ibidi Pump System
Figure 28: Fill the syringe reservoirs with medium to equal levels and connect the filters to
the reservoirs again.
6.4
Remove Air Bubbles from the Perfusion Set
Once the system is set up and the Perfusion Set contains medium, air bubbles remaining in the
branched tubing arms must be removed. To protect the cells from being flushed out, it is critical
to remove all air bubbles from the Perfusion Set before connecting it to a slide containing cells.
To remove air bubbles, start the pump with the PumpControl software. Refer to the
for detailed instructions.
1. Equilibrate the liquid levels of the two reservoirs using the manual control panel in the Pump-
Control software.
2. Set an automatic cycle with a high pressure (50–80 mbar) and let the cycle run for at least 5
minutes. Then check the flow tubing to confirm that all the air bubbles have been removed.
Handling Tip!
If working with one Perfusion Set connected to port 1 of the pump, you can load a pre-installed
routine in the software “Remove air bubbles” (Tutorial
→
Load demo setups
→
Remove air bub-
bles) and let it run for at least 5 minutes.
Version 2.6 (May 12, 2022)
42
Instruction Manual
