fresh wash solution.
c
Assign each bottle and tube to a reagent rack position. Maintain these assignments for each
subsequent wash to prevent cross-contamination from reagent present on the sippers.
5
If you stored maintenance wash solution from a previous run, load the instrument with solution as follows.
a
Replenish the stored solution and invert to mix. Replenish no more than two times after the original
use.
b
Load the bottles and tubes into the assigned reagent rack positions.
NOTE
Monthly replacement of wash bottles and tubes is typically sufficient.
6
Empty the waste bottle.
7
Select the Wash solution loaded and template loading station closed checkbox.
8
Select Next.
9
Remove the flow cell from the flow cell stage and set it aside.
10 Put on a new pair of powder-free latex gloves.
11 Apply light pressure to one side of the front gasket until the other side lifts. Use tweezers to grasp and
remove the gasket. Repeat to remove the rear gasket.
Figure 17
Remove Used Manifold Gaskets
12 Place a new 8-port gasket in each slot on the front end and back end of the flow cell holder. Press lightly
into position.
Document # 15011190 v03
For Research Use Only. Not for use in diagnostic procedures.
46
HiSeq 2000 System Guide