illumina cBot, System Manual

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Material # 20004364
Document # 15065681 v01
Prepare the ExAmp Reaction
Prepare the ExAmp reaction master mix immediately before use. Follow the appropriate
instructions for the number of flow cells you are preparing.
ExAmp Reaction for 1 Flow Cell (Single-Pack Kit)
1 Invert EPX1 and EPX2 to mix.
2 Briefly centrifuge EPX1, EPX2, and EPX3.
3 Prepare the ExAmp master mix in a 1.5 ml tube as follows.
a Add 210 µl EPX1.
b Add 30 µl EPX2. Slowly pipette to mix.
c Add 110 µl EPX3. Slowly pipette to mix. Make sure that the bottom of the tube is
free of bubbles.
4 Add 35 µl master mix to the bottom of each well of the 8-tube strip. Slowly pipette to
mix. Change tips between samples.
5 Briefly centrifuge, and then set aside on ice until you are ready to load the cBot.
CAUTION
Load the 8-tube strip onto the cBot within 15 minutes .
ExAmp Reaction for 4 Flow Cells (10-Pack Kit)
1 Invert EPX1M and EPX2M to mix.
2 Briefly centrifuge EPX1M, EPX2M, and EPX3M.
3 Add the following volumes to a 1.5 ml tube in the order listed:
a Add 756 µl EPX1M.
b Add 108 µl EPX2M. Slowly pipette to mix.
c Add 396 µl EPX3M. Slowly pipette to mix. Make sure that the bottom of the tube is
free of bubbles.
4 Add 35 µl of the master mix into the bottom of each well of the 8-tube strips. Slowly
pipette to mix. Change tips between samples.
5 Briefly centrifuge the 8-tube strip, and then set aside on ice until you are ready to load
the cBot.
CAUTION
Load the ExAmp master mix and library solution onto the cBot within 15 minutes .