ibidi Pump System
Figure 52: Example of a calibration curve showing the relation between flow rate and pressure.
4. This calibration curve enables the prediction of the pressure needed to generate the desired
flow rate (p in mbar,
Φ
in ml/min).
p
=
Φ
0.71
=
13.3
0.71
=
18.7
5. In the PumpControl Software, choose the Perfusion Set being used from the drop down menu
and choose the option “without any slide” from the slide drop down menu. Apply the pressure
of 18.7 mbar to the Fluidic Unit and ignore the flow rate value that is indicated in the software.
Figure 53: Selection of Perfusion set red and Slide selection “without any slide”
Figure 54: Inserting 18.7 mbar in the pressure box. The flow rate is
13.3 ml/min, as indicated in the calibration curve. The automatically
calculated flow rate must be ignored.
Always check the flow rate when running an experiment. Because of small manufacture toler-
ances of the slides and tubing, the values vary slightly. Section
describes how to calibrate
the system.
6. The PumpControl Software does not know the correct flow rate, and therefore, the program
is not able to calculate the right switching time. Insert the switching time manually to avoid
having the reservoirs run dry.
Instruction Manual
69
Version 2.6 (May 12, 2022)
