Publication 5419-013 REV1.0
4
Product Overview- Theory of Operation
The Harvard Apparatus Pump 33 DDS (Dual Drive System)
is a leap forward in syringe pump capability. The Pump 33
DDS has two independent pumping channels controlled by
an intuitive touch screen interface.
This multi-purpose syringe pump employs advanced syringe
mechanisms that include a tight gripping, extremely secure
syringe clamp that accommodates syringe sizes 0.5 µl to 60
ml. The Pump 33 DDS offers enhanced flow performance
with high accuracy and smooth flow from 1.02 pl/min to
106 ml/min, syringe size dependent.
The Pump 33 DDS uses a 7” LCD color touchscreen interface
and advanced application software to control operation.
The display run screen presents the user with all key
dispensing parameters in real time.
The application software provides convenient selection of
common syringe models from a syringe Lookup Table, as
well as insertion of custom syringe specifications. Syringe
tables containing all major syringe manufacturers allow
simple selection of any compatible syringe size. The system
is then able to calculate the cross-sectional area of the
syringe selected and calibrate the flow rate and volume
accumulation, displaying real-time operating characteristics
via on-screen graphics.
I/O interfaces permit external control via an independent
computer or device.
Features
Three operating conditions named Independent,
Reciprocating and Twin are available to accommodate
a wide range of setups and experimental protocols.
Independent Condition
Independent Condition allows the Pump 33 DDS to operate
as two separate syringe pumps named P1 & P2. P1 is
syringe position 1, closest to the touch screen interface
and P2 is syringe position 2 and is toward the backside
of the unit.
Each syringe will operate independently with different
syringe types, size, force, target (volume or time) and flow
rate settings. This innovative condition allows you to run
two different flows at the same time using one instrument.
Reciprocating Condition
In Reciprocating Condition, the syringe channels move
in opposite directions at the same rate using the same
syringe size and type. When combined with a valve box, the
reciprocating condition can provide the continuous fluidic
delivery of a peristaltic pump with the accurate, pulseless,
low flow rates provided by a syringe pump.
Twin Condition
The Twin Condition allows both syringes to operate in the
same mode using the exact same syringe type, syringe size,
force, target (volume or time) and flow rate settings.
The pump also allows the user to combine both flows using
the Ganging feature for higher speed and larger volume
infusion applications.
Advanced Connectivity
The Pump 33 DDS comes standard with USB and RS-232
connections for PC communication and RS-485 for pump-to-
pump communication. An entire suite of ASCII commands
is available to control the pump remotely with a PC. The
pump contains a footswitch input and digital input/output
for each independent pumping channel.
Adjustable Motor Force
The Pump 33 DDS motors generate up to 70 lbs of linear
force in operation. This force is sufficient to damage
delicate, low-volume syringes, as well as many standard
syringes if pumping viscous fluids. The application software
provides controls to allow you to set a maximum force value
(calculated as a percentage of the maximum value).
Independent Condition Functional Table (Mode Dependent)
Reciprocating Condition Functional Table (Mode Dependent)
Twin Condition Functional Table (Mode Dependent)
Mode
Syringe
Rate
Target
Volume/TIme
P1
Infuse, Withdraw,
Infuse/Withdraw,
Withdraw/Infuse
Any size/type
0.5 µl - 60 ml
Any within
syringe
capability
Any (mode
dependent)
P2
Infuse, Withdraw,
Infuse/Withdraw,
Withdraw/Infuse
Any size/type
0.5 µl - 60 ml
Any within
syringe
capability
Same as P1
Mode
Syringe
Rate
Target
Volume/TIme
P1 Infuse/Withdraw,
Withdraw/Infuse
Any size/type
0.5 µl - 60 ml
Any within
syringe
capability
Any
P2
Opposite of P1
Same as P1
Same as P1
Same as P1
Mode
Syringe
Rate
Target
Volume/TIme
P1
Infuse, Withdraw,
Infuse/Withdraw,
Withdraw/Infuse
Any size/type
0.5 µl - 60 ml
Any within
syringe
capability
Any (mode
dependent)
P2
Same as P1
Same as P1
Same as P1
Same as P1
