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Grace Bio-Labs CultureWell, Instructions For Use

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Grace Bio-Labs CultureWell Instructions For Use Download Page 4

 

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Instructions for Use 

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CultureWell™ MultiWell Chambered Coverslips

 

 

9.

 

Coverslips are removed with the silicone gasket still attached to provide stability and protection 
from breakage.  Figure 8 shows how individual gasketed coverslips may be placed in coplin jars 
or any other holder designed for standard microscope slides. 

                               

 

 

Figure

 

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MultiWell™ coverslips may be placed in culture dishes for antibody/hybridization and washing 
steps (Figure 9). 

 

 

Figure 9 

Summary of Contents for CultureWell

Page 1: ...as shown in Figure 1 Note Package contents are sterile handle carefully to prevent contamination Figure 1 2 Aseptically peel back the sterile pouch and top plastic liner to expose coverslips as shown in Figures 1 and 2 Figure 2 3 Using a sterile forceps lift the coverslip by grasping the silicone as shown in Figure 3 Figure 3 ...

Page 2: ...he silicone gasketed wells facing up Unused coverslips from opened pouches may be stored in sterile culture dishes to prevent contamination Figure 4 5 Pipette cells into individual wells as shown in Figure 5 Allow the cells to attach for 1 24 hours in a humidified CO2 incubator Wells are spaced 9mm for use with multi channel or automated pipettors Number of Well Chambers Volume μl 8 30 40 4 90 100...

Page 3: ... flooded with a suspension of cells in medium 20ml Plates and incubated to allow cells to attach to coverslips Cells do not attach to the silicone gaskets Figure 6 7 After cultivation cells may be washed fixed and stained in the plate using standard protocols 8 Figure 7 depicts how to remove a MultiWell Coverslip from a plate Grasp the silicone with a forceps and pull slowly upward Figure 7 ...

Page 4: ...ttached to provide stability and protection from breakage Figure 8 shows how individual gasketed coverslips may be placed in coplin jars or any other holder designed for standard microscope slides Figure 8 10 MultiWell coverslips may be placed in culture dishes for antibody hybridization and washing steps Figure 9 Figure 9 ...

Page 5: ...th a mild detergent that is suitable for cell culture Do not soak gaskets in detergent Rinse thoroughly with distilled water and allow gaskets to dry When re using cleaned gaskets just prior to applying them to a new coverslip use a piece of adhesive tape to clean lint and debris from the gasket surface Place a clean dry 24mm x 50mm glass coverslip onto the silicone gasket and press gently to seal...

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