ibidi Heating System Multiwell Plate – Silver Line
Table 5: Recommended operation parameters
Parameter
Recommended Values
Heated Lid
42°C
Heated Plate
38°C
Heated Glass Bottom
37°C
To measure and adjust the sample temperature, use the provided Temperature Adjustment Set (tem-
perature sensor and culture vessel with perforated lid). The temperature of the heated components
has to be adjusted in small steps. Follow this procedure to adjust the sample temperature:
1. Set all your peripheral experimental parameters, such as room temperature, air conditioning,
airflow, illumination, microscope settings, etc.
2. Connect the external temperature sensor (thermocouple type K) to the plug at the back of the
ibidi Temperature Controller – Silver Line. The connected external temperature sensor will
appear in the display.
3. Fill the provided culture vessel (multiwell plate) with water or medium. Volumes should be
the same amount you need for your experiments.
4. Put the loose end of the thermocouple through the hole in the lid of the culture vessel and make
sure it dips into the liquid.
5. Place the multiwell plate onto the Heated Plate.
6. Close the Incubation Chamber with the Heated Lid.
7. After 30 minutes, check the temperature of the external sensor on the display or in the Incu-
Control software. If the sample temperature is still too low, raise the set values for the Heated
Plate and Heated Lid for 0.5–1°C, each, and wait until the sample temperature is stable again
(at least 10–15 min).
8. When the sample temperature has reached the desired value, write down the set values for
the Heated Plate and Heated Lid and use those settings for all upcoming experiments with the
same sample holder.
NOTE
– The temperature of the Heated Lid must be set to at least some degrees warmer
than the temperature of the Heated Plate!
When changing the incubation temperature, the Heated Glass Bottom must always be set to 1°C
lower than the temperature of the Heated Plate, e.g. if the Heated Plate is set to 39°C, the Heated
Glass Bottom must be set to 38°C. If the Heated Glass Bottom is not set 1°C lower, the temperature
distribution in the sample may not achieve the specified values.
The temperature adjustment must be repeated from time to time (at least once a year), especially if
one of the following conditions has been changed:
Version 1.0 (August 1, 2022)
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Instruction Manual
