HEKA EPC 9, Manual

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Table of Contents EPC9 Manual 4
Coating ............................................................................................................................ 72
Heat Polishing ................................................................................................................... 73
Use of Pipettes .................................................................................................................. 73
10. Using the Patch Clamp 75
Forming a Seal _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 75
Initial Setup ....................................................................................................................... 75
Entering the Bath ............................................................................................................... 75
Forming a Gigaseal ............................................................................................................ 76
Cell-Attached Recording _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 76
Whole-Cell Recording _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 77
Breaking the Patch ............................................................................................................. 77
Capacitive Transient Cancellation .......................................................................................... 78
Series Resistance Compensation .......................................................................................... 78
Current-Clamp Recording_ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 79
11. Low-Noise Recording 80
Appendix I: Controlling E9SCREEN 83
Communication between E9Screen and other Programs _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 83
Reserving the AD/DA-board for exclusive use .......................................................................... 83
The “EPC9out.EPC” file ....................................................................................................... 83
Controlling the EPC9 from another Program _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 85
Sending Commands to E9Screen .......................................................................................... 85
Error Messages.................................................................................................................. 87
Implemented Commands and Messages................................................................................. 87
Notes to Programmers......................................................................................................... 91
Sample program ................................................................................................................ 93
Appendix II: Technical Data 98
Digital I/O Connector _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ _ 98
Standard EPC9.................................................................................................................. 98
EPC9 Double and Triple ...................................................................................................... 99
Index 101