18
• Close tightly the glass vial with its cap and shake it vigorously for 30 seconds.
Note: Block the cap with a finger during shaking
.
• Press and hold
READ /TIMER
for three seconds and the display will show the countdown.
The beeper is playing a beep at the end of countdown period.
Alternatively, wait for 2 minutes leaving the vial capped and undisturbed
During this period, the chloroform layer separates from the aqueous layer.
• Remove the cap.
• Insert a clean plastic pipette below the upper aqueous layer to transfer only the lower chloroform
layer into a cuvette paying attention not to transfer the upper aqueous layer too.
• The solution in the cuvette must be limpid.
MEASUREMENT PROCEDURE
3’’