Harvard Bioscience 800 2770 User Manual Download

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CMA 470 REFRIGERATED FRACTION COLLECTOR USER’S MANUAL

CONTENTS

INTRODUCTION

.................................................................................................... 3

SAFETY

................................................................................................................... 3

UNPACKING & ASSEMBLY

............................................................................... 4

3.1

Packing list

...................................................................................................... 4

3.2

Positioning the Unit

........................................................................................ 4

3.3

Connections

.................................................................................................... 4

3.4

Installation of accessories

............................................................................. 4

DESCRIPTION

....................................................................................................... 5

4.1

Control Panel

................................................................................................... 5

4.2

Rear

.................................................................................................................. 6

4.3

Power Switch

................................................................................................... 6

4.4

Cassette Magazine

.......................................................................................... 7

4.5

Vial cassettes

.................................................................................................. 7

4.6

Cannula for Tubing

......................................................................................... 9

4.7

Cannula for Septa

........................................................................................... 9

4.8

Single collect Assembly

............................................................................... 10

4.9

Dual – Quadruple Collect Assembly

........................................................... 11

4.10

Basic Functions

............................................................................................ 12

4.11

Safety Devices

............................................................................................... 12

OPERATING INSTRUCTIONS

.......................................................................... 13

5.1

POWER on

..................................................................................................... 13

5.2

Set collection mode

...................................................................................... 13

5.3

Set temperature

............................................................................................. 14

5.4

Set first sample and number of samples

................................................... 14

5.5

Set fraction time

............................................................................................ 15

5.6

Prepare for collection experiment

.............................................................. 15

5.7

Connect the sample set up

.......................................................................... 15

5.8

Start/Stop

....................................................................................................... 15

5.9

Save settings

................................................................................................. 16

5.10

Reset settings

............................................................................................... 16

5.11

Clean the system after use

.......................................................................... 16

ADVANCED OPERATION

................................................................................. 16

ACCESSORY KIT

................................................................................................ 18

OPTIONAL ACCESSORIES

.............................................................................. 18

8.1

Vials

................................................................................................................ 18

8.2

Cassettes for collection into 2 mL vials

..................................................... 18

8.3

Cables

............................................................................................................ 18

MAINTENANCE & SERVICE

............................................................................ 18

9.1

Cleaning the Instrument

............................................................................... 18

9.2

Storage

........................................................................................................... 18

9.3

Warranty

......................................................................................................... 19

9.4

Damaged shipments

..................................................................................... 19

9.5

Service

........................................................................................................... 19

TROUBLESHOOTING

.................................................................................... 20

TECHNICAL DATA

......................................................................................... 20

TEXT AND SYMBOL EXPLANATION

........................................................ 21

ORDER INFORMATION

.................................................................................... 21

8002778 Rev 1.0

Summary of Contents for 800 2770

Page 1: ...8002778 Rev 1 0 CMA 470 Refrigerated Fraction Collector ...

Page 2: ...TRUCTIONS 13 5 1 POWER on 13 5 2 Set collection mode 13 5 3 Set temperature 14 5 4 Set first sample and number of samples 14 5 5 Set fraction time 15 5 6 Prepare for collection experiment 15 5 7 Connect the sample set up 15 5 8 Start Stop 15 5 9 Save settings 16 5 10 Reset settings 16 5 11 Clean the system after use 16 6 ADVANCED OPERATION 16 7 ACCESSORY KIT 18 8 OPTIONAL ACCESSORIES 18 8 1 Vials ...

Page 3: ...2 ...

Page 4: ... collect samples in open vials 2 SAFETY CMA 470 Refrigerated Fraction Collector is designed for laboratory use only e g animal experiments analytical chemistry etc and is therefore not equipped with the special safety functions that are necessary for use in studies involving humans In view of this the following considerations should always apply The CMA 470 Refrigerated Fraction Collector should o...

Page 5: ...t check the contents against the above packing list to ensure that the shipment is complete Inspect all items for damage Any damage or missing parts should be reported immediately to your local supplier or CMA Microdialysis AB Remove the transport security material 3 2 Positioning the Unit The CMA 470 Refrigerated Fraction Collector can stand directly on a laboratory workbench or any other flat st...

Page 6: ...socket the instrument is ready for use 4 1 Control Panel 1 2 3 4 5 6 7 8 Figure 4 1 Control panel Locate the following items on the control panel of the CMA 470 Refrigerated Fraction Collector See figure 4 1 Item Description 1 Display window Indicates all basic settings and status Status Mode Current samples fraction time total time and temperature Cooling activity 2 Menu Advanced settings 3 Reset...

Page 7: ...port 2 RS232 Socket for RS232 interface for connection to PC or other device with serial port 3 Digital I O Socket for digital Input Output for external equipment such as CMA 400 or other instruments 4 Power Socket for 12 VDC inlet Connection for power supply 4 3 Power Switch 1 Figure 4 3 Power Switch Power Switch on the right side of the panel Item Description 1 Power Switch Switch the power on T...

Page 8: ...mbering of cassettes 5 Supporting arm for cannula holder To mount the holder for single or quadruple cannulae on 6 Cannula for Septa For use when collecting in vials with Teflon septa 7 Holder for Single Cannula For use in single collecting mode with cannula for tubing or for septa 4 5 Vial cassettes Two options are available 64 x 300 µl vials H 35 OD 5 mm or 40 x 2 ml vials H 35 OD 11 mm All four...

Page 9: ...ple cannulae NOTE When three cannulae are in use numbering is as for four cannulae Every fourth position will be empty Step sequences for large vials Figure 4 5 2 Numbering of the cassette positions for single and dual cannulae NOTE When collecting into large vials up to 2 cannulae can be used simultaneously ...

Page 10: ...6 Cannula for Tubing To collect from tubing into the bottom of an open vial use this option that consists of a cannula and a plastic knob The tubing from the probe is fed through the supporting cannula until it touches the bottom of the collecting vial Secure the tubing in place by lifting the plastic knob NOTE Make sure the tubing is not pushed so far into the vial that the end bends 4 7 Cannula ...

Page 11: ...n it is mounted in the Multi Holder for Cannula Secure the cannula with the screw in the Multi Holder for Cannula 8 See Figure 4 8 2 4 8 3 Collecting in vials with septa Place a waste vial in position 1 in the cassette and move it to the collection position Insert the Cannula for Septa 9 in the Multi Holder for Cannula 6 Adjust the lower end of the cannula into the bottom of the vial 10 Secure the...

Page 12: ... 4 9 3 Collecting in vials with septa Place waste vials in the cassette and move them to the collection position Insert the Cannulae for Septa 7 in the Multi Holders for Cannula 2 Adjust the lower ends of the cannulae into the bottom of the vials 8 Secure the cannulae with the screws in the Multi Holders for Cannula 6 See Figure 4 9 3 4 9 4 To remove the Holder for Dual or Quadruple Cannulae untig...

Page 13: ...the vials In this way a very high degree of precision is achieved and fractions smaller than a falling droplet can be collected in a reproducible manner When fraction are collected in vials fitted with a septum some of the liquid may be lost against the septum upon withdrawal of the piercing needle such that the quantity collected in each vial may vary depending on the rate of flow Change of fract...

Page 14: ...trument for the experiment cassettes and cannulae See 4 5 4 9 for setting up the system 5 1 POWER on Connect to the external power supply Switch POWER on The display illuminates Fig 4 1 4 3 5 2 Set collection mode Available Modes Single Dual and Quadruple mode NOTE Use Quadruple mode for triple cannula collection Turn the Selection Knob to move the cursor to the upper row Press the Selection Knob ...

Page 15: ...Selection Knob to select cooling on off Press the Selection Knob to acknowledge and lock the setting Figure 5 3 Setting temperature 5 4 Set first sample and number of samples Sample collection starts one position after the current position waste position in the cassette magazine NOTE First collecting fraction will be the position after the cannula location at the start Turn the Selection Knob to m...

Page 16: ... Setting fraction time 5 6 Prepare for collection experiment Load the cassettes with collection vials Position waste vials at the first position Press Cassette and Step buttons to position the waste vials at cannula e position See Figures 4 5 1 and 4 5 2 for numbering of vials 5 7 Connect the sample set up When collecting straight from the tubing into the bottom of the collection vial feed the tub...

Page 17: ...up the CMA 470 for a new sample method single dual quadruple mode or for the set up of collection for tubing or cannula manual commands is used to control sampling sequences When the Manual function is activated cassettes can be moved Up or Down and the Multi Holder can be moved from the Outer to Inner vial row and back Single mode with commands from the Menu Press the front panel buttons Cassette...

Page 18: ...17 Figure 6 1 Menu options Figure 6 2 Menu Manual operations The menu functions Set Up and Info are only for maintenance and service purpose Information can be found in the technical manual ...

Page 19: ...be used Large vial cassettes must be placed in all four positions in the magazine A maximum of two collection cannulae can be used with this option 8 3 Cables CMA RS232 PC RJ45 Cable for computer control of CMA 470 USB cable type A B for computer control of CMA 470 9 MAINTENANCE SERVICE The CMA 470 Refrigerated Fraction Collector does not require periodic lubrication or changes of any parts 9 1 Cl...

Page 20: ... whatsoever The foregoing warranty is in lieu of all other warranties of merchantability and fitness for a particular purpose 9 4 Damaged shipments Breakages of any part of this instrument during shipping should be reported immediately to CMA Customer Service or an authorized representative You must retain the original packing box and contents for inspection by the freight handler CMA will replace...

Page 21: ...es and seconds Environment temperature 10 32 C Display LCD 4 x 20 characters back lit Memory Non volatile Stores all settings External connections General purpose digital Input Output Computer connection RS 232 serial interface and USB for user software Voltage 100 240 VAC 50 60 Hz Power consumption External adaptor 12 VDC 55 W Dimensions 222 x 279 x 142 167 mm W x D x H Weight Approx 3 8 kg Stand...

Page 22: ...r Quadruple Cannulae Cannula for Tubing 4 pcs Cannula for Septa 4 pcs Accessories Vials Plastic 300 µL 1000 pkg 743 1100 Caps Plastic 1000 pkg 743 1101 Vials Glass 300 µL 500 pkg 743 1007 Caps Seals Non Reclosing Small 1000 pkg 743 2175 Vials Glass 2 mL 500 pkg 743 2009 Caps Seals Reclose Large 500 pkg 743 2011 Crimper Small 743 2017 Crimper Large 743 2018 Cassette Small Vials Plastic 832 0010 Cas...

Page 23: ...E 164 40 Kista Sweden Tel 46 8 470 10 00 Fax 46 8 470 10 50 E mail support hbiosci com www microdialysis com Harvard Apparatus 84 October Hill Road Holliston MA 01746 USA Phone Orders 800 232 2380 Fax 508 429 5732 E mail support hbiosci com www harvardapparatus com ...

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