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ibidi Heating System, Multi-Well Plates, K-Frame

4

Equipment

The ibidi Heating System, Multi-Well Plates, K-Frame consists of the Heated Lid, Heated Plate with
Heated Glass Bottom, and a Temperature Controller.

This section gives an overview of the components and their functionality and characteristics.

4.1

Components of the ibidi Heating System, Multi-Well Plates, K-Frame

The components of the ibidi Heating System, Multi-Well Plates, K-Frame (#10929) are listed below.

Description

Drawing

Temperature Controller

Heated Lid with electrical cable and D-sub connector to
connect to the Temperature Controller

Heated Plate with Heated Glass Bottom with electrical ca-
ble and D-sub connector to connect to the Temperature
Controller

Temperature Adjustment Set:
1 temperature sensor
1 perforated ibidi µ–Plate 24 Well

USB cable to connect the Temperature Controller with a
computer
Country specific power cord to connect the external power
supply to the wall socket

External power supply for the Temperature Controller

USB flash drive with IncuControl software and instruction
manual

Version 2.0 (March 22, 2019)

14

Instruction Manual

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Summary of Contents for 10929

Page 1: ... I 42 1 S 42 0 Run Plate Glass 38 0 38 0 36 9 37 0 Setup N C Version 2 0 Instruction Manual ibidi Heating System Multi Well Plates K Frame www ibidi com 10929 ibidi Heating System Multi Well Plates K Frame ...

Page 2: ...ulti Well Plates K Frame Contact ibidi GmbH Lochhamer Schlag 11 82166 Gra felfing Germany Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E mail info ibidi de Internet ibidi com Version 2 0 March 22 2019 2 Instruction Manual ...

Page 3: ...ng System Multi Well Plates K Frame 10 1 10 Repairing the ibidi Heating System Multi Well Plates K Frame 11 1 11 Waste Disposal WEEE RoHS Compliance Statement 11 1 11 1 EU Directive WEEE 11 1 11 2 EU Directive RoHS 12 2 Intended Use 13 3 Principle 13 4 Equipment 14 4 1 Components of the ibidi Heating System Multi Well Plates K Frame 14 4 2 Combination Options 15 4 3 Temperature Controller 16 4 4 H...

Page 4: ...p Mode 21 5 3 2 Incubation Parameters 21 5 3 3 Setup Menu 21 5 4 Temperature Adjustment in the Sample 23 5 5 Sample Preparation 24 6 IncuControl Software 25 7 Maintenance 26 7 1 Disinfection and Cleaning 26 7 2 Influence of Ambient Temperature and Ventilation 26 8 Troubleshooting 27 8 1 Focus not Stable 27 8 2 Evaporation Is too High 27 8 3 Condensation Inside the Stage Top Incubator 27 Version 2 ...

Page 5: ...al words WARNING CAUTION and NOTE have specific meanings in this man ual Do not proceed beyond a signal word until you have performed the indicated actions WARNING A potentially hazardous situation which if not avoided could result in se rious injury or even death Warning messages in the text are displayed in a gray shaded box CAUTION A potentially hazardous situation which if not avoided could re...

Page 6: ...age to channel 1 Heated Lid K Frame DC 10 V max 5 A Output voltage to channel 2 Heated Plate K Frame DC 24 V max 5 A Output voltage to channel 3 Heated Glass Bottom K Frame DC 10 V max 5 A Operating Conditions Operating site Indoor use only Operating temperature 18 30 C 64 86 F min 5 C 9 F less than set tempera ture Humidity 80 relative humidity RH up to 31 C 30 relative humidity RH up to 40 C Ope...

Page 7: ...ature deviation max 2 C between wells of a Well Plate Recommended Temperatures before Adjustment See Section 5 4 Optical Properties Heated Lid and Heated Glass Bottom Glass Selected HQ Floatglass Thickness of the glass plate 1 1 mm 0 7 mm Refractive index glass nD 1 520 588 nm Thickness of ITO coating 100 nm Passivation layer Thickness 20 25 nm Refractive index ITO with passiv layer 1 95 Requireme...

Page 8: ...a ground earth contact Do not replace detachable power cables by power cables with inadequate specifications By violating these instructions you risk electric shock and fire Only use extension cables that have a protective ground wire Do not operate the ibidi Heating System Multi Well Plates K Frame under conditions that pose a risk of explosion implosion or the release of gases Only operate the i...

Page 9: ... Install the ibidi Heating System Multi Well Plates K Frame in a location that enables easy access for maintenance Do not place heavy objects on the instrument The heated glass plates of the incubation chamber can break on mechanical impact If so the glass shards can lead to injuries if handled Be aware that when switched on a 10 V DC voltage is applied to the underside of the glass on the Heated ...

Page 10: ...rrounding gases 7 Accidents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tubes fluidic connectors reagents etc The warranty for all parts supplied and repairs provided under this warranty expires on the warranty expiration date of the original product 1 9 Transporting the ibidi Heating System Multi Well Plates ...

Page 11: ...ng the use of certain substances Restric tion on the use of Hazardous Substances RoHS 1 11 1 EU Directive WEEE The ibidi Heating System Multi Well Plates K Frame must be disposed of in compliance with the WEEE Directive 2012 19 EC This symbol on the product is in accordance with the European Union s Waste Electrical and Elec tronic Equipment WEEE Directive The symbol indicates that this product mu...

Page 12: ...l devices with the exception of implanted or infected products and Category 9 monitoring and control instruments All of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive Nevertheless the ibidi Heating System Multi Well Plates K Frame meets the requirements set forth in the RoHS Directive 2011 65 EC Version 2 0 March 22 2019 12 Instruction Manual ...

Page 13: ...s as well as the humidity 3 Principle The Heated Lid and Heated Plate with the Heated Glass Bottom are designed to keep cells on stage in a multiwell plate at 37 C The Heated Plate is mounted on the microscope stage The multiwell plate is placed directly on the Heated Glass Bottom and the Heated Lid is put on top The gas mixture is streamed into the chamber through the gas inlet and then slowly es...

Page 14: ...w Description Drawing Temperature Controller Heated Lid with electrical cable and D sub connector to connect to the Temperature Controller Heated Plate with Heated Glass Bottom with electrical ca ble and D sub connector to connect to the Temperature Controller Temperature Adjustment Set 1 temperature sensor 1 perforated ibidi µ Plate 24 Well USB cable to connect the Temperature Controller with a c...

Page 15: ...ll plates matching the ANSI SLAS 1 2004 standard R2012 with a maximum height of 23 mm The ibidi Heating System Multi Well Plates K Frame can be combined with the Gas Incubation System that provides CO2 and O2 optional control and a defined humidified atmosphere Detailed information on the Gas mixer unit is given in the Gas Incubation System instructions Figure 3 Overview of the parts of the ibidi ...

Page 16: ...erature Controller via PC use the IncuControl software Section 6 Figure 4 Front view of the Temperature Controller The LEDs on the front indicate the status of the channels connection to the power supply and USB connection Figure 4 Control LEDs Channel 1 4 LED off Channel inactive LED on Channel active LED fast blinking Channel error All plugs for the electrical connections are integrated into the...

Page 17: ...re Controller 4 4 Heated Lid The Heated Lid provides excellent optical quality and also allows for the use of all standard mi croscopy techniques including differential interference contrast DIC Due to the height of the lid we recommend using condensers with a working distance of 26 mm The upper glass part of the Heated Lid is electrically heat controlled The Heated Lid fits exactly and securely o...

Page 18: ... the Heated Plate fits into a mechanical or scanning stage with opening for mounting frames K 160 mm 110 mm Figure 7 Heated Plate top view The Heated Plate can be leveled to compensate uneven samples Use the 0 9 mm INBUS Hex Key to adjust the M2 set screws at each of the plate s corners Figure 8 Leveling of the Heated Plate Version 2 0 March 22 2019 18 Instruction Manual ...

Page 19: ...te The resulting temperature in the sample must be adjusted for your specific setup Follow the instructions in Section 5 4 5 1 Installation and Connection of the Parts The components of the ibidi Heating System Multi Well Plates K Frame are connected as shown in Figure 9 Figure 9 Installation of the components of the ibidi Heating System Multi Well Plates K Frame 1 Place the Temperature Controller...

Page 20: ...0V DC voltage is applied to the underside of the glass plates Do not touch the underside or contact it with anything conductive This could cause a short circuit that may destroy the controller and or the lid If you are not sure which temperature is set e g when operating the system the first time it is recommended to disconnect all heated devices Heated Plate Heated Lid and other optional heated d...

Page 21: ...Plate Universal Fit for 1 Chamber 10918 or Heated Plate Multi Well Plates K Frame 10929 Channel 3 Glass Heated Glass Bottom Multi Well Plates K Frame 10929 Channel 4 Ch4 Heated Plate Universal Fit for 4 µ Slides 10927 10928 5 3 1 Run Stop Mode Set the whole system to run or stop mode by manipulating the setting in the display s bottom line to Run system is running or Stop system is not running Fig...

Page 22: ...mode menu to the alarm menu by pressing the right button The maximum and minimum limits of the alarm can be set for each individual channel If the alarm is activated which happens when the current value goes under over the low high limits the current value I blinks showing alternately the value and low or high respectively To stop the display blinking navigate to the blinking channel and press the...

Page 23: ...mple temperature use the provided Temperature Adjustment Set The temperature of the heated components should be adjusted in small steps Follow this procedure to adjust the sample temperature 1 Connect the temperature sensor thermocouple type K to the plug labeled Temp Sensor on the Temperature Controller s back 2 Fill the provided multi well plate with water The volume of water and type of plate s...

Page 24: ...t once a year especially if one of the following conditions has been changed Room temperature Air conditioning Chamber type or objective lens Humidification and gas flow Use of an XL Incubator Use of an Objective Heater 5 5 Sample Preparation Prepare the cells according to your protocol and place the multi well plate in the Heated Plate mounted on the microscope stage Close the Heated Lid Let the ...

Page 25: ... can be downloaded from the ibidi website For more details please refer to the IncuControl instructions Figure 16 Temperature control window in IncuControl Prohibitions on the use of ibidi software Copying software for other than backup purposes Transfering or licensing of the right to use software to a third party Disclosure of confidential information regarding software Modification of software ...

Page 26: ...c solvents may remove the instrument s paint To clean the heated components and or the Temperature Controller switch off the Temperature Con troller and disconnect all electrical cables Leave the instrument to cool down for approx 5 minutes WARNING Hot surface max 55 C Do not touch Heated Lid Heated Plate and Heated Glass Bottom when hot 7 2 Influence of Ambient Temperature and Ventilation The amb...

Page 27: ...uggest using silicone oil e g Anti Evaporation Oil ibidi 50051 to decrease evaporation Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equilibrate oil and medium inside the incubator overnight This step helps to avoid the formation of air bubbles and pre warms...

Page 28: ...82166 Gräfelfing München Germany Toll free within Germany Phone 0800 00 11 11 28 Fax 0800 00 11 11 29 International calls Phone 49 89 520 46 17 0 Fax 49 89 520 46 17 59 E Mail info ibidi com www ibidi com Certified ISO 9001 2008 EN ISO 13485 2012 ...

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