5 Calibration of the OSMOMAT auto
You will now be prompted to insert the sample holder in the unit. The Osmomat auto automatically
detects the inserted sample holder and begins the calibration.
The calibration values are now consecutively shown on the display. If the printer is switched on, these
values are also printed. After successful calibration, the message >
accept calibrationmeas.?
appears.
Confirm the calibration values with
OK
. The unit then automatically prepares for sample measurement.
Otherwise select
repeat
to discard the calibration values and recalibrate the unit.
)
Note: A 2P or 3P is displayed on the right side of the initial display and the displays of the
submenu. These signalize the calibration status of the unit. A 2P indicates two-point
calibration and a 3P indicates three-point calibration. The status changes according to
the calibration performed. This status is retained even after the unit is switched off and
restarted but lost after new initialization.
)
Note: Prior to the calibration procedure, switch the printer on to document the calibration
results. Subsequent display or printing is not possible!
5.2
Calibration at Three Points
Two clean, dry measuring vessels are first filled via a pipette with 50 µl distilled water, 50 µl Gonotec
calibration standard 300 mOsmol/kg, and 50 µl 850 mOsmol/kg.
There must be no visible air bubbles in the liquid!
The sample holder is numbered 0 through 20 from left to right. The sample holder is to be filled as
follows:
Positions
0
and
1
with
distilled water
Positions
2
and
3
with
300 mOsmol/kg
Positions
4
and
5
with
850 mOsmol/kg
Use the proper sequence when filling the sample holder. Incorrect placement results in
incorrect calibration!
The sample cover is then placed on the sample holder to prevent premature evaporation of the
samples.
The sample cover reduces the risk of premature evaporation of the samples. However,
it does not protect the samples from leakage.
Calibration of the OSMOMAT auto
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