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Instructions for Use 

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CultureWell™ MultiWell Chambered Coverslips

 

 

11.

 

To mount coverslips on glass microscope slides, first remove the silicone gasket by peeling it off 
as shown in Figure 10. 

 

 

Figure 10 

 

Specimens may also be mounted using aqueous media by filling wells and pressing another 
coverslip onto the gasket. 

 

12.

 

To Clean and Re-Use MultiWell Gaskets 
 
Rinse off reagents immediately after each use.  Wash the gaskets with distilled water and if 
necessary with a mild detergent that is suitable for cell culture.  Do not soak gaskets in detergent.  
Rinse thoroughly with distilled water and allow gaskets to dry.  When re-using cleaned gaskets, 
just prior to applying them to a new coverslip, use a piece of adhesive tape to clean lint and 
debris from the gasket surface. 
 
Place a clean, dry 24mm x 50mm glass coverslip onto the silicone gasket and press gently to seal.  
Place gasketed coverslips individually into glass petri dishes (silicone side up), wrap with 
aluminum foil and sterilize by autoclaving.  Allow coverslips to cool and dry completely before 
use. 
 

CultureWell™ MultiWell chambered coverslips are for laboratory use only. 

 

 

Summary of Contents for CultureWell

Page 1: ...as shown in Figure 1 Note Package contents are sterile handle carefully to prevent contamination Figure 1 2 Aseptically peel back the sterile pouch and top plastic liner to expose coverslips as shown in Figures 1 and 2 Figure 2 3 Using a sterile forceps lift the coverslip by grasping the silicone as shown in Figure 3 Figure 3 ...

Page 2: ...he silicone gasketed wells facing up Unused coverslips from opened pouches may be stored in sterile culture dishes to prevent contamination Figure 4 5 Pipette cells into individual wells as shown in Figure 5 Allow the cells to attach for 1 24 hours in a humidified CO2 incubator Wells are spaced 9mm for use with multi channel or automated pipettors Number of Well Chambers Volume μl 8 30 40 4 90 100...

Page 3: ... flooded with a suspension of cells in medium 20ml Plates and incubated to allow cells to attach to coverslips Cells do not attach to the silicone gaskets Figure 6 7 After cultivation cells may be washed fixed and stained in the plate using standard protocols 8 Figure 7 depicts how to remove a MultiWell Coverslip from a plate Grasp the silicone with a forceps and pull slowly upward Figure 7 ...

Page 4: ...ttached to provide stability and protection from breakage Figure 8 shows how individual gasketed coverslips may be placed in coplin jars or any other holder designed for standard microscope slides Figure 8 10 MultiWell coverslips may be placed in culture dishes for antibody hybridization and washing steps Figure 9 Figure 9 ...

Page 5: ...th a mild detergent that is suitable for cell culture Do not soak gaskets in detergent Rinse thoroughly with distilled water and allow gaskets to dry When re using cleaned gaskets just prior to applying them to a new coverslip use a piece of adhesive tape to clean lint and debris from the gasket surface Place a clean dry 24mm x 50mm glass coverslip onto the silicone gasket and press gently to seal...

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