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• If the solution is clouded, you can improve separation between the chloroform and aqueous
layers by gently warming the cuvette (for instance by holding the cuvette in the hand).
• If the chloroform layer contains some aqueous drops hanging on the cuvette wall, gently swirl
or invert the cuvette.
• It is important to transfer at least 7 mL of chloroform layer into the measurement cuvette, thus
up to 0.5 cm (1/4”) below the 10 mL mark. If the transferred volume is lower than 7 mL, the
accuracy of the test may be affected. Please repeat the test waiting for longer than 2 minutes,
to allow complete separation between the two phases.
• Cap the cuvette. This is the reacted sample (
#2
).
• Fill another cuvette with 10 mL of Chloroform reagent up to the 10 mL mark and place the cap.
This is the blank (
#1
).
• Place the blank (cuvette
#1
) into the cuvette holder and ensure that the notch on the cap is
positioned securely into the groove.
MEASUREMENT PROCEDURE
#1