ibidi 12170, Instruction Manual

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Objective Heater Universal – Silver Line
2 Intended Use
The Objective Heater Universal – Silver Line is designed for use in combination with a stage top
incubation system to keep cells on a microscope stage at 37°C. The Objective Heater, operated with
the ibidi Temperature Controller – Silver Line, is a heating device for microscope objectives used
for live cell imaging. Heating of the microscope objective with the Objective Heater prevents strong
temperature gradients between the sample and the objective. This is especially important for high
magnification objectives that are in close contact with the sample (e.g., water or oil immersion objec-
tives). Using the Objective Heater improves keeping a defined and stable temperature of the sample.
Please note that the Objective Heater is designed for research purposes only.
3 Principle
Microscope objectives that require close approximation to the sample (e.g., water and oil immersion
objectives) might cause cooling of the sample by thermal transfer from the sample to the objective,
when the objective is not heated. Adequate heating of the objective by the Objective Heater prevents
cooling of the sample. The Objective Heater includes a flexible heating element that can be easily
wrapped around microscope objectives and fixed by a Velcro
®
fastener to enable efficient heating by
a large contact area between the microscope objective and the Objective Heater.
Figure 1: Schematic illustration of a live cell imaging setup with an ibidi Stage Top Incubator and the
Objective Heater for optimal temperature stability of the sample.
Version 1.0 (June 1, 2022) 12 Instruction Manual