ibidi Heating System, Multi-Well Plates, K-Frame
Table 1 – (continued)
Weight: 1720 g/3.8 lbs
Heated Lid
25 mm × 160 mm × 108 mm
Length of cable: 1.5 m
Connector to Gas Incubation: Female Luer Lock
Heated Plate
9 mm × 160 mm × 130 mm
K-Frame fitting: 160 mm × 110 mm
Observation area: 107.5 mm × 72.5 mm
Length of cable: 1.5 m
Heated Plate assembled with Heated Lid
Height: 30 mm
Weight: 430 g/0.95 lbs
USB cable
Length: 1.8 m
Power supply cable
Length: 2.0 m (power supply to wall)
Length: 1.2 m (power supply to device)
Temperature Control Range
Heated Lid (Channel 1)
Ambient temperature (min. 18°C) to +45°C
Heated Plate (Channel 2)
Ambient temperature (min. 18°C) to +45°C
Heated Glass Bottom (Channel 3)
Ambient temperature (min. 18°C) to +45°C
Temperature deviation
max. 2°C between wells of a Well-Plate
Recommended Temperatures before Adjustment
See Section
Optical Properties Heated Lid and Heated Glass Bottom
Glass
Selected HQ Floatglass
Thickness of the glass plate
1.1 mm/0.7 mm
Refractive index glass
n
D
= 1.520 (588 nm)
Thickness of ITO coating
100 nm
Passivation layer
Thickness: 20-25 nm
Refractive index ITO with passiv. layer
1.95
Requirements for Objective Lenses
Minimum 1 mm working distance for coverslip bottom plates, e.g., ibidi µ-Plate 96 Well
Minimum 2 mm working distance for thick bottom plates
The sample focal plane in the K-Frame lies up to 3.5 mm above the normal sample focal
plane. Therefore the objective must have at least 3.5 mm of travel distance along the focal
axis to be able to image the sample.
Microscope Requirements
Working distance condenser
≥
26 mm
Stage opening
160 mm × 110 mm
Instruction Manual
7
Version 2.0 (March 22, 2019)