illumina cBot, System Manual

Page: 41 / 90

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cBot 2 System Guide
35
Load the Manifold
From the Manifold screen, load the manifold from the same cluster kit as the flow cell.
1 Inspect the sippers on the sipper comb for damage. Make sure that the black rubber
gaskets are evenly seated.
2 Position the manifold over the flow cell with the sipper comb pointing toward the front
of the cBot.
3 Align the manifold with the guide pins on the thermal stage and seat the manifold
over the flow cell. Seat evenly to form a tight seal.
4 Select the Manifold seated over flow cell checkbox.
5 Close the flow cell clamp to secure the manifold.
Figure 13 Close the Flow Cell Clamp
6 Select the Flow cell clamp closed checkbox.
7 Connect the outlet end of the manifold to the outlet port in the wash reservoir. Make
sure that the outlet is evenly seated.
Figure 14 Secure Outlet End
A Outlet Port
B Output Clamp
8 Snap the outlet clamp closed to secure the outlet end of the manifold.
9 Select the Outlet clamp closed checkbox.
10 Align the sipper comb with the 2 metal guide pins on the front of the thermal stage.