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ibidi 12150, Instruction Manual

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ibidi Heating System Multiwell Plate – Silver Line

Contents

1

Preamble

6

1.1

Introduction

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6

1.2

Safety Symbols

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6

1.3

Nomenclature

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7

1.4

Specifications

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7

1.5

Disclaimer

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1.6

Safety Considerations

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1.7

Limited Warranty

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11

1.8

Transporting the ibidi Heating System Multiwell Plate

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11

1.9

Repairing the ibidi Heating System Multiwell Plate

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12

1.10 Waste Disposal – WEEE/RoHS Compliance Statement

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12

1.10.1 EU Directive WEEE

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12

1.10.2 EU Directive RoHS

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1.11 Regulatory Statement

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2

Intended Use

14

3

Principle

14

4

Equipment

16

4.1

Components of the ibidi Heating System Multiwell Plate

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16

4.2

Stage Top Incubator Overview

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17

4.3

ibidi Temperature Controller – Silver Line

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4.4

Heated Plate

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4.5

Heated Lid

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4.6

Cable Clip for Optional Use

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Version 1.0 (August 1, 2022)

4

Instruction Manual

Summary of Contents for 12150

Page 1: ...bidi Heating System Multiwell Plate Silver Line Version 1 0 com 12150 ibidi Heating System Multiwell Plate Silver Line 1 3 4 6 2 5 ibidiTemperature Controller Plate I 38 0 S 38 0 Run Lid Glass 42 1 42 0 36 9 37 0 Setup N C ...

Page 2: ...ibidi Heating System Multiwell Plate Silver Line Version 1 0 August 1 2022 2 Instruction Manual ...

Page 3: ...ltiwell Plate Silver Line Contact ibidi GmbH Lochhamer Schlag 11 82166 Gra felfing Germany Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E mail info ibidi de Internet ibidi com Instruction Manual 3 Version 1 0 August 1 2022 ...

Page 4: ...the ibidi Heating System Multiwell Plate 12 1 10 Waste Disposal WEEE RoHS Compliance Statement 12 1 10 1 EU Directive WEEE 12 1 10 2 EU Directive RoHS 12 1 11 Regulatory Statement 13 2 Intended Use 14 3 Principle 14 4 Equipment 16 4 1 Components of the ibidi Heating System Multiwell Plate 16 4 2 Stage Top Incubator Overview 17 4 3 ibidi Temperature Controller Silver Line 18 4 4 Heated Plate 20 4 5...

Page 5: ... 3 2 Incubation Parameters 25 5 3 3 Select Temperature Sensor 26 5 3 4 Setup Menu 26 5 4 Temperature Adjustment in the Sample 27 5 5 Sample Preparation 29 6 IncuControl Software 30 7 Maintenance 31 7 1 Disinfection and Cleaning 31 7 2 Influence of Ambient Temperature and Ventilation 31 8 Troubleshooting 32 8 1 Focus not Stable 32 8 2 Evaporation too High 32 8 3 Condensation Inside the Stage Top In...

Page 6: ...Safety Symbols Note that the signal words WARNING CAUTION and NOTE have specific meanings in this man ual Do not proceed beyond a signal word until you have performed the indicated actions Warning messages in the text are displayed in a gray shaded box Please see section 1 6 for general safety considerations WARNING A potentially hazardous situation which if not avoided could result in serious inj...

Page 7: ...e specifications given below Table 1 Specifications of the ibidi Heating System Multiwell Plate Electrical Specifications Power Supply Protection class I International protection marking IEC 60529 IP 20 Overvoltage category II Pollution degree 2 Input voltage and current AC 100 240 V 50 60 Hz 2 A 36 40 W Output voltage and current DC 24 V 6 67 A 160 W Electrical Specifications ibidi Temperature Co...

Page 8: ...Heated Plate assembled with Height 30 mm Heated Lid Weight 430 g 0 95 lbs USB cable Length 1 8 m Power supply cable Length 2 0 m power supply to wall Length 1 2 m power supply to device Temperature Control Range Heated Lid 5 degree above ambient temperature min 18 C to 45 C Heated Plate 5 degree above ambient temperature min 18 C to 45 C Heated Glass Bottom 5 degree above ambient temperature min 1...

Page 9: ...ble either directly or indirectly for any damage incurred as a result of product use The contents of this manual are subject to change without notice for product improvement This manual is considered complete and accurate at publication This manual does not guarantee the validity of any patent rights or other rights If an ibidi software program doesn t function properly this may be caused by a con...

Page 10: ... ibidi Heating System Multiwell Plate must be installed in a manner such that none of its components hinders access to the external power supply Immediately replace damaged cords plugs or cables to avoid risk of personal injury or damage to the instrument Only ibidi technical staff and technical staff instructed by ibidi are permitted to open and ser vice the ibidi Heating System Multiwell Plate T...

Page 11: ...on or misuse 4 Use of unauthorized tubing or fluidic connectors 5 Use of consumables disposables and parts not supplied by an authorized ibidi distributor 6 Corrosion due to the use of improper solvents samples or due to surrounding gases 7 Accidents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tubes fluidic co...

Page 12: ...ous Substances RoHS 1 10 1 EU Directive WEEE The ibidi Heating System Multiwell Plate must be disposed of in compliance with the WEEE Direc tive 2012 19 EC This symbol on the product is in accordance with the European Union s Waste Electrical and Elec tronic Equipment WEEE Directive The symbol indicates that this product must be recycled dis posed of separately from other household waste It is the...

Page 13: ...claration Laborgerät laboratory equipment ibidi Temperature Controller ibiTC3 XXX with ibidi accessories Der oben beschriebene Gegenstand erfüllt die Vorschriften der Richtlinie 2011 65 EU vom 08 Juni 2011 zur Beschränkung der Verwendung bestimmter gefährlicher Stoffe in Elektro und Elektronikgeräten The object of the declaration described above is in conformity with Directive 2011 65 EU of 8 June...

Page 14: ...It is not intended for use in diag nostic procedures 3 Principle Physiological Conditions in Live Cell Imaging Cells react sensitively to changes in their environment Factors such as temperature humidity and CO2 O2 levels significantly influence the outcome of cell culture assays In order to achieve biologi cally relevant and reproducible results it is crucial to maintain optimal conditions on the...

Page 15: ...experiment enabling the user to perform hypoxia experiments CO2 O2 version only The Heated Lid and Heated Plate with the Heated Glass Bottom are designed to keep cells on stage in a multiwell plate at 37 C The Heated Plate is mounted on the microscope stage The multiwell plate is placed directly on the Heated Glass Bottom and the Heated Lid is put on top The gas mixture is streamed into the chambe...

Page 16: ...n Condensation Heated Glass Bottom Heated Plate 34 C Heated Glass Bottom Heated Plate 40 C Heated Lid Unheated Lid 37 C 37 C Figure 4 The Heated Lid prevents condensation effects on the lid of the cell culture vessel Version 1 0 August 1 2022 16 Instruction Manual ...

Page 17: ...roller 1 Plate I 38 0 S 38 0 Run Lid 42 1 42 0 Setup E N C Heated Lid with electrical cable to the converter box Heated Plate with Heated Glass Bottom and electrical cable to the converter box Converter box connected to the cables of the Heated Plate and Heated Lid with multipole connectors to the ibidi Temperature Controller Silver Line Temperature Adjustment Set 1 temperature sensor 1 perforated...

Page 18: ...olumn Your inverted microscope Existing stage or frame with K frame fitting 160 x 110 mm2 on your microscope ibidi Gas Mixer Flow I 9 9 S 10 0 Run CO2 Hum 5 1 5 0 75 2 80 0 Setup 1 3 4 6 2 5 ibidi Temperature Controller 1 Plate I 38 0 S 38 0 Run Lid 42 1 42 0 Setup 37 2 Heated Plate with Heated Glass Bottom Heated Lid ibidi µ Plate multiwell plate with a ANSI SLAS SBS standard format 85 5 x 127 5 ...

Page 19: ...h to control the Temperature Controller via PC use the IncuControl software Section 6 1 3 4 6 2 5 ibidiTemperature Controller Plate I 38 0 S 38 0 Run Lid Glass 42 1 36 9 42 0 37 0 Setup N C 1 Figure 6 Front view of the ibidi Temperature Controller Silver Line The LEDs of the front panel indicate the status of the channels connection to power supply and USB connection Figure 6 Control LEDs Channel ...

Page 20: ...Ports to heating devices On Off Figure 7 Rear view of the ibidi Temperature Controller Silver Line 4 4 Heated Plate The Heated Plate provides the base for the ibidi Heating System Multiwell Plate The lower part of the Heated Plate fits into a mechanical or scanning stage with opening for mounting frames K 160 mm 110 mm Electrical connection Temperature Controller Heated Plate and Heated Glass Bott...

Page 21: ... of the lid use con densers with a working distance of 26 mm The upper glass part of the Heated Lid is electrically heat controlled The Heated Lid fits exactly and securely onto the Heated Plate For gas incubation the Heated Lid is equipped with inlets for the gas flow and the humidity sensor Detailed information on the Gas Incubation System is given in the separate instructions Luer Lock connecto...

Page 22: ... the optimal position of the clip on your stage before removing the protective foil from the self adhesive tape and mounting the clip to the stage by slight pressure It takes about one hour until full adhesion strength of the tape has been reached Open the clamp and introduce all cables and tubing leading from the Incubation Chamber to the controller s If there is no more need for the clamp it can...

Page 23: ...nected as shown in Figure 12 ibidi Gas Mixer ibidi Temp Controller Heated Tubing Package Electrical cable Gas tubing Computer optional USB cable Temperature Controller cable Humidity sensor cable Incubation Chamber Figure 12 Installation of the components of the ibidi Heating System Multiwell Plate 1 Place the ibidi Temperature Controller Silver Line next to the microscope and connect the power su...

Page 24: ...erside of the glass plate Do not touch the underside or contact it with anything conductive This could cause a short circuit that may destroy the controller and or the lid If you are not sure which temperature is set e g when operating the system the first time it is recommended to check the settings without your sample 5 3 Setting Parameters in the Front Display All control parameters can be manu...

Page 25: ... Chamber 4 Slides Channel 4 5 and 6 Plate Lid Glass or Incubation Chamber Multiwell Plate 4 Channel 6 Obj Objective Heater Universal The first three lines of the display show the respective set S set and actual I is temper ature values of the corresponding components Figure 15 If a component of a heating unit is not connected properly N C will be shown in the I value line The bottom line shows the...

Page 26: ...lect temperature sensor A No temperature sensor is selected B Select the thermo couple type K TC K as the temperature sensor by using the set buttons 5 3 4 Setup Menu The Setup menu offers the possibility to change the settings of the individual channels and the dis play For standard operation no changes need to be made Enter the setup menu by navigating to the Setup entry and pressing the round b...

Page 27: ...ibiTC30018 FW 3 1 1 TC Figure 20 Info display 5 4 Temperature Adjustment in the Sample The ibidi Temperature Controller Silver Line controls the temperature of the actively heated com ponents The temperature in the sample is affected primarily by the temperature of the Heated Plate and the Heated Glass Bottom and due to heat loss to the surrounding environment they must usu ally be set to a temper...

Page 28: ...ace the multiwell plate onto the Heated Plate 6 Close the Incubation Chamber with the Heated Lid 7 After 30 minutes check the temperature of the external sensor on the display or in the Incu Control software If the sample temperature is still too low raise the set values for the Heated Plate and Heated Lid for 0 5 1 C each and wait until the sample temperature is stable again at least 10 15 min 8 ...

Page 29: ...d Plate of the ibidi Heating System Multiwell Plate Prepare the cells according to your protocol and place the multiwell plate into the Heated Plate mounted on the microscope stage Close the Heated Lid NOTE Let the temperature of the system equilibrate for a minimum of 30 min before you start your experiments NOTE Working with high aperture objectives might limit the observation area due to their ...

Page 30: ...ownloaded from the ibidi website For more details please refer to the IncuControl instructions Figure 21 Temperature control window in IncuControl Prohibitions on the use of ibidi software The following actions are prohibited Copying software for other than backup purposes Transferring or licensing of the right to use software to a third party Disclosure of confidential information regarding softw...

Page 31: ...lvents may remove the instrument s paint To clean the heated components and or the Temperature Controller switch off the ibidi Temperature Controller Silver Line and disconnect all electrical cables Leave the instrument to cool down for approx 5 minutes WARNING Hot surface max 55 C Do not touch Heated Lid Heated Plate and Heated Glass Bottom when hot 7 2 Influence of Ambient Temperature and Ventil...

Page 32: ...y Additionally we suggest using silicone oil e g Anti Evaporation Oil ibidi 50051 to decrease evaporation Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equilibrate oil and medium inside the incubator overnight This step helps to avoid the formation of air bu...

Page 33: ...ibidi Heating System Multiwell Plate Silver Line Instruction Manual 33 Version 1 0 August 1 2022 ...

Page 34: ...ibidi Heating System Multiwell Plate Silver Line Version 1 0 August 1 2022 34 Instruction Manual ...

Page 35: ...ibidi Heating System Multiwell Plate Silver Line Instruction Manual 35 Version 1 0 August 1 2022 ...

Page 36: ...chhamer Schlag 11 82166 Gräfelfing Germany Toll free within Germany Phone 0800 00 11 11 28 Fax 0800 00 11 11 29 International calls Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E Mail info ibidi com ibidi com com ...

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