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Measurement Procedure

• Press the ON/OFF button to turn the checker 

on. All segments will be displayed for a few 

seconds, followed by “Add”

“C.1” with “Press” 

blinking.

• Using the 10 mL syringe, measure exactly 7 mL 

of sample into the large mixing vial.

• Using the 5 mL syringe, add exactly 4 mL of 

HI781A-0

 reagent into the large mixing vial.

• Add the content of one packet of 

HI781B-0

 

reagent into the large mixing vial. Replace the 

cap and shake vigorously for 1 minute.

• Remove the plunger from the 10 mL syringe. 

Attach the syringe to the filter holder assembly 

using the threaded connection.

• Remove the cap from the mixing vial. Hold 

the syringe and filter holder assembly over 

a cuvette. Carefully pour the contents of the 

mixing vial into the back of the syringe.

• Very slowly push the plunger into the 10 mL 

syringe until the 10 mL cuvette has been filled 

up to the 10 mL mark and replace the cap. 

Insert the cuvette into the checker and close 

the cap.

• Press the ON/OFF button. When the display 

shows “Add”

“C.2” with “Press” blinking, the 

checker is zeroed.

• Remove the cuvette, unscrew the cap and 

add the content of one packet of 

HI781C-0

 

reagent. Replace the cap and shake vigorously 

for 2 minutes.

• Insert the cuvette into the checker.

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Dear Customer, 

Thank you for choosing a Hanna Instruments product. Please read this 
instruction manual carefully before using the Checker

®

HC handheld 

colorimeter. For more information about Hanna Instruments and our 
products, visit www.hannainst.com or e-mail us at [email protected].
For technical support, contact your local Hanna Instruments Office or e-mail 
us at [email protected].

Preliminary Examination

Remove the Checker

®

HC handheld colorimeter and accessories from the packing 

material and examine it carefully. If you require any further information, please 

contact Hanna Instruments technical support team.

Each 

HI781

 is delivered in a case with custom insert and is supplied with:

• Sample cuvette and cap (2 pcs.)

• Marine Nitrate Low Range reagent starter kit (reagents for 25 tests)

• Filter paper (25 pcs.)

• Filter holder (1 pc.)

• Mixing vial and cap (1 pc.)

• 5 mL syringe with tip (1 pc.)

• 10 mL syringe (1 pc.)

• 1.5V AAA Alkaline battery (1 pc.)

• Instruction manual

Note: 

Save all packing material until you are sure that the Checker

®

HC 

handheld colorimeter works correctly. Any damaged or defective item must 

be returned in its original packing material with the supplied accessories.

General Description & Intended Use

HI781

 Marine Nitrate Low Range handheld checker is designed to accurately 

determine nitrate levels in aquariums and marine biology applications. 

HI781

 features a single-button operation system and is easy to use.

The large LCD is easy to read and the auto shut-off feature assures the battery 

life will not be drained.

Specifications

Range

0.00 to 5.00 ppm (as NO

3

)

Resolution

0.01 ppm

Accuracy

±0.25 ppm ±2% of reading @ 25 °C (77 °F)

Light source

Light Emitting Diode @ 525 nm

Light detector

Silicon photocell

Method

Colorimetric method. The reaction between nitrate and the 

reagent causes a pink/violet tint in the sample. This meter 

has been developed to work with seawater samples.

Environment

0 to 50 °C (32 to 122 °F); max. 95% RH non-condensing

Battery type

1.5V AAA Alkaline

Auto shut-off

After 20 minutes of non-use and 4 minutes after reading

Dimensions

86.0 x 61.0 x 37.5 mm (3.4 x 2.4 x 1.5”)

Weight

64 g (2.3 oz)

Functional Description & LCD Display

Cap
Cuvette with cap

Cuvette holder

LCD (Liquid Crystal Display)

ON/OFF button

Prepare the Filter Holder Assembly

Unscrew the two halves of the reusable filter holder and carefully place one paper 

filter on the lower piece (the lower piece has no threads on the outlet). Ensure the 

filter paper is on top of the clear colorless gasket in the filter holder.

Thread the upper piece over the lower piece and tighten securely. Ensure that the 

paper filter is not overlapping the threads.

The filter holder assembly is now ready for use.

• Press and hold the ON/OFF button. The display will 

show the countdown prior to the measurement. 

Alternatively, wait 8 minutes and press the button.

• When the timer ends the checker will perform 

the reading. The instrument displays the 

concentration of nitrate in ppm of NO

3

. The 

checker turns off 4 minutes after reading.

Errors & Warnings

The checker shows clear warning messages when erroneous conditions appear and 

when measured values are outside the expected range. The information below 

provides an explanation of the errors and warnings, and the recommended action 

to be taken.

Light High:

 There is an excess amount of ambient light 

reaching the detector. Please check the preparation of the zero 

cuvette.

Light Low:

 There is not enough light to perform a measurement. 

Please check the preparation of the zero cuvette.

Inverted Cuvettes:

 The sample and the zero cuvettes are 

inverted. Swap the cuvettes and repeat the measurement.

Under Range:

 A blinking “0.00”

 

indicates that the sample 

absorbs less light than the zero reference. Check the 

measurement procedure and make sure to use the same cuvette 

for reference (zero) and measurement.

Over Range:

 Maximum concentration value displayed blinking 

indicates the measured value is outside the limits of the method.

Verify that the sample does not contain any debris. Dilute the 

sample and repeat the measurement.

Battery Low:

 Battery level is too low for the checker to function 

properly. Replace the battery with a new one.

Drained Battery:

 The battery is drained and 

must be replaced. Replace the battery with a 

new one and restart the checker.

Summary of Contents for HI781

Page 1: ...HI781 Marine Nitrate Low Range INSTRUCTION MANUAL ...

Page 2: ...stom insert and is supplied with Sample cuvette and cap 2 pcs Marine Nitrate Low Range reagent starter kit reagents for 25 tests Filter paper 25 pcs Filter holder 1 pc Mixing vial and cap 1 pc 5 mL syringe with tip 1 pc 10 mL syringe 1 pc 1 5V AAA Alkaline battery 1 pc Instruction manual Note Save all packing material until you are sure that the Checker HC handheld colorimeter works correctly Any ...

Page 3: ...utes of non use and 4 minutes after reading Dimensions 86 0 x 61 0 x 37 5 mm 3 4 x 2 4 x 1 5 Weight 64 g 2 3 oz Functional Description LCD Display Cap Cuvette with cap Cuvette holder LCD Liquid Crystal Display ON OFF button Prepare the Filter Holder Assembly Unscrew the two halves of the reusable filter holder and carefully place one paper filter on the lower piece the lower piece has no threads o...

Page 4: ... assembly using the threaded connection Remove the cap from the mixing vial Hold the syringe and filter holder assembly over a cuvette Carefully pour the contents of the mixing vial into the back of the syringe Very slowly push the plunger into the 10 mL syringe until the 10 mL cuvette has been filled up to the 10 mL mark and replace the cap Insert the cuvette into the checker and close the cap Pr...

Page 5: ... zero cuvette LightLow Thereisnotenoughlighttoperformameasurement Please check the preparation of the zero cuvette Inverted Cuvettes The sample and the zero cuvettes are inverted Swap the cuvettes and repeat the measurement Under Range A blinking 0 00 indicates that the sample absorbs less light than the zero reference Check the measurement procedure and make sure to use the same cuvette for refer...

Page 6: ...will be affected Discard the sample immediately after the reading has been taken or the glass might become permanently stained Battery Replacement To save the battery the checker shuts down after 20 minutes of non use and 4 minutes after reading A fresh battery lasts for a minimum of 5000 measurements When the battery is drained the instrument displays bAd then bAt and turns off To replace the bat...

Page 7: ...yringe 10 pcs HI93703 50 Cuvette cleaning solution 230 mL Certification All Hanna Instruments conform to the CE European Directives Disposal of Electrical Electronic Equipment The product should not be treated as household waste Instead hand it over to the appropriate collection point for the recycling of electrical and electronic equipment which will conserve natural resources Disposal of waste b...

Page 8: ...ring or lack of prescribed maintenance is not covered If service is required contact your local Hanna Instruments Office If under warranty report the model number date of purchase serial number and the nature of the problem If the repair is not covered by the warranty you will be notified of the charges incurred If the checker is to be returned to Hanna Instruments first obtain a Returned Goods Au...

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