E9Screen Software
EPC9 Manual
47
Sound Settings
: Sensitivity (Hz/M
Ω
) and volume
(in %) of the sound encoding of R-membrane can be
specified here. To enable the sound option press the
Sound
button.
CC Fast Speed
: Clicking on this button enables the fast current-
clamp mode (only version “C” and later versions of the EPC9).
For details see
Chapter
6. Operating Modes
on page 54
).
Gentle CC-Switch
: This control selects between the
two alternate modes of switching between voltage
and current clamp and back.
When selecting
Gentle CC-Switch:
On
,
I-hold
will be set to inject the holding current
required to keep the membrane voltage constant while switching to current clamp
mode. Thus, V-mon will be identical to the original
V-membrane
commanded under
voltage clamp. In analogy, upon returning to voltage clamp,
V-membrane
will be set
according to the membrane potential under current clamp and rounded to the next 2
mV.
When selecting
Gentle CC-Switch:
Off
,
I-hold
will be set to 0 pA when switching to
current clamp mode.
Last V-membrane
: Quite often, the membrane potential under
current clamp will have changed, and when returning to voltage
clamp,
V-membrane
will possibly differ from that set before switching to current-
clamp mode.
Last V-membrane
can then be used to conveniently restore the original
V-membrane
value.
CC-range
: Two current clamp scaling ranges can be
selected: 1 and 10 pA/mV (with the exception of the
EPC9 version “A” and “C”, which have a fixed CC-scaling of 1 pA/mV). When the
CC-scaling is set to 1 pA/mV, a command pulse of 1 mV gives rise to 1 pA in
Current
Clamp
mode. This relationship of 1 and 10 pA/mV holds regardless of the
Gain
setting. The maximal output current in
Current Clamp
mode is 1 and 10 nA,
respectively.
Relative Value
: This button is useful when recording
macros. A change of any value will be recorded as a
relative change, e.g. if you hyperpolarize the holding potential from -60 to -70 mV
while recording a macro, a step of -10 mV will be recorded (see description of Macros
at the beginning of the subchapter Main Controls).
Sound
Volume
Hz/M
Ω
Rmem
100 %
100
Gentle CC-Switch: ON
CC-range: 1pA/mV
Relative Value
